2026-05-03T18:08:04Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32149612 2026-05-03T00:00:00Z category_1 category_12 category_16 category_69 category_272 category_734 category_873 category_915 portal_63 item_type_6 month_year_05_2026

Interfacial Energetics of C2–C18 Aliphatic Moieties on Hydrogenated Si(111) and Si(110) Surfaces: A DFT Study Sara Marchio (21015907) Francesco Buonocore (124769) Simone Giusepponi (21015910) Massimo Celino (1512820) Biophysics Cell Biology Physiology Inorganic Chemistry Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified tilted geometry adopted density functional theory charge transfer mechanisms atomic packing density molecular orbital hybridization interfacial electronic structure computational study based alkynyl chains containing engineering interface energetics 18 </ sub 110 ), due exhibits surface dipoles h – si surface dipoles molecular termination longer chains interfacial energetics dft study surface states tunneling transport tunneling barriers terminated si systematically investigate substrate orientation rational choice length dependence hydrogenated si dipole formation crystallographic orientations aliphatic moieties We present a computational study based on density functional theory to systematically investigate how aliphatic moiety functionalization affects the interfacial electronic structure of H-terminated Si(111) and Si(110) surfaces. We explore the energetics, dipole formation, and charge transfer mechanisms for alkyl, alkenyl, and 1-alkynyl chains containing from 2 to 18 carbon atoms chemisorbed on both crystallographic orientations. Our analysis reveals that alkenyl moieties exhibit pronounced chain-length dependence of surface dipoles and tunneling barriers, whereas alkyl and 1-alkynyl chains show saturation effects for longer chains. We found that H–Si(111) exhibits surface dipoles up to 33% larger than H–Si(110), due to differences in atomic packing density and Si–H bond orientation. The resulting charge injection barriers for both thermionic and tunneling transport are quantified and discussed. The tilted geometry adopted by alkenyl moieties on Si(110) is rationalized through analysis of molecular orbital hybridization with surface states. These results provide quantitative guidelines for engineering interface energetics in silicon-based molecular electronic devices through rational choice of molecular termination and substrate orientation. 2026-05-03T00:00:00Z Text Journal contribution 10.1021/acs.langmuir.6c00727.s001 https://figshare.com/articles/journal_contribution/Interfacial_Energetics_of_C_sub_2_sub_C_sub_18_sub_Aliphatic_Moieties_on_Hydrogenated_Si_111_and_Si_110_Surfaces_A_DFT_Study/32149612 CC BY-NC 4.0

oai:figshare.com:article/32149438 2026-05-03T00:00:00Z category_1 category_4 category_12 category_13 category_14 category_16 category_21 category_61 category_128 category_146 category_873 portal_63 item_type_6 month_year_05_2026

Enhanced Expansion of Bone Marrow-Derived Mesenchymal Stem Cells on Collagen-Coated Microcarriers Functionalized with Chimeric IGF-1–bFGF Proteins Shiina Pasanen (23828470) Fumika Abe (23828473) Karina Erda Saninggar (23828476) Isao Hirata (2467459) Kotaro Tanimoto (287296) Koichi Kato (132460) Biophysics Biochemistry Cell Biology Genetics Molecular Biology Physiology Biotechnology Developmental Biology Hematology Space Science Chemical Sciences not elsewhere classified stoichiometric uncertainty inherent molecule design provides functional assays demonstrated fixed molar ratio efficient functionalization strategies defined stoichiometry offer based culture offers chimeric growth factors like growth factor cell transplantation therapies scalable hmsc expansion binding chimeric proteins coated microcarriers functionalized separate proteins scalable platform factor controls enhanced expansion coated poly cell therapy binding peptide chimeric igf vinyl alcohol unique advantage significant implications robust strategy regenerative medicine highest effect fusing insulin findings demonstrate bone marrow Human mesenchymal stem cells (hMSCs) are promising for cell transplantation therapies. Nevertheless, their limited yield necessitates large-scale ex vivo expansion. Microcarrier-based culture offers a scalable platform, but efficient functionalization strategies are not available. Here, we engineered collagen-binding chimeric proteins by fusing insulin-like growth factor-1 (IGF-1) and basic fibroblast growth factor (bFGF) with an osteopontin-derived collagen-binding peptide and immobilized them on type I collagen-coated poly(vinyl alcohol) microcarriers. This single-molecule design provides the unique advantage where the surface is functionalized with a fixed molar ratio of IGF-1 to bFGF, avoiding the stoichiometric uncertainty inherent to the coimmobilization of separate proteins. Structural characterization confirmed proper folding of the chimeras, and functional assays demonstrated that dual-domain constructs markedly enhanced hMSC proliferation compared with single-factor controls, with the IGF-1–bFGF chimera yielding the highest effect. Expanded cells retained osteogenic differentiation potential. These findings demonstrate that chimeric growth factors with defined stoichiometry offer a practical and robust strategy for scalable hMSC expansion, with significant implications for regenerative medicine and cell therapy. 2026-05-03T00:00:00Z Text Journal contribution 10.1021/acsomega.6c01367.s001 https://figshare.com/articles/journal_contribution/Enhanced_Expansion_of_Bone_Marrow-Derived_Mesenchymal_Stem_Cells_on_Collagen-Coated_Microcarriers_Functionalized_with_Chimeric_IGF-1_bFGF_Proteins/32149438 CC BY-NC 4.0

oai:figshare.com:article/32149435 2026-05-03T00:00:00Z category_4 category_734 category_873 portal_63 item_type_6 month_year_05_2026

P450 Cyptide Synthase AscB Catalyzes Fused Cyclophane at the YxWxH Motif on the Precursor Peptides Jabal Rahmat Haedar (22915225) Abujunaid Habib Khan (23828464) Jemma Gullick (18072672) Mathias H. Hansen (12371683) Laura J. Coe (23828467) James J. De Voss (7490135) Viktors Romanuks (20654004) Gints Smits (1916074) Stefano Donadio (774816) Max J. Cryle (1814797) Chin-Soon Phan (3223515) Biochemistry Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified translationally modified peptides precursor peptides cyptides p450 cyptide synthases escherichia coli </ amycolatopsis sacchari </ c – n c – micromonospora </ yxwxh motif unique feature second cyclophane ribosomally synthesized p450s ). new class links formed fused cyclophane functional studies first report dsm 44468 Cyptides are a new class of ribosomally synthesized and post-translationally modified peptides (RiPPs) with the unique feature of biaryl C–C, C–N, or C–O cross-links formed by P450 cyptide synthases (P450s). Through functional studies in Escherichia coli, we discovered two newly identified P450 enzymes, AscB from Amycolatopsis sacchari DSM 44468 and MmsB from Micromonospora sp. HM5-17, that can catalyze the cross-linking between Tyr-C3 and Trp-N1, as well as the hydroxylation at Trp-C5 in the YxWxH motif. However, only AscB was able to catalyze the formation of a second cyclophane between Trp-C4 and His-Nτ, generating a fused cyclophane in the YxWxH motif. This is the first report of an enzyme that can catalyze such cross-linking in RiPPs. 2026-05-03T00:00:00Z Text Journal contribution 10.1021/acs.orglett.6c01044.s001 https://figshare.com/articles/journal_contribution/P450_Cyptide_Synthase_AscB_Catalyzes_Fused_Cyclophane_at_the_YxWxH_Motif_on_the_Precursor_Peptides/32149435 CC BY-NC 4.0

oai:figshare.com:article/32149375 2026-05-03T00:00:00Z category_1 category_4 category_19 category_21 category_272 category_734 category_931 portal_63 item_type_6 month_year_05_2026

Carrier-Regulation in Organic Cathodes for Photochargeable Batteries Boosting High Photoresponse and Long Durability Chang Tong (369266) Huicong Li (5718995) Zhuocheng Tian (18406101) Zhicheng Zhang (490080) Lianmeng Cui (13850074) Dongxue Wang (621725) Liang Zhou (85586) Jianlong Xia (1563811) Biophysics Biochemistry Pharmacology Biotechnology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Information Systems not elsewhere classified yet face challenges sustainable energy conversion smooth electron pathway mismatched charge kinetics enhance charge utilization enables high photostability efficient charge separation cs ), along chemical conversion process 510 mv cm 4 μa cm study introduces carrier acceptor ” structure transformative demonstration sustaining cyclability show promise primarily caused photocathode co organic cathodes multistep solar multiregulation strategy limited cyclicity inefficient photocharging electrochemical processes efficiently shuttled coregulation strategy ca </ 800 times 8 %. Photochargeable batteries (PCBs) show promise for sustainable energy conversion and storage, yet face challenges in inefficient photocharging and limited cyclicity, primarily caused by mismatched charge kinetics in the multistep solar-to-electric-to-chemical conversion process. To address this, we introduce a coregulation strategy that integrates a photocathode featuring electron “Acceptor-Acceptor-Acceptor” structure for efficient charge separation (CS), along with ammonium-based carriers exhibiting rapid transfer dynamics to enhance charge utilization in PCBs. When illuminated, the smooth electron pathway in photocathode yields persistent photogenerated charges that are efficiently shuttled by NH4+ carriers to engage in electrochemical processes. Consequently, this multiregulation strategy with orchestrating carriers and photocathodes produces high photocurrent of 31.4 μA cm–2 and photovoltage of 510 mV cm–2, thereby enhancing reversible capacity of ca. 29.8%. Moreover, low-corrosive NH4+ enables high photostability of PCB, sustaining cyclability over 800 times. This study introduces carrier/photocathode co-regulation as a transformative demonstration for advancing PCBs, complementing existing photoelectrode optimization frameworks. 2026-05-03T00:00:00Z Text Journal contribution 10.1021/acs.nanolett.6c00492.s001 https://figshare.com/articles/journal_contribution/Carrier-Regulation_in_Organic_Cathodes_for_Photochargeable_Batteries_Boosting_High_Photoresponse_and_Long_Durability/32149375 CC BY-NC 4.0

oai:figshare.com:article/32149372 2026-05-03T00:00:00Z category_4 category_21 category_146 category_272 category_734 category_873 category_931 portal_63 item_type_3 month_year_05_2026

Enabling Direct Engineering of Porous Biochar with Machine Learning Using Only Simple Feedstock Information for Efficient Adsorption of CO2 Chengkai Cao (23828401) Zejian Ai (23828404) Weijin Zhang (5431907) Jianan Li (290570) Hao Zhan (6853280) Lijian Leng (3684337) Hailong Li (216327) Biochemistry Biotechnology Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Information Systems not elsewhere classified unified predictive framework mainly focused either machine learning using gradient boosting regression efficiently screen feedstocks coupled machine learning enabling direct engineering simple feedstock information 2 < sup biomass pyrolysis porous biochar performance biochar engineer biochar work provides wide applications validations based sustainable co related areas pyrolysis parameters input variables high co existing studies efficient strategy designing high chemical descriptors capture technologies bean straw 62 mmol Biochar, derived from biomass pyrolysis and activation, is a versatile porous carbon material with wide applications in CO2 adsorption. However, existing studies have mainly focused either on engineering biochar porosity or on correlating biochar properties with CO2 adsorption capacity (CO2Ad-qe), without integrating both physical and chemical descriptors into a unified predictive framework. To address this gap, a coupled machine learning (ML) approach was developed to predict and engineer biochar for CO2 adsorption. A gradient boosting regression (GBR) model was constructed by using biomass composition, pyrolysis parameters, activation conditions, and adsorption conditions as input variables, which achieved excellent predictive performance with an R2 of 0.99 and an RMSE of 0.15. Moreover, the model requires only biomass composition, without any requirements to produce and characterize the biochar, to efficiently screen feedstocks and identify optimal production conditions for engineering biochar with high CO2Ad-qe. Validations based on an extra unseen data set further confirmed the robustness of the model. After screening several hundred biomass samples reported in the literature, bean straw was identified as the most promising feedstock, with a predicted maximum CO2Ad-qe of 6.62 mmol/g. This work provides a data-driven and efficient strategy for designing high-performance biochar, contributing to the advancement of sustainable CO2 capture technologies and related areas. 2026-05-03T00:00:00Z Dataset Dataset 10.1021/acssuschemeng.5c12782.s002 https://figshare.com/articles/dataset/Enabling_Direct_Engineering_of_Porous_Biochar_with_Machine_Learning_Using_Only_Simple_Feedstock_Information_for_Efficient_Adsorption_of_CO_sub_2_sub_/32149372 CC BY-NC 4.0

oai:figshare.com:article/32149369 2026-05-03T00:00:00Z category_4 category_21 category_146 category_272 category_734 category_873 category_931 portal_63 item_type_6 month_year_05_2026

Enabling Direct Engineering of Porous Biochar with Machine Learning Using Only Simple Feedstock Information for Efficient Adsorption of CO2 Chengkai Cao (23828401) Zejian Ai (23828404) Weijin Zhang (5431907) Jianan Li (290570) Hao Zhan (6853280) Lijian Leng (3684337) Hailong Li (216327) Biochemistry Biotechnology Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Information Systems not elsewhere classified unified predictive framework mainly focused either machine learning using gradient boosting regression efficiently screen feedstocks coupled machine learning enabling direct engineering simple feedstock information 2 < sup biomass pyrolysis porous biochar performance biochar engineer biochar work provides wide applications validations based sustainable co related areas pyrolysis parameters input variables high co existing studies efficient strategy designing high chemical descriptors capture technologies bean straw 62 mmol Biochar, derived from biomass pyrolysis and activation, is a versatile porous carbon material with wide applications in CO2 adsorption. However, existing studies have mainly focused either on engineering biochar porosity or on correlating biochar properties with CO2 adsorption capacity (CO2Ad-qe), without integrating both physical and chemical descriptors into a unified predictive framework. To address this gap, a coupled machine learning (ML) approach was developed to predict and engineer biochar for CO2 adsorption. A gradient boosting regression (GBR) model was constructed by using biomass composition, pyrolysis parameters, activation conditions, and adsorption conditions as input variables, which achieved excellent predictive performance with an R2 of 0.99 and an RMSE of 0.15. Moreover, the model requires only biomass composition, without any requirements to produce and characterize the biochar, to efficiently screen feedstocks and identify optimal production conditions for engineering biochar with high CO2Ad-qe. Validations based on an extra unseen data set further confirmed the robustness of the model. After screening several hundred biomass samples reported in the literature, bean straw was identified as the most promising feedstock, with a predicted maximum CO2Ad-qe of 6.62 mmol/g. This work provides a data-driven and efficient strategy for designing high-performance biochar, contributing to the advancement of sustainable CO2 capture technologies and related areas. 2026-05-03T00:00:00Z Text Journal contribution 10.1021/acssuschemeng.5c12782.s001 https://figshare.com/articles/journal_contribution/Enabling_Direct_Engineering_of_Porous_Biochar_with_Machine_Learning_Using_Only_Simple_Feedstock_Information_for_Efficient_Adsorption_of_CO_sub_2_sub_/32149369 CC BY-NC 4.0

oai:figshare.com:article/32149363 2026-05-03T00:00:00Z category_4 category_16 category_21 category_39 category_146 category_734 category_873 category_915 portal_63 item_type_6 month_year_05_2026

3,5-Dinitrobenzoic Acid Enables Stable and Efficient HTL-Free MAPbI3 Perovskite Solar Cells Saravanan Subramani (20539965) Govindaraj Rajamanickam (12440627) Chauhan Anil Kumar (23828398) Biochemistry Physiology Biotechnology Ecology Space Science Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified uncoordinated lead ions present work provides minimizing nonradiative recombination enhancing charge transportation 30 ° c severe surface defects carboxyl (− cooh perovskite solar cells power conversion efficiency benzene ring limit highly efficient cpscs dnbz additive containing 3 </ sub benzene ring reduce defects nitro (− efficient htl perovskite ’ perovskite solution perovskite films term durability straightforward method significant improvement retain 87 investigations reveal hydrophobic properties gained interest free mapbi fabricate inexpensive dinitrobenzoic acid device efficiency cpscs treated based htl 22 days 11 %. 1 mg Hybrid perovskite solar cells have gained interest for their high solar energy efficiency and low-temperature solution processing properties. However, severe surface defects at perovskite grain boundaries still hinder improvements in device efficiency and performance. Here, we effectively introduced organic compound 3,5-dinitrobenzoic acid (DNBZ) as an additive to the perovskite solution. The DNBZ additive containing a benzene ring and carboxyl (−COOH) and nitro (−NO2) functional groups effectively reacts with uncoordinated lead ions (Pb2+) to reduce defects/traps in perovskite films, improving their film quality, minimizing nonradiative recombination, and enhancing charge transportation and the efficiency of CPSCs. Furthermore, the hydrophobic properties of the benzene ring limit the perovskite’s interaction with water, improving the moisture stability of CPSCs. Interestingly, 1 mg-DNBZ added carbon counter electrode-based HTL-free MAPbI3 perovskite solar cells (CPSCs) have a power conversion efficiency (PCE) enhanced from 8.36 to 10.11%. Furthermore, after being kept for 22 days, the PCE of CPSCs treated with 1 mg-DNBZ can retain 87% of its initial PCE (under the condition of 30 °C and RH = 50 ± 5%), indicating a significant improvement in long-term durability. Our investigations reveal that the DNBZ additive strategy improves the stability, efficiency, performance, and quality of perovskite films. The present work provides a straightforward method to fabricate inexpensive and highly efficient CPSCs. 2026-05-03T00:00:00Z Text Journal contribution 10.1021/acs.langmuir.6c00500.s001 https://figshare.com/articles/journal_contribution/3_5-Dinitrobenzoic_Acid_Enables_Stable_and_Efficient_HTL-Free_MAPbI_sub_3_sub_Perovskite_Solar_Cells/32149363 CC BY-NC 4.0

oai:figshare.com:article/32149360 2026-05-03T00:00:00Z category_1 category_4 category_8 category_12 category_16 category_61 category_132 category_134 category_135 category_873 category_915 portal_63 item_type_6 month_year_05_2026

Synthesis of Polymers Consisting of Urea and 3,5-Hydantoin Moieties in an Alternating Manner Based on a Phosgene-Free Method and Their Thermal Properties Tomoya Hayashi (5353106) Haruto Minami (23828395) Yuji Kosugi (2426227) Yasuyuki Mori (3728023) Takehiro Kawauchi (1476997) Yoshio Furusho (2050327) Biophysics Biochemistry Microbiology Cell Biology Physiology Developmental Biology Infectious Diseases Virology Computational Biology Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified unit model compound e ., polycondensation dsc analyses showed alternating manner based hydantoin moiety proceeded alternating manner ureidoacetate moiety utilizing dbu thermomechanical properties thermal properties synthetic method results obtained reaction using n </ main chain ir spectroscopies investigated briefly hydantoin moieties free method first time catalyzed cyclization >′- bis We developed a synthetic method for polymers consisting of urea and 3,5-hydantoin moieties in an alternating manner by utilizing DBU-catalyzed cyclization of the α-ureidoacetate moiety of polyureas, which were prepared using Endo’s phosgene-free method, i.e., polycondensation of N,N′-bis(phenoxycarbonyl) derivatives of ornithine or lysine with diamines. Transformation of the α-ureidoacetate moiety of the polyureas into the 3,5-hydantoin moiety proceeded in DMSO to give the poly(urea-hydantoin)s quantitatively without side reactions. Their structures were confirmed by NMR and FT-IR spectroscopies in combination with results obtained from the reaction using a unit model compound having α-ureidoacetate moiety. Thus, we have achieved the synthesis of polymers with 3,5-hydantoin in the main chain for the first time, whereas polymers bearing 1,3-hydantoin are well-known. Furthermore, TGA and DSC analyses showed that the poly(urea-hydantoin)s had much higher thermal stability than the corresponding polyureas because of the rigidity of the 3,5-hydantoin moieties. Their mechanical and thermomechanical properties were investigated briefly. 2026-05-03T00:00:00Z Text Journal contribution 10.1021/acs.macromol.5c03500.s001 https://figshare.com/articles/journal_contribution/Synthesis_of_Polymers_Consisting_of_Urea_and_3_5-Hydantoin_Moieties_in_an_Alternating_Manner_Based_on_a_Phosgene-Free_Method_and_Their_Thermal_Properties/32149360 CC BY-NC 4.0

oai:figshare.com:article/32149357 2026-05-03T00:00:00Z category_8 category_24 category_39 category_132 category_133 category_134 portal_63 item_type_6 month_year_05_2026

Herbivore-Induced Volatiles Orchestrate Oviposition Plasticity in Geometrid Moths to Counteract Direct Resistance in Tea Plants Yu-Yu Huang (7328012) Zi-Jun Luo (21838127) Ying-Jie Zhao (12469257) Xiao-Ming Cai (2549698) Zong-Xiu Luo (2549701) Lei Bian (1894600) Chun-Li Xiu (17091697) Nan-Xia Fu (23828392) Man-Qun Wang (482845) Zhao-Qun Li (427978) Zong-Mao Chen (2549704) Microbiology Evolutionary Biology Ecology Infectious Diseases Plant Biology Virology specific defensive metabolites mediated metabolomic changes counteract direct resistance coevolutionary arms race avoid defended hosts integrated defense cascade ectropis grisescens ). insect behavioral responses insect behavioral ecology coordinated signaling leads 2 +</ sup synchronized defense grisescens females volatiles serve vitexin ), transgenerational plasticity selective pressure reciprocal adaptations reciprocal adaptation novel paradigm mapk activation jasmonate biosynthesis involving ca intergenerational coevolution gravid e geometrid moths geometrid moth enabling females e </ dynamic equilibrium counterdefense dynamic camellia sinensis >)- β The coevolutionary arms race between plants and herbivores drives the diversification of reciprocal adaptations. However, the transgenerational plasticity of insect behavioral responses to induced plant defenses remains poorly understood. Here, we elucidate a synchronized defense-counterdefense dynamic between tea plants (Camellia sinensis) and the geometrid moth (Ectropis grisescens). We found that larval herbivory rapidly triggers an integrated defense cascade in tea plants, involving Ca2+ signaling, MAPK activation, and jasmonate biosynthesis. This coordinated signaling leads to the accumulation of specific defensive metabolites (luteoloside, galangin, rhofolin, and vitexin), which collectively impair larval growth. Crucially, this herbivore-induced chemical defense creates a selective pressure that drives a reciprocal adaptation in the insect. Gravid E. grisescens females have evolved the ability to detect two characteristic volatiles, (E)-β-ocimene and linalool, emitted by attacked plants. These volatiles serve as reliable oviposition deterrent signals, enabling females to avoid defended hosts and thereby enhance offspring fitness. Our study integrates plant physiological signaling, phytohormone-mediated metabolomic changes, and insect behavioral ecology to reveal a novel paradigm of intergenerational coevolution. It demonstrates how plant induced defenses directly shape insect oviposition strategies, sustaining a dynamic equilibrium in agroecosystems. 2026-05-03T00:00:00Z Text Journal contribution 10.1021/acs.jafc.5c13887.s004 https://figshare.com/articles/journal_contribution/Herbivore-Induced_Volatiles_Orchestrate_Oviposition_Plasticity_in_Geometrid_Moths_to_Counteract_Direct_Resistance_in_Tea_Plants/32149357 CC BY-NC 4.0

oai:figshare.com:article/32149354 2026-05-03T00:00:00Z category_1 category_4 category_14 category_21 category_45 category_132 category_135 category_146 category_502 category_873 portal_63 item_type_6 month_year_05_2026

Freeze-Induced Structural Stabilization of Hydrogel Scaffolds for Dense 3D Printed Ceramics Ge Wang (56838) Xiaohang Wang (4862665) Haoqin Ma (10114816) Shuai Yang (645019) Xinghua Sheng (21585988) Sheng Bi (320166) Nan Sun (788187) Dongchen Tan (10467371) Chengming Jiang (1658248) Biophysics Biochemistry Molecular Biology Biotechnology Sociology Infectious Diseases Computational Biology Space Science Astronomical and Space Sciences not elsewhere classified Chemical Sciences not elsewhere classified preventing structural shrinkage induced structural stabilization conventional vat photopolymerization advanced engineering applications complex ceramic structures advanced ceramic materials specific photocurable resins metal precursors within manufacturing complex architectures high structural fidelity ceramic precursor incorporation decoupling structural fabrication ceramic densification precursor infiltration high viscosity filled resins adsorption within additive manufacturing target geometry scalable route repeated optimization progressively increased powerful platform phase transition overall volume ordered microstructures microstructural compactness light scattering hydrogel scaffolds hydrogel network hydrogel framework frozen tofu ceramics provides assisted hydrogel Three dimensional (3D) printing of ceramics provides a powerful platform for manufacturing complex architectures for advanced engineering applications. However, conventional vat photopolymerization-based ceramic printing relies on particle-filled resins, where light scattering, high viscosity, and material-specific formulation constraints significantly limit printing precision and material versatility. Here we report a freeze-assisted hydrogel-derived ceramic manufacturing method that enables the fabrication of dense 3D ceramic architectures while decoupling structural fabrication from ceramic precursor incorporation. In this approach, a photocured hydrogel scaffold first defines the target geometry, followed by precursor infiltration and a repeated soak–freeze–dry cycle inspired by the formation mechanism of frozen tofu. During freezing, the phase transition of water generates ice crystals that stabilize the hydrogel network and preserve its overall volume, preventing structural shrinkage and collapse. Simultaneously, ice-crystal growth produces interconnected porous channels that significantly enhance precursor transport and adsorption within the scaffold. Through repeated infiltration–freezing cycles, the concentration of metal precursors within the hydrogel framework is progressively increased, enabling the formation of dense and structurally stable ceramic architectures after calcination. This method effectively improves precursor distribution and ceramic densification while eliminating the need for repeated optimization of material-specific photocurable resins. As a result, a variety of complex ceramic structures can be fabricated with high structural fidelity and ordered microstructures. The resulting ceramics exhibit improved densification and microstructural compactness, demonstrating a versatile and scalable route for the additive manufacturing of advanced ceramic materials. 2026-05-03T00:00:00Z Text Journal contribution 10.1021/acsaenm.6c00311.s001 https://figshare.com/articles/journal_contribution/Freeze-Induced_Structural_Stabilization_of_Hydrogel_Scaffolds_for_Dense_3D_Printed_Ceramics/32149354 CC BY-NC 4.0 eyJtZXRhZGF0YVByZWZpeCI6ICJvYWlfZGMiLCAidmVyYiI6ICJMaXN0UmVjb3JkcyIsICJvYWlfaGFuZGxlciI6IHRydWUsICJpbnN0aXR1dGlvbl9pZCI6IDYzLCAicGFnZSI6IDIsICJ4Q3Vyc29yIjogImV5SmhiR2NpT2lKSVV6VXhNaUo5Lklsc3hOemMzT0RFek5EazVNREF3TENBek1qRTBPVE0xTkYwaS5sdEwzYkNFV0E4NE1XYkFEVElscDdlZW9sS3k1bU55NGVfRDJiTnVFUFgyUW1kOWhkZ1VsczJiVEh0TG0wN0hadDhfdWVyNmM5WnpRaXpDYlpFdjdwdyIsICJleHBpcmF0aW9uIjogIjIwMjYtMDUtMDMgMTk6MDg6MDQifQ==