2026-08-07T04:57:59Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33181128 2026-08-07T04:03:39Z category_1 category_4 category_7 category_13 category_15 category_19 category_21 category_46 category_64 category_734 category_873 portal_63 item_type_6 month_year_08_2026

Discovery of High-Affinity Peptide Ligands Targeting the SPIN1 Tudor 3 Domain Via a Minimalist Pruning Strategy Alessandra Feoli (1629745) Giulio Esposito (735927) Manuela Grimaldi (680669) Monica Viviano (1828375) Ida Pacilio (24530676) Josef Krátký (24530679) Anna Maria D’Ursi (654019) Ciro Milite (1629742) Haitao Li (305080) Mark T. Bedford (182601) Sabrina Castellano (560784) Gianluca Sbardella (1339251) Biophysics Biochemistry Medicine Genetics Neuroscience Pharmacology Biotechnology Immunology Cancer Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified orthogonal biophysical validation minimalist pruning strategy epigenetic reader involved affinity chemical probes mer peptide docpep3 parent peptide significantly outperforming g ., core pharmacophore Spindlin1 (SPIN1) is an epigenetic reader involved in oncology, whose third Tudor domain remains largely underexplored. Here, we applied a minimalist pruning strategy to the 26-mer peptide DOCpep3 to identify the core pharmacophore for SPIN1 Tudor 3 domain binding. This yielded truncated linear peptide analogues (e.g., <b>1</b>, <b>3</b>, and <b>4</b>) displaying low nanomolar affinities, significantly outperforming the parent peptide. Orthogonal biophysical validation (MST and SPR) confirmed competitive target engagement. Furthermore, circular dichroism spectroscopy revealed that these pruned ligands induce distinct structural rearrangements in SPIN1, establishing high-affinity chemical probes for drug discovery. 2026-08-07T00:00:00Z Text Journal contribution 10.1021/acsmedchemlett.6c00384.s001 https://figshare.com/articles/journal_contribution/Discovery_of_High-Affinity_Peptide_Ligands_Targeting_the_SPIN1_Tudor_3_Domain_Via_a_Minimalist_Pruning_Strategy/33181128 CC BY-NC 4.0

oai:figshare.com:article/33180694 2026-08-07T03:13:15Z category_7 category_12 category_21 category_146 category_734 portal_63 item_type_6 month_year_08_2026

Smartphone Imaging-Based Luminol Electrochemiluminescence at the Cobalt Electrode for Sinapic Acid Determination Yu Zheng (2950) Jiyang Liu (1336308) Hongzhan Liu (4182934) Jinwei Xiao (24530038) Vivien Yi Mian Jong (9276308) Yiran Guan (8630520) Guobao Xu (1788382) Medicine Cell Biology Biotechnology Space Science Biological Sciences not elsewhere classified sinapic acid determination related interfacial reactions reactive oxygen intermediates proposed system showed active transition metal portable analytical method based luminol electrochemiluminescence electrode reactions associated portable luminol electrochemiluminescence ecl signal recorded cobalt electrode generated bare cobalt electrode smartphone imaging system cobalt electrode portable strategy smartphone imaging fiber electrode electrode platform luminol ecl imaging parameters cobalt electrodes tested conditions strong promotion site sensing site analysis satisfactory recoveries results demonstrate optimized conditions linear range interference capability first time experimental conditions current two alkaline media 26 μm Developing simple and portable luminol electrochemiluminescence (ECL) systems without externally added co-reactants is important for on-site analysis. Cobalt is a low-cost and redox-active transition metal with favorable electrocatalytic properties toward water-oxidation-related interfacial reactions in alkaline media, which may facilitate luminol oxidation and the generation or activation of reactive oxygen intermediates during ECL. In this work, for the first time, a bare cobalt electrode was introduced into a co-reactant-free luminol ECL system and used to construct a simple direct-current two-electrode platform, with the ECL signal recorded by smartphone imaging. Compared to a carbon-fiber electrode, the cobalt electrode generated a much stronger luminol ECL signal, with an enhancement of more than 80-fold in the smartphone imaging system, indicating its strong promotion of the electrode reactions associated with luminol ECL. After optimization of the imaging parameters and experimental conditions, a portable analytical method for sinapic acid determination was established. Under the optimized conditions, the proposed system showed a linear range of 1–100 μM and a limit of detection of 0.26 μM. The method also showed acceptable anti-interference capability under the tested conditions and satisfactory recoveries in spiked white mustard seed samples. These results demonstrate a simple, low-cost, and portable strategy for co-reactant-free luminol ECL analysis and highlight the potential of cobalt electrodes for proof-of-concept on-site sensing. 2026-08-06T00:00:00Z Text Journal contribution 10.1021/acs.langmuir.6c02784.s001 https://figshare.com/articles/journal_contribution/Smartphone_Imaging-Based_Luminol_Electrochemiluminescence_at_the_Cobalt_Electrode_for_Sinapic_Acid_Determination/33180694 CC BY-NC 4.0

oai:figshare.com:article/33180691 2026-08-07T03:12:09Z category_15 category_21 category_24 category_39 category_132 category_146 category_734 category_873 portal_63 item_type_6 month_year_08_2026

A Novel Reactive Hindered Amine Light Stabilizer for In Situ Grafting in POE Photovoltaic Encapsulation Films during Cross-Linking Zhipeng Liu (380923) Wei Cui (92129) Pinzhe Luo (23053501) Shicheng Zhao (815876) Neuroscience Biotechnology Evolutionary Biology Ecology Infectious Diseases Space Science Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified surface nitrogen content storage modulus induced severe service conditions ray photoelectron spectroscopy prolonged thermal treatment photovoltaic encapsulation films new technical strategy linked network degradation h nmr ). 1 </ sup enhanced antiphotoaging property enhanced antiphotoaging properties indicating enhanced resistance poe encapsulation films significantly reduced migration xps analysis revealed rhals effectively reduced antiphotoaging requirements effectively mitigated photoaging revealed poe backbone photoaging resistance thereby broadening successful synthesis study offers situ grafting similar structures rheological studies results demonstrate potential applications polyolefin elastomer often fail mechanism responsible grafted onto carbonyl species Conventional hindered amine light stabilizers (HALS) often fail to meet the antiphotoaging requirements for Polyolefin Elastomer (POE) photovoltaic encapsulation films under harsh environmental conditions due to migration and leaching. In this research, a novel reactive hindered amine light stabilizer (rHALS) for in situ grafting during cross-linking of POE photovoltaic encapsulation films was synthesized and its performance in enhancing the photoaging resistance of POE encapsulation films was investigated. First, the successful synthesis of rHALS was confirmed by Fourier Transform Infrared Spectroscopy (FTIR) and H Nuclear Magnetic Resonance spectroscopy (¹H NMR). Subsequently, rHALS was employed to enhance the photoaging resistance of POE encapsulation films. FTIR analysis after photoaging revealed that rHALS effectively reduced the formation of carbonyl species (with a 42% reduction in carbonyl peak area), indicating significant inhibition of photoaging. Rheological studies further demonstrated that rHALS more effectively mitigated the decline in the storage modulus induced by photoaging compared to conventional HALS with similar structures, indicating enhanced resistance to cross-linked network degradation and thus enhanced antiphotoaging performance. Finally, the mechanism responsible for the enhanced antiphotoaging property of rHALS was investigated. Both FTIR and X-ray photoelectron spectroscopy (XPS) results confirmed that rHALS was grafted onto the POE backbone during cross-linking. This chemical bonding rendered rHALS more resistant to migration and leaching. XPS analysis revealed that after prolonged thermal treatment, the surface nitrogen content (indicative of HALS leaching) increased by 33% in POE films using conventional HALS, whereas those with rHALS showed only a 4% increase. These results demonstrate that rHALS possesses a stronger antiphotoaging performance due to its significantly reduced migration and leaching. This study offers a new technical strategy for designing POE encapsulation films with enhanced antiphotoaging properties, thereby broadening their potential applications under severe service conditions. 2026-08-06T00:00:00Z Text Journal contribution 10.1021/acs.iecr.6c02733.s001 https://figshare.com/articles/journal_contribution/A_Novel_Reactive_Hindered_Amine_Light_Stabilizer_for_In_Situ_Grafting_in_POE_Photovoltaic_Encapsulation_Films_during_Cross-Linking/33180691 CC BY-NC 4.0

oai:figshare.com:article/33180688 2026-08-07T03:11:38Z category_4 category_7 category_8 category_12 category_14 category_46 category_61 category_132 category_146 portal_63 item_type_6 month_year_08_2026

Cross-Kingdom miR5054 Delivery via Polygonum cuspidatum-Derived Nanoparticles Enhances Burn Wound Healing by Regulating Microvascular Environment Qi Xiu (14845616) Weilun Pan (5391650) Bodeng Wu (16336388) Peiling Chen (1355955) Ningcen Li (11216364) Yingjing Fan (16541624) Chen Li (54018) Junjie Feng (135572) Jiangang Mei (7540703) Xiuhua Wu (5783882) Mingzhen Zhong (16336394) Shan Lin (228596) Wenting Chen (1979884) Bo Li (112195) Lei Zheng (106363) Biochemistry Medicine Microbiology Cell Biology Molecular Biology Immunology Developmental Biology Infectious Diseases Space Science κb signaling axis wound immune microenvironment upon endothelial delivery thermosensitive hydrogel formulated silences keap1 mrna resultant dual amelioration polygonum cuspidatum </ polarizing macrophages toward natural combinatorial therapy enhanced collagen remodeling advanced wound management >- derived nanoparticles severely impedes healing nrf2 antioxidant pathway work unveils pdns mir5054 directly targets healing m2 phenotype nrf2 activation cross healing efficacy sustained activation therapeutic effect simultaneously disrupt sequence inhibitor scavenging metabolites regenerative niche perpetuating cycle pathogenic loop multifaceted nanotherapeutic inflammatory nf inflammatory cascade indispensable role genetic components functional microrna codelivers chemical burn models biomimetic strategy Deep partial-thickness burns are characterized by a self-perpetuating cycle of oxidative stress and inflammation that severely impedes healing. Here, we identified <i>Polygonum cuspidatum</i>-derived nanoparticles (PDNs) as a multifaceted nanotherapeutic that breaks this pathogenic loop. We found that PDNs coencapsulate immediate radical-scavenging metabolites with a functional microRNA, miR5054. Upon endothelial delivery, miR5054 directly targets and silences Keap1 mRNA, leading to sustained activation of the Nrf2 antioxidant pathway. This Nrf2 activation cross-inhibits the pro-inflammatory NF-κB signaling axis in endothelium. The resultant dual amelioration of oxidative and inflammatory stress potently reprograms the wound immune microenvironment, polarizing macrophages toward a pro-healing M2 phenotype. In burn models, PDNs accelerated wound closure, enhanced collagen remodeling, and fostered a Nrf2-high, M2-dominant pro-regenerative niche. The therapeutic effect of PDNs was abolished by a complementary-sequence inhibitor of miR5054, establishing its indispensable role. Furthermore, a synthetic miR5054 mimic recapitulated these benefits, while a thermosensitive hydrogel formulated for PDN delivery enhanced skin penetration and healing efficacy. Our work unveils PDNs as a natural combinatorial therapy that codelivers chemical and genetic components to simultaneously disrupt the oxidative-inflammatory cascade in burns, presenting a translatable, biomimetic strategy for advanced wound management. 2026-08-06T00:00:00Z Text Journal contribution 10.1021/acsnano.6c08834.s001 https://figshare.com/articles/journal_contribution/Cross-Kingdom_miR5054_Delivery_via_Polygonum_cuspidatum-Derived_Nanoparticles_Enhances_Burn_Wound_Healing_by_Regulating_Microvascular_Environment/33180688 CC BY-NC 4.0

oai:figshare.com:article/33180685 2026-08-07T03:11:01Z category_7 category_12 category_15 category_21 portal_63 item_type_6 month_year_08_2026

Flexible Multimodal Neural Probe with Integrated Three-Electrode Aptameric Sensing for In Vivo Monitoring of Dopamine Dynamics and Neural Activity Szu-Ying Li (4019288) Yun-Ting Kuo (10915128) Sheng-Huang Lin (8040437) Shun-An Kan (24530035) Bo-Wei Chen (1837429) Ssu-Ju Li (6475133) Ching-Wen Chang (15278) Han-Lin Wang (8638884) Yu-Chun Lo (1731784) You-Yin Chen (425921) Medicine Cell Biology Neuroscience Biotechnology wave voltammetry frequency specific molecular recognition lack molecular specificity fold ecsa enhancement electrode aptameric sensing controlling gold nanostructure acute rat caudate electrophysiological recording sites electrophysiological electrodes exhibited increased neuronal firing chip sensing system acute multimodal neurochemical 65 v provided 100 hz providing 02 ± 18 specific da recognition labeled da aptamer sensor demonstrated sequence electrochemical impedance analysis auns working electrodes 2 </ sup vivo </ italic vivo </ electrophysiological interrogation r </ neuronal activity neurochemical signaling mean impedance electrochemical interface chip three >< sup 70 v 200 hz 02 %. systematically optimized putamen experiments optimized conditions methylene blue interprobe cv integrated three integrated ag extracellular dopamine experimentally optimized electroactive interferents electrical isolation electrical activity cv %) Neural function emerges from the interplay between electrical activity and neurochemical signaling, yet most implantable neural interfaces primarily record electrophysiological signals and lack molecular specificity for neurotransmitter monitoring. Here, we developed a flexible multimodal neural probe integrating an on-chip three-electrode electrochemical aptamer sensor with electrophysiological recording sites for combined monitoring of extracellular dopamine (DA) dynamics and neuronal activity. Fabrication of the electrochemical interface was systematically optimized by controlling gold nanostructure (AuNS) electrodeposition from 0.50 to 0.70 V. Electrodeposition at 0.65 V provided the best balance between increased electrochemically active surface area (ECSA), surface morphology, fabrication reproducibility, and electrical isolation, yielding an approximately 4.7-fold ECSA enhancement with a probe-to-probe coefficient of variation (CV%) of 2.02%. A methylene blue-labeled DA aptamer was assembled onto the AuNS working electrodes for sequence-specific molecular recognition, while integrated Ag/AgCl reference and nanostructured platinum counter electrodes completed the on-chip sensing system. Electrochemical impedance analysis further demonstrated reproducible interfacial characteristics following stepwise functionalization. The electrophysiological electrodes exhibited a mean impedance of 366.02 ± 18.63 kΩ at 1 kHz with an interprobe CV% of 5.09%. Square-wave voltammetry frequency was experimentally optimized from 10 to 200 Hz, with 100 Hz providing the best balance between analytical response, background current, and reproducibility. Under optimized conditions, the sensor demonstrated sequence-specific DA recognition, selectivity against electroactive interferents, and an experimentally determined detection limit of 10 fM. Two absolute linear response regions were identified at 0.5–10 pM (<i>R</i>² = 0.9945) and 0.5–10 nM (<i>R</i>² = 0.9949), while the broader detectable concentration range extended from 10 fM to 1 μM. Flow-injection and brain-phantom experiments demonstrated reversible DA sensing. In acute rat caudate-putamen experiments, intravenous nomifensine induced DA-associated electrochemical responses accompanied by increased neuronal firing and γ/high-γ local field potential activity, supporting this platform for acute multimodal neurochemical and electrophysiological interrogation <i>in vivo</i>. 2026-08-06T00:00:00Z Text Journal contribution 10.1021/acschemneuro.6c00550.s001 https://figshare.com/articles/journal_contribution/Flexible_Multimodal_Neural_Probe_with_Integrated_Three-Electrode_Aptameric_Sensing_for_In_Vivo_Monitoring_of_Dopamine_Dynamics_and_Neural_Activity/33180685 CC BY-NC 4.0

oai:figshare.com:article/33180680 2026-08-07T03:10:12Z category_1 category_4 category_7 category_15 category_21 category_146 category_873 portal_63 item_type_6 month_year_08_2026

Intramolecular Heterojunctions for Highly Sensitive Room-Temperature NO₂ Sensing Guangling Liang (24530030) Wanqi Dong (14230919) Yuan Lin (79040) Xiaoqing Yu (86297) Gang Xu (219455) Guane Wang (24530033) Biophysics Biochemistry Medicine Neuroscience Biotechnology Space Science Chemical Sciences not elsewhere classified toward 10 ppm thereby substantially enhancing theoretical calculations reveal recovery time toward low detection limit limited contact areas contact heterojunctions suffer construct two intramolecular via cu – strong internal built highly sensitive room higher response value 2 </ sub intramolecular heterojunctions ultimately leading traditional particle term stability study establishes stronger ief sensing performance restricted gas ief ). heterostructured frameworks gas sensors gas sensing faster response excellent long electric fields electric field coordination bonds carrier separation 05 ppb Heterojunction materials achieve efficient charge transfer and carrier separation through built-in- electric fields at the interfaces, thereby substantially enhancing the sensitivity and selectivity of gas sensors. However, traditional particle-contact heterojunctions suffer from discontinuous interfaces and limited contact areas, resulting in low charge transport efficiency and restricted gas-sensing performance. Herein, we construct two intramolecular D–A heterostructured frameworks (CuBr-CuMND and CuI-CuMND) by directionally linking CuX chains (donor) and CuMND (acceptor) via Cu–S coordination bonds. The atomically continuous heterointerface achieves spatial highest occupied molecular orbital/lowest unoccupied molecular orbital separation and a strong internal built-in electric field (IEF). CuI-CuMND exhibits a record-low detection limit of 0.05 ppb and a response of 1315% toward 10 ppm of NO₂, along with excellent long-term stability at room temperature without light assistance. Theoretical calculations reveal that, compared with CuBr-CuMND, CuI-CuMND exhibits a larger Fermi level disparity, which generates a stronger IEF that significantly enhances interfacial charge separation and transport, ultimately leading to a higher response value and faster response/recovery time toward NO₂. This study establishes a molecular-scale intramolecular heterojunction engineering strategy for room-temperature trace-gas sensing. 2026-08-06T00:00:00Z Text Journal contribution 10.1021/acs.inorgchem.6c02751.s001 https://figshare.com/articles/journal_contribution/Intramolecular_Heterojunctions_for_Highly_Sensitive_Room-Temperature_NO_sub_2_sub_Sensing/33180680 CC BY-NC 4.0

oai:figshare.com:article/33180677 2026-08-07T03:09:23Z category_7 category_8 category_39 category_46 category_64 category_132 category_134 category_135 category_915 portal_63 item_type_6 month_year_08_2026

Larval Exposure Rather than Adult Worm Burden Shapes Systemic and Mucosal Th2 Immunity in Ascaris suum Infected Pigs Larissa Oser (21811115) Sara Roose (11485985) Ankur Midha (5600645) Josephine Schlosser-Brandenburg (11278116) Alexandra Laubschat (21811112) Zaneta D. Musimbi (16620264) Arkadi Kundik (14097003) Philipp Höfler (24530024) Christina S. Helm (24530027) Georg von Samson-Himmelstjerna (225285) Peter Geldhof (147755) Susanne Hartmann (233548) Friederike Ebner (5632439) Medicine Microbiology Ecology Immunology Cancer Infectious Diseases Virology Computational Biology Physical Sciences not elsewhere classified worm load independent tfh cell responses suum larval migration suum body migration significant human morbidity mucosal th2 immunity mimicking natural settings local type 2 larval exposure rather high intragroup variability found systemic gata3 differ detectably across adult worm population adult worm burden prevalent parasitic infection suum infected pigs suum antigens showed bolus infected animals applying different doses eosinophils increased dose adult worms trickle infection infection schemes using trickle still unclear specifically recognize small doses porcine industry often studied infective eggs experimental infections economic losses data suggest bolus infections antiparasite response Ascariasis is a prevalent parasitic infection in both humans and pigs, resulting in significant human morbidity and economic losses in the porcine industry. Experimental infections are often studied by mimicking natural settings, exposing the host to frequent, small doses (trickle infection) or by administering a single (bolus) dose of infective eggs. However, the impact of infection schemes and doses on the host immune response inA. suum infected pigs is still unclear. Using trickle and bolus infections and applying different doses, we found systemic GATA3+CD4+ T cells and eosinophils increased dose-dependently during A. suum body migration, while CD4+ T cells that specifically recognize A. suum antigens showed no association with the infection dose. In contrast, all dose-dependent differences were resolved by 8–9 weeks post infection, when adult worms were present. The results further showed that adult worm burden did not differ detectably across all groups, though a high intragroup variability was observed. Local type 2, germinal center B cell, and Tfh cell responses were dose-dependent in bolus infected animals only. Our data suggest that the initial immune response generated during A. suum larval migration is pivotal in determining the fate of the antiparasite response and adult worm population. Moreover, our results indicate that the host can regulate the worm load independent of the infection dose and scheme. 2026-08-06T00:00:00Z Text Journal contribution 10.1021/acsinfecdis.6c00176.s001 https://figshare.com/articles/journal_contribution/Larval_Exposure_Rather_than_Adult_Worm_Burden_Shapes_Systemic_and_Mucosal_Th2_Immunity_in_Ascaris_suum_Infected_Pigs/33180677 CC BY-NC 4.0

oai:figshare.com:article/33180674 2026-08-07T03:08:38Z category_1 category_4 category_8 category_14 category_21 category_133 category_734 category_873 category_915 portal_63 item_type_6 month_year_08_2026

Bioinspired Large-Area Polymeric Cucurbituril Monolayers with Ultrahigh-Density Nanopores Enabling Reversible Charge-Gating of Ion Transport via Host–Guest Recognition Yehui Ding (24530009) Meng He (730322) Jianhui Lan (1269657) Fuzhu Liu (748212) Dario Calvani (18002113) Peng Lin (251745) Yingzhe Du (8443329) Jun Sun (48981) Xiangdong Ding (122577) Weiqun Shi (1517995) Xue Liu (420033) Biophysics Biochemistry Microbiology Molecular Biology Biotechnology Plant Biology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified osmotic power generation introducing dynamic functionality intrinsic cavities act delivers outstanding performance cucurbituril hosts serve covalent organic frameworks advanced energy harvesting dense transport pathways subnanometer channels together 5 ± 0 2 </ sup 13 </ sup subnanometer pores subnanometer confinement × 10 work provides supramolecular assemblies responsive separation remains challenging potential applications nanopores formed molecular transport membrane science level control interfacial cross high density design strategy cofs ). bioinspired strategy area membranes Achieving precise control over ion and molecular transport under subnanometer confinement, especially beyond passive sieving, remains challenging in nanofluidics and membrane science. Here, we present a bioinspired strategy that enables the formation of subnanometer channels together with the integration of tunable molecular recognition within 2D amorphous polymer membranes. Large-area membranes with a size up to 900 cm² were fabricated by interfacial cross-linking of supramolecular assemblies. Molecular-level control over the amphiphilicity of cucurbituril host–guest complexes (HGCs) yields 1.6 nm thick amorphous monolayers with subnanometer pores at a high density of (1.5 ± 0.5) × 10¹³ cm^–2, comparable to that of covalent organic frameworks (COFs). The nanopores formed between cucurbituril hosts serve as dense transport pathways, while the intrinsic cavities act as dynamic host–guest recognition sites. This architecture enables reversible charge-gating of ion transport via host–guest recognition and delivers outstanding performance in osmotic power generation. This work provides a design strategy for constructing adaptive transport systems, introducing dynamic functionality into otherwise static porous architectures, with potential applications for responsive separation and advanced energy harvesting. 2026-08-06T00:00:00Z Text Journal contribution 10.1021/jacs.6c07373.s001 https://figshare.com/articles/journal_contribution/Bioinspired_Large-Area_Polymeric_Cucurbituril_Monolayers_with_Ultrahigh-Density_Nanopores_Enabling_Reversible_Charge-Gating_of_Ion_Transport_via_Host_Guest_Recognition/33180674 CC BY-NC 4.0

oai:figshare.com:article/33180671 2026-08-07T03:08:08Z category_8 category_16 category_39 category_133 category_272 category_734 category_873 portal_63 item_type_6 month_year_08_2026

Freeze–Thaw Cycles Greatly Affect the Conjugative Transfer of Antibiotic-Resistant Genes: Effects of the Secretion of Extracellular Polymeric Substances Zhengmao Li (12274533) Jianmei Qin (19207985) Lei He (143930) Chenyi Nie (12274536) Fuyang Liu (5136770) Yanghui Hou (14025832) Meiping Tong (643650) Microbiology Physiology Ecology Plant Biology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified reduced conjugation frequency predict arg risks ongoing climate change energy deficiency caused despite ionic strengths arg conjugation frequency 5 – 76 study clearly show horizontal gene transfer exhibit profound influence important physical process different natural environments args ), yet 2 – 27 1 ft cycle 3 ft cycles natural environments study investigated may influence ft cycles conjugative transfer ft treatment starvation process species pairs resistant genes primarily promoting mainly attributed inhibition effect increased hydrophobicity helps understand facilitation effect conjugative pilus Freeze–thaw (FT) cycle, a ubiquitous and important physical process in natural environments, may influence the horizontal gene transfer (HGT) process of antibiotic-resistant genes (ARGs), yet its impact and underlying mechanisms remain unclear. This study investigated the impact and mechanisms of FT cycles on ARG conjugative transfer among intra- and interspecies under different solution ionic strength conditions. The results showed that despite ionic strengths and species pairs, different FT treatment cycles induced diverse influences on ARG conjugation frequency with inhibition effect of 1 FT cycle (decreased by 3.5–76-fold) and facilitation effect of 2 and 3 FT cycles (promoted by 2–27-fold). The reduced conjugation frequency after 1 FT treatment cycle is mainly attributed to energy deficiency caused by the starvation process during FT treatment. The enhanced cell–cell adhesion induced by the increased hydrophobicity and secretion of bacterial extracellular polymeric substances (EPS) facilitated the formation of conjugative pilus, primarily promoting the ARG conjugative transfer process after 2 and 3 FT treatment cycles. The results of our study clearly show that FT cycles (intensified by the ongoing climate change) exhibit profound influence on the dissemination of ARGs, which helps understand and predict ARG risks in different natural environments. 2026-08-06T00:00:00Z Text Journal contribution 10.1021/acs.est.6c06057.s001 https://figshare.com/articles/journal_contribution/Freeze_Thaw_Cycles_Greatly_Affect_the_Conjugative_Transfer_of_Antibiotic-Resistant_Genes_Effects_of_the_Secretion_of_Extracellular_Polymeric_Substances/33180671 CC BY-NC 4.0

oai:figshare.com:article/33180668 2026-08-07T03:07:17Z category_4 category_8 category_12 category_19 category_21 category_146 category_272 category_734 category_873 portal_63 item_type_6 month_year_08_2026

A Greener Approach toward Ynones via Visible-Light-Induced Oxidation of Propargyl Alcohols under Aqueous-Aerobic Conditions Biman Bera (24529955) Arabinda Halder (24529958) Pradeep Kumar (310858) Prema G. Vasudev (2378164) Musa A. Said (2939058) Mrinal K. Bera (1427299) Biochemistry Microbiology Cell Biology Pharmacology Biotechnology Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified spatial vector divergence reference pkc inhibitor optimized buried volume method also indicates exit vector analysis dimensional bulk distribution vcam1 analogues successful gram substituent orientation steric mapping scale experiment propargyl alcohols propargyl alcohol photoinduced protocol mediated oxidation industrial applications excellent yield diverse range closely mimics clearer insight A simple and convenient approach involving visible-light-induced, TBHP-mediated oxidation of propargyl alcohols to ynone in aqueous media has been developed. This photoinduced protocol was conducted under an aerobic-aqueous medium using Eosin Y as a photosensitizer. A diverse range of ynone derivatives were prepared from propargyl alcohol in moderate to excellent yield. Furthermore, the successful gram-scale experiment of this method also indicates its flexibility and applicability for industrial applications. Steric mapping of the series <b>2a</b>–<b>2q</b>, exemplified by <b>2j</b>, revealed an optimized buried volume and three-dimensional bulk distribution that closely mimics the reference PKC inhibitor while surpassing the less efficient steric profiles of the MSY and VCAM1 analogues. In addition, an exit vector analysis was carried out to get a clearer insight into the substituent orientation and spatial vector divergence. 2026-08-06T00:00:00Z Text Journal contribution 10.1021/acs.joc.6c00757.s001 https://figshare.com/articles/journal_contribution/A_Greener_Approach_toward_Ynones_via_Visible-Light-Induced_Oxidation_of_Propargyl_Alcohols_under_Aqueous-Aerobic_Conditions/33180668 CC BY-NC 4.0 eyJtZXRhZGF0YVByZWZpeCI6ICJvYWlfZGMiLCAidmVyYiI6ICJMaXN0UmVjb3JkcyIsICJvYWlfaGFuZGxlciI6IHRydWUsICJpbnN0aXR1dGlvbl9pZCI6IDYzLCAicGFnZSI6IDIsICJ4Q3Vyc29yIjogIklsc3hOemcyTURjeU1ETTNNREF3TENBek16RTRNRFkyT0YwaS4yYlNsRHRJckY2d2g1Z2tMWllnSWlNU2ZWdWsiLCAiZXhwaXJhdGlvbiI6ICIyMDI2LTA4LTA3IDA1OjU3OjU5In0=