2026-04-29T12:45:27Z https://api.figshare.com/v2/oai

oai:figshare.com:article/31486840 2026-04-29T12:44:37Z portal_1114 item_type_8 month_year_04_2026

31486840 Differentiation of human and murine CD8+ T cells and their related functional properties <p dir="ltr">CD8+ T cells are very important in clearing pathogen and providing long-term protective immunity. However, T cell responses to antigen are highly diverse, and the differentiation state of the T cells is one major determinant of this functional heterogeneity. Upon antigen encounter, antigen-specific CD8+ T cells clonally expand and contribute to pathogen clearance by eliminating infected cells. Following clearance, most antigen- experienced CD8+ T cells go through cell death while a small population persists as long-lived memory T cells. These cells are very important for a strong recall response upon re-encounter of the same pathogen. Understanding the molecular mechanisms underlying the functional and phenotypic diversity of T cells is critical for the development of effective vaccines and T cell-based therapies, as it enables the identification and selective induction of T cell subsets with optimal effector function, persistence, and protective capacity. This thesis aims to understand T cell differentiation and how T cell differentiation states relate to functional properties.</p><p dir="ltr">By using high-dimensional approaches and studying T cell differentiation as a continuous process rather than discrete subsets, we identified the chemokine receptor CX3CR1 as a robust differentiation marker that captures the continuum of T cell differentiation in both human and mouse. We further demonstrated that CX3CR1 expression correlated with the functional properties of T cells, such as cytotoxic potential, cytokine production, and proliferative capacity, and reflects comparable functional states across species, thereby facilitating translation of findings across species. Moreover, by using CX3CR1 expression, we also unraveled mechanistic linkage between migratory behavior and homeostatic proliferation, which is necessary for the maintenance of memory T cell pool. Finally, in this thesis, we also identified micro-environmental factors that influence the induction and maintenance of CX3CR1 expression in memory CD8+ T cells in vitro, highlighting contexts in which CX3CR1 may be less reliable as a differentiation marker.</p><h3 dir="ltr">List of scientific papers</h3><p dir="ltr">I. Graded expression of the chemokine receptor CX3CR1 marks differentiation states of human and murine T cells and enables cross-species interpretation. Anthonie Johan Zwijnenburg, <b>Jyoti Pokharel</b>, Renata Varnaitė, Wenning Zheng, Elena Hoffer, Iman Shryki, Natalia Ramirez Comet, Marcus Ehrström, Sara Gredmark-Russ, Liv Eidsmo and Carmen Gerlach. Immunity, 2023, 56(8), 1955-1974.e10. <a href="https://doi.org/10.1016/j.immuni.2023.06.025" rel="noreferrer" target="_blank">https://doi.org/10.1016/j.immuni.2023.06.025</a></p><p dir="ltr">II. The cellular microenvironment regulates CX3CR1 expression on CD8+ T cells and the maintenance of CX3CR1+ CD8+ T cells.<b> </b><b>Jyoti Pokharel</b>*, Iman Shryki*, Anthonie Johan Zwijnenburg, Ioana Sandu, Laura Krumm, Christina Bekiari, Rebecka Heinbäck, Victor Avramov, Josefin Lysell, Liv Eidsmo, Helena Erlandsson Harris and Carmen Gerlach. *Equal contribution. European Journal of Immunology, 2024, 54(1):e2350658. <a href="https://doi.org/10.1002/eji.202350658" rel="noreferrer" target="_blank">https://doi.org/10.1002/eji.202350658</a></p><p dir="ltr">III. Preferential access to IL-15 rather than enhanced sensitivity underlies the superior self-renewal of CD8+ memory T cells with intermediate CX3CR1 expression levels.<b> </b><b>Jyoti Pokharel</b>, Wenning Zheng, Anthonie J. Zwijnenburg, Christian Sommerauer, Qirong Lin, Ulrich H. von Andrian, Claudia Kutter, Jeff E. Mold, and Carmen Gerlach. [Manuscript]</p> 134ce02e-89da-424a-906d-8a260a744776 24b6f4f4-8161-4e6a-8af4-444f038deae8 10.69622/31486840.v1 31d222b4-11e0-434b-b5ae-088119c51189 bccb3266-689d-4740-a039-c96594b4d916 baaa59e1-745b-46e3-b068-aaa3dae9eef9 bccb3266-689d-4740-a039-c96594b4d916 9c3a4abc-2005-4f50-8eff-4eb14e273cff ce30924e-799c-4b6f-b157-f6b36d802ec7 18690457 Jyoti Pokharel 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/access_right open access

oai:figshare.com:article/32124756 2026-04-29T00:00:00Z category_1 category_7 category_12 category_14 category_15 category_16 category_39 category_61 category_69 category_122 category_135 category_272 category_873 portal_63 item_type_6 month_year_04_2026

32124756 Coconut-Inspired Sandwich Three-Shell-Structured Silicon/Carbon Anode for Durable Lithium-Ion Batteries Advanced Si anodes are urgently in demand for developing high-energy-density lithium-ion batteries (LIBs). Herein, we develop an attractive coconut-like three-shell-structured Si/C composite with a Si content of 35 wt %, i.e., thin-shelled hollow carbon@Si shell@carbon shell (h-C@Si@C). The optimized h-C@Si@C material demonstrates endurable cycling performance in half-cells (886 mAh g^–1 at 2.5 A g^–1 after 500 cycles) and full cells with a LiCoO₂ cathode (131 mAh g^–1 at 1 C after 700 cycles). The enhanced electrochemical performance is ascribed to the distinctive architecture of h-C@Si@C: (i) The elastic inner carbon shell with cavities releases the lithiation-induced stress through inward expansion, thereby preventing the fracture of the outer carbon shell; (ii) the rigid outer carbon shell enables the formation of a stable solid electrolyte interphase during Si alloying/dealloying; (iii) the two sandwich-like carbon shells are conducive to the retention and electron contact of Si. This study demonstrates an intriguing structural design of Si/C anodes for advanced LIBs. 134ce02e-89da-424a-906d-8a260a744776 24b6f4f4-8161-4e6a-8af4-444f038deae8 10.1021/acsenergylett.6c00810.s001 31d222b4-11e0-434b-b5ae-088119c51189 bccb3266-689d-4740-a039-c96594b4d916 baaa59e1-745b-46e3-b068-aaa3dae9eef9 bccb3266-689d-4740-a039-c96594b4d916 9c3a4abc-2005-4f50-8eff-4eb14e273cff ce30924e-799c-4b6f-b157-f6b36d802ec7 16700702 Zirui Shu 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 56049 Xuan Zhang 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 206872 Xiaoyu Liu 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 16494040 Changkai Zhou 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 1508176 Hai Deng 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 356935 Lijun Yang 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 690293 Hongwen Huang 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 31071 Qiang Wu 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 1489591 Xizhang Wang 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 220019 Zheng Hu 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/access_right open access

oai:figshare.com:article/32124750 2026-04-29T00:00:00Z category_1 category_4 category_13 category_14 category_19 category_21 category_64 category_272 category_734 category_873 portal_63 item_type_6 month_year_04_2026

32124750 Tetrahydromethanopterin as a Two-Carbon Carrier: Formation of N⁵‑Ethyl- and N⁵,N¹⁰-Ethylene-Tetrahydromethanopterin in Methanothermobacter marburgensis Tetrahydromethanopterin (H₄MPT) is the main carbon carrier in methane metabolism, which allows the interconversion of a formyl group into a methyl group. Although typically described as a single carbon (C1) carrier, we identified and characterized <i>N</i>⁵-ethyltetrahydromethanopterin (<i>N</i>⁵-ethyl-H₄MPT) and <i>N</i>⁵,<i>N</i>¹⁰-(1,1-ethylene)tetrahydromethanopterin (<i>N</i>⁵,<i>N</i>¹⁰-ethylene-H₄MPT) in the model methanogen <i>Methanothermobacter marburgensis</i>. The chemical competence of H₄MPT to accept C2 moieties was assayed through the study of the spontaneous formation of <i>N</i>⁵,<i>N</i>¹⁰-ethylene-H₄MPT by the condensation of acetaldehyde with H₄MPT under standard conditions. The rate constant for this reaction was determined to be 1.53 ± 0.05 M^–1 s^–1, and the equilibrium constant was determined to be (8.8 ± 0.5) × 10³ M^–1, which is ca. 35 times higher compared to the analogous reaction with the more common carbon carrier tetrahydrofolate. Biochemical assays with <i>M. marburgensis</i> cell lysate suggest that the observed formation of <i>N</i>⁵-ethyl-H₄MPT relies on the enzymatic reduction of <i>N</i>⁵,<i>N</i>¹⁰-ethylene-H₄MPT. These findings illustrate the chemical competency of H₄MPT to promote biocatalysis with two-carbon moieties and highlight that such reactions might be compatible with established H₄MPT-mediated microbial pathways. 134ce02e-89da-424a-906d-8a260a744776 24b6f4f4-8161-4e6a-8af4-444f038deae8 10.1021/acs.biochem.6c00178.s001 31d222b4-11e0-434b-b5ae-088119c51189 bccb3266-689d-4740-a039-c96594b4d916 baaa59e1-745b-46e3-b068-aaa3dae9eef9 bccb3266-689d-4740-a039-c96594b4d916 9c3a4abc-2005-4f50-8eff-4eb14e273cff ce30924e-799c-4b6f-b157-f6b36d802ec7 23810643 Maxime G. Laird 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 23810646 Heta Telimaa 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 2674993 Jari Koivisto 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 1901029 Silvan Scheller 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/access_right open access

oai:figshare.com:article/32124748 2026-04-29T12:43:22Z category_133 portal_316 item_type_3 month_year_04_2026

32124748 Table 1_Selection and validation of suitable reference genes for qPCR expression analysis in young in vitro grown kohlrabi under cytokinin and sucrose treatments.docx <p>Reference genes (RGs) commonly serve as internal controls for normalization of gene expression data in quantitative real-time polymerase chain reaction (qPCR) analyses. To ensure accurate and reliable normalization and interpretation of the results, a systematic validation of RG stability under particular experimental conditions is essential. Our research group previously established a suitable in vitro model system for kohlrabi (Brassica oleracea var. gongylodes) growth and regeneration, encompassing four early developmental stages under varying cytokinin and sugar treatments. As the stability of potential RGs had not yet been evaluated for kohlrabi, we selected and tested suitable RGs for our specific experimental setup. A total of 15 candidate RGs were analyzed using the RefFinder online tool, which integrates four statistical algorithms (geNorm, NormFinder, BestKeeper, and the comparative ΔCt method) to generate a comprehensive stability ranking. TUA2, ACT7 and SAND were identified as the most stable RGs across all experimental subsets, while GAPB and GLUR3.2 exhibited the lowest stability. These findings were validated by qPCR analysis of selected sucrose-related target genes. Our results highlight the importance of context-specific RG validation and provide a valuable resource for gene expression studies in non-model horticultural crops such as kohlrabi.</p> 134ce02e-89da-424a-906d-8a260a744776 24b6f4f4-8161-4e6a-8af4-444f038deae8 10.3389/fpls.2026.1820839.s001 31d222b4-11e0-434b-b5ae-088119c51189 bccb3266-689d-4740-a039-c96594b4d916 baaa59e1-745b-46e3-b068-aaa3dae9eef9 bccb3266-689d-4740-a039-c96594b4d916 f150e5de-204e-4b24-94a6-bed814e99ddf ce30924e-799c-4b6f-b157-f6b36d802ec7 23810641 Anđela Tomić 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 23083321 Jelena Milojević 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 17881895 Martin Raspor 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 13010307 Mariana Stanišić 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 20445131 Branka Uzelac 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 17881916 Slavica Ninković 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 17881901 Tatjana Ćosić 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/access_right open access

oai:figshare.com:article/25738818 2024-05-02T15:17:23Z category_25972 portal_1083 item_type_3 month_year_05_2024

25738818 Supplementary materials: Effects of cardiovascular single pill combinations compared with identical multi-pill therapies on healthcare cost and utilization in Germany <p dir="ltr"><b>These are peer-reviewed supplementary materials for the article '</b><b>Effects of cardiovascular single pill </b><b>combinations compared with identical </b><b>multi-pill therapies on healthcare cost and </b><b>utilization in Germany</b><b>' published in the</b><b> </b><b><i>Journal of Comparative Effectiveness Research</i></b><b>.</b></p><ul><li><b>Supplementary figure 1</b></li><li><b>Supplementary figure 2</b></li><li><b>Supplementary figure 3</b></li><li><b>Supplementary table 1</b></li><li><b>Supplementary table 2</b></li></ul><p dir="ltr"><b>Aim:</b> This study assessed whether a single pill combination (SPC) is associated with lower direct healthcare costs. <b>Materials & methods:</b> Anonymized claims data of patients ≥18 years treated with drugs for cardiovascular (CV)-related diseases either as a single pill combination or multi-pill combination (follow up to 1 year) were evaluated. After propensity score matching, 59,336 out of 1,369,840 patients were analyzed.<b> Results:</b> In all cohorts, patients receiving a single pill combination had a lower frequency of general practitioner and specialist visits. The patients also had a significantly lower ratio of all-cause hospitalization days and number of CV-related prescriptions as well as all-cause prescriptions (with one exception) compared with those receiving a multi-pill combination. <b>Conclusion:</b> Direct CV-related costs were significantly lower in four out of seven comparisons, with a trend toward lower costs in the other three comparisons.</p> 134ce02e-89da-424a-906d-8a260a744776 24b6f4f4-8161-4e6a-8af4-444f038deae8 10.6084/m9.figshare.25738818.v2 31d222b4-11e0-434b-b5ae-088119c51189 bccb3266-689d-4740-a039-c96594b4d916 baaa59e1-745b-46e3-b068-aaa3dae9eef9 bccb3266-689d-4740-a039-c96594b4d916 f150e5de-204e-4b24-94a6-bed814e99ddf ce30924e-799c-4b6f-b157-f6b36d802ec7 18495199 Thomas Wilke 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 818291 Burkhard Weisser 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 8505697 Hans-Georg Predel 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 96096 Roland Schmieder 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 11239461 Sven Wassmann 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 4918288 Anton Gillessen 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 18495201 Jorg Blettenberg 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 3447677 Ulf Maywald 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 12273869 Olaf Randerath 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 18495202 Sabrina Muller 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 18495204 Michael Bohm 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/access_right open access

oai:figshare.com:article/25738098 2024-05-02T14:01:07Z category_25972 portal_1083 item_type_3 month_year_05_2024

25738098 Supplementary materials: Willingness to pay for a hypothetical malaria vaccine in Brazil: a cross-sectional study and the implications <p dir="ltr"><b>These are peer-reviewed supplementary materials for the article '</b><b>Willingness to pay for a hypothetical </b><b>malaria vaccine in Brazil: a cross-sectional </b><b>study and the implications</b><b>' published in the</b><b> </b><b><i>Journal of Comparative Effectiveness Research</i></b><b>.</b></p><ul><li><b>Previous knowledge about malaria</b></li><li><b>Information about malaria</b></li><li><b>Conference about context understanding</b></li><li><b>Opinion research</b></li><li><b>Clinical data</b></li><li><b>Exclusive to interviewer</b></li></ul><p dir="ltr"><b>Aim:</b> Malaria is an infection caused by protozoa of genus Plasmodium, considered the one associated with increasingly large outbreaks. <b>Methods:</b> A cross-sectional study was conducted with residents in the northern region of Brazil on the willingness to pay (WTP) for a hypothetical vaccine against malaria (effective protection of 80%). <b>Results: </b>Of 616 people interviewed, most interviewees were female (61%) and were employed (97%). The median individual maximum WTP for a hypothetical malaria vaccine was US$11.90 (BRL 50). <b>Conclusion: </b>The northern region of Brazil is one of the largest markets for a malaria vaccine due to its epidemiological relevance. Consequently, economic studies will be important to assist in the assessment of the potential price and value of new vaccines.</p> 134ce02e-89da-424a-906d-8a260a744776 24b6f4f4-8161-4e6a-8af4-444f038deae8 10.6084/m9.figshare.25738098.v2 31d222b4-11e0-434b-b5ae-088119c51189 bccb3266-689d-4740-a039-c96594b4d916 baaa59e1-745b-46e3-b068-aaa3dae9eef9 bccb3266-689d-4740-a039-c96594b4d916 f150e5de-204e-4b24-94a6-bed814e99ddf ce30924e-799c-4b6f-b157-f6b36d802ec7 18494611 Maria Jose Labis da Costa 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 18494614 Gesiane Nascimento 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 18494617 Thannuse Athie 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 18494620 Juliana de Sales Silva 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 17940701 Edna Afonso Reis 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 18494622 Antony Martin 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 8601984 Brian Godman 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 17940704 Isabella Piassi Dias Godoi 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/access_right open access

oai:figshare.com:article/25738089 2024-05-02T13:53:43Z category_25972 category_27103 portal_1083 item_type_3 month_year_05_2024

25738089 Supplementary data: An economic evaluation of reducing colorectal cancer surveillance intensity <p dir="ltr"><b>These are peer-reviewed supplementary materials for the article '</b><b>An economic evaluation of reducing </b><b>colorectal cancer surveillance intensity</b><b>' published in the</b><b> </b><b><i>Journal of Comparative Effectiveness Research</i></b><b>.</b></p><ul><li><b>Table 1: </b>Parameters Used in the Model</li><li><b>Table 2: </b>Distributions Used for Probabilistic Sensitivity Analysis</li></ul><p dir="ltr"><b>Aim: </b>Analyze the impact of national implementation of ‘low intensity’ post-treatment colorectal cancer surveillance compared with current practices. <b>Materials & methods:</b> Create a population-level Markov model to estimate impacts of expansion of low versus high intensity surveillance post-treatment on healthcare utilization, costs and caregiver time loss. <b>Results:</b> Shifting to low intensity colorectal cancer surveillance would reduce patient burden by 301,830 h per patient annually over 5 years. Cost reductions over 5 years were US$43.5 million for Medicare and US$4.2 million for Medicaid. Total societal cost savings equaled US$104.2 million. Conclusion: National implementation of low intensity post-treatment colorectal cancer surveillance has the potential to significantly reduce burden and costs on patients and their caregivers with no added risks to health.</p> 134ce02e-89da-424a-906d-8a260a744776 24b6f4f4-8161-4e6a-8af4-444f038deae8 10.6084/m9.figshare.25738089.v2 31d222b4-11e0-434b-b5ae-088119c51189 bccb3266-689d-4740-a039-c96594b4d916 baaa59e1-745b-46e3-b068-aaa3dae9eef9 bccb3266-689d-4740-a039-c96594b4d916 f150e5de-204e-4b24-94a6-bed814e99ddf ce30924e-799c-4b6f-b157-f6b36d802ec7 18294769 Benjamin T. Allaire 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 18494599 Robbie Skinner 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 7848650 Grant King 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 18494601 Amanda Honeycutt 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/access_right open access

oai:figshare.com:article/32124735 2026-04-29T00:00:00Z category_4 category_7 category_12 category_13 category_14 category_19 category_64 category_132 category_734 category_873 portal_63 item_type_6 month_year_04_2026

32124735 Identification of a Small Molecule as Potential Hypoxia-Selective Agents by Reducing Membrane Localization of ENO1 in Pancreatic Cancer Cells The hypoxic microenvironment of pancreatic cancer drives tumorigenesis, invasion, multidrug resistance, and poor prognosis. Rakicidin A exhibits hypoxia-selective cytotoxicity, while its stability, structure–activity relationship (SAR), and molecular target remain unclear. Here, we developed an efficient synthetic route to generate a library of 34 rakicidin A analogues. SAR analysis identified compound <b>10e</b> as the most potent derivative. Target identification revealed that <b>10e</b> covalently bound to Cys357 of enolase 1 (ENO1). Mechanistically, <b>10e</b> disrupted ENO1 membrane translocation and its interaction with c-MET, leading to suppression of the PI3K/AKT signaling pathway. <i>In vivo</i> studies demonstrated that <b>10e</b> significantly inhibited tumor growth in pancreatic cancer xenograft models. This work identifies <b>10e</b> as a novel hypoxia-selective rakicidin analogue targeting ENO1 and highlights inhibition of ENO1 membrane localization as a promising strategy for hypoxia-directed pancreatic cancer therapy. 134ce02e-89da-424a-906d-8a260a744776 24b6f4f4-8161-4e6a-8af4-444f038deae8 10.1021/acs.jmedchem.5c03550.s001 31d222b4-11e0-434b-b5ae-088119c51189 bccb3266-689d-4740-a039-c96594b4d916 baaa59e1-745b-46e3-b068-aaa3dae9eef9 bccb3266-689d-4740-a039-c96594b4d916 9c3a4abc-2005-4f50-8eff-4eb14e273cff ce30924e-799c-4b6f-b157-f6b36d802ec7 5751209 Tianyang Chen 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 10953407 Fangzhi Han 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 10953410 Guangju Liu 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 1424125 Fengyuan Zhang 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 11936597 Wanjing Feng 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 5674682 Yongping Bai 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 205007 Can Liu 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 73753 Yue Chen 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 1399702 Yahui Ding 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 168081 Quan Zhang 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/access_right open access

oai:figshare.com:article/32124732 2026-04-29T00:00:00Z category_1 category_4 category_13 category_21 category_24 category_64 category_69 category_132 category_135 category_873 portal_63 item_type_6 month_year_04_2026

32124732 Preparation and Regulation of Temperature-Sensitive Organogel for Temperature-Controlled Information Displays and Smart Temperature Labels Temperature-sensitive gels are attractive in various fields, including smart windows, information encryption, and actuators. Herein, we select ethylene glycol as the solvent and prepare a poly(4-acryloylmorpholine) (PACMO) organogel with UCST phase transition behavior via photopolymerization. This temperature-sensitive organogel, with transition temperatures adjustable between 10 and 62 °C, displays high temperature sensitivity, a narrow transition window, and excellent cycling stability. The incorporation of comonomers, cosolvents, and cross-linkers significantly affects the transition behavior of the organogel through changing the transition temperature or broadening the transition window from 2 to 25 °C. Variable-temperature infrared spectroscopy and theory calculations reveal that the ether bond of PACMO competes with the intermolecular hydrogen bonds of ethylene glycol. As the temperature rises, the intermolecular hydrogen-bond interaction between ethylene glycol molecules weakens, while the hydrogen-bond interaction between the ether bond and ethylene glycol strengthens, leading to changes in transparency of the organogel. The applications of the organogel in the fields of temperature-controlled information displays and smart temperature labels are demonstrated. This design broadens the types of temperature-sensitive gel materials to organogels, opening a pathway toward applications with multifunctional optical requirements. 134ce02e-89da-424a-906d-8a260a744776 24b6f4f4-8161-4e6a-8af4-444f038deae8 10.1021/acsami.6c01830.s001 31d222b4-11e0-434b-b5ae-088119c51189 bccb3266-689d-4740-a039-c96594b4d916 baaa59e1-745b-46e3-b068-aaa3dae9eef9 bccb3266-689d-4740-a039-c96594b4d916 9c3a4abc-2005-4f50-8eff-4eb14e273cff ce30924e-799c-4b6f-b157-f6b36d802ec7 378690 Yang Hou 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 8567241 Guoqiang Lu 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 11276070 Liangyu He 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 488239 Jun Nie 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 764023 Xiaoqun Zhu 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/access_right open access

oai:figshare.com:article/32124730 2026-04-29T12:40:13Z category_393 portal_316 item_type_1 month_year_04_2026

32124730 Image 1_Resident T cell activation leads to human hair follicle immune privilege loss ex vivo, which is prevented by the DHODH inhibitor farudodstat: relevance for alopecia areata.pdf <p>The autoimmune hair loss disorder alopecia areata (AA), is characterized by immune privilege (IP) collapse of the hair follicle (HF) bulb resulting from a Th1-dependent inflammatory response. Although CD8⁺ T cells are recognized key drivers of the disease, it remains to be clarified whether the activation of HF resident T cells suffice to initiate IP loss and thus elicit the cascade of events leading to AA. Here, we utilized the human microdissected HF organ culture model to answer this question by activating intra- and peri-follicular HF resident T cells with αCD3/αCD28 antibodies. TCR stimulation indeed resulted in enhanced resident T cell proliferation, as indicated by significantly increased CD3⁺Ki-67⁺ cells, and higher intrafollicular CD3⁺ T cell numbers. Furthermore, αCD3/αCD28 stimulation promoted key signs of HF IP collapse, by increasing bulbar MHC class I and II expression and elevating MHC class II⁺ cells numbers. We next sought to investigate whether T cell proliferation plays a central role in the TCR activation–dependent collapse of the bulb IP. To test this, we co-administered the DHODH inhibitor farudodstat 1 day prior and during the stimulation with αCD3/αCD28 in HF organ culture. Short-term treatment with farudodstat reduced the increase in T cell proliferation and significantly decreased the upregulated MHC class I and II expression induced by TCR stimulation with αCD3/αCD28. Our results show that stimulation of HF resident T cells via TCR engagement induces an AA–like phenotype in healthy human HFs ex vivo, characterized by T cell proliferation and subsequent IP collapse. DHODH inhibition with farudodstat only partially reduces T cell numbers but prevented HF IP collapse induction.</p> 134ce02e-89da-424a-906d-8a260a744776 24b6f4f4-8161-4e6a-8af4-444f038deae8 10.3389/fmed.2026.1810644.s001 31d222b4-11e0-434b-b5ae-088119c51189 bccb3266-689d-4740-a039-c96594b4d916 baaa59e1-745b-46e3-b068-aaa3dae9eef9 bccb3266-689d-4740-a039-c96594b4d916 20947cb9-79ae-47c5-8595-982626dd010f ce30924e-799c-4b6f-b157-f6b36d802ec7 33003 Ilaria Piccini 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 23810617 Thomas Rouillé 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 405807 Wolfgang Funk 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 8017871 Francisco Jimenez 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 23810620 Alexandre Kaoukhov 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 23810623 Carl Firth 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 359650 Amos Gilhar 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 23810626 Janin Edelkamp 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 23810629 Ferda Cevikbas 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 564966 Marta Bertolini 60f2a090-1cfe-11e1-8bc2-0800200c9a66 b7135ad0-1d00-11e1-8bc2-0800200c9a66 http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/access_right open access eyJtZXRhZGF0YVByZWZpeCI6ICJjZXJpZiIsICJ2ZXJiIjogIkxpc3RSZWNvcmRzIiwgIm9haV9oYW5kbGVyIjogdHJ1ZSwgInBhZ2UiOiAyLCAieEN1cnNvciI6ICJleUpoYkdjaU9pSklVelV4TWlKOS5JbHN4TnpjM05EWTJOREV6TURBd0xDQXpNakV5TkRjek1GMGkuUjQtYUJ5TTdTekdyRlVWajloaUwxbWg3UzRKSFF1elNsLWtHc2hMOEFKeVpKSUFPVm5lTW5zUXZhUkxaNXI2ZUZyNzNOLWxGYlFXRzRQazdkUjhDNHciLCAiZXhwaXJhdGlvbiI6ICIyMDI2LTA0LTI5IDEzOjQ1OjI3In0=