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oai:figshare.com:article/34512922 2026-10-14T17:26:03Z category_8 category_272 category_39 category_734 category_69 portal_5 item_type_3 month_year_10_2026

<p>18S ONT tagged primers. Primers amplifying 1263 bp of the 18S rRNA gene (shown in purple) were modified with 8mer barcodes (represented in blue) for identifying the three replicates a, b, and c for each site. The Oxford Nanopore Technologies sequencing tags (depicted in red) were added from the PCR Barcoding Kit (SQK-BK004) according to their Four-Primer PCR protocol.</p> Justin Gibbons (3495656) Heidi Pagán (5586998) Tatiana Ferrer (4993826) Rays H. Y. Jiang (10339115) Greg O’Corry-Crowe (4993835) Microbiology Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry overlook eukaryotic bloom indian river lagoon highlighting clear differences harmful algal blooms eukaryotic microbial communities capture eukaryotic diversity 18s rrna gene pyrodinium bahamense </ karlodinium veneficum </ sequencing yielded high resolution community profiles 200 unique species aureoumbra lagunensis </ read sequencing targeting div >< p lagunensis </ read sequencing community composition hab species forming species water samples temperate subregions seasonal patterns results show relative abundance promising tool nutrient ratios near real irl ), characterize spatial atlantic coast approach offers <p>18S ONT tagged primers. Primers amplifying 1263 bp of the 18S rRNA gene (shown in purple) were modified with 8mer barcodes (represented in blue) for identifying the three replicates a, b, and c for each site. The Oxford Nanopore Technologies sequencing tags (depicted in red) were added from the PCR Barcoding Kit (SQK-BK004) according to their Four-Primer PCR protocol.</p> 2026-10-14T17:25:45Z Dataset Dataset 10.1371/journal.pwat.0000557.t001 https://figshare.com/articles/dataset/_p_18S_ONT_tagged_primers_Primers_amplifying_1263_bp_of_the_18S_rRNA_gene_shown_in_purple_were_modified_with_8mer_barcodes_represented_in_blue_for_identifying_the_three_replicates_a_b_and_c_for_each_site_The_Oxford_Nanopore_Technologies_seq/34512922 CC BY 4.0