2026-10-06T06:16:18Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34074533 2026-10-05T18:05:11Z category_1 category_4 category_146 category_7 category_12 category_15 category_16 category_21 category_873 category_106 portal_63 item_type_2 month_year_10_2026

Single-Objective Light-Sheet Microscopy Allows Long-Term, High-Resolution In Vivo Brain Imaging of Drosophila Francisco J. Tassara (25317452) Mariano Barella (9437269) Tomás Sebastián Fernández Troncoso (25317455) Lourdes Simó (25317458) M. Mailén Folgueira Serrao (25317461) Micaela Rodriguez-Caron (22976230) Juan Ignacio Ispizua (14294845) Mark H. Ellisman (1225158) Horacio O. de la Iglesia (9459129) M. Fernanda Ceriani (24893731) Julián Gargiulo (1394827) Biophysics Biochemistry Space Science Medicine Cell Biology Neuroscience Physiology Biotechnology Chemical Sciences not elsewhere classified Science Policy understanding several phenomena revealing neuronal plasticity features enabled time dynamic mitochondrial transport sheet microscope customized setup complexity required among spatial resolution intact adult flies high spatial resolution lapse brain imaging vivo </ italic adult flies vivo </ sheet technologies mounting complexity small brain living flies maximum resolution brain imaging without relying present drososcope plasma membrane objective light lower invasiveness fruit fly drift correction core vesicles confocal microscopy axonal projections alternative light 5 h 310 nm <i>In vivo</i> imaging of dynamic subcellular brain structures is key to understanding several phenomena in neuroscience. However, in the fruit fly, its implementation has been hindered by a trade-off among spatial resolution, speed, photobleaching, phototoxicity, and setup complexity required to access specific target regions of the small brain of Drosophila. Here, we present DrosoScope: a single-objective light-sheet microscope customized for <i>in vivo</i> imaging of adult flies and optimized for maximum resolution. With it, we imaged the axonal projections of small lateral ventral neurons (s-LNvs) in intact adult flies. We imaged the plasma membrane, cytoplasm, mitochondria, and dense-core vesicles with high spatial resolution of up to 310 nm, lower photobleaching than confocal microscopy, lower invasiveness and reduced sample-mounting complexity than alternative light-sheet technologies, and without relying on potentially phototoxic pulsed infrared lasers. Furthermore, we implemented active focus stabilization and sample-drift correction. Combined, these features enabled time-lapse brain imaging in living flies for up to 5 h, revealing neuronal plasticity and dynamic mitochondrial transport. 2026-10-05T00:00:00Z Dataset Media 10.1021/acsphotonics.6c01278.s004 https://figshare.com/articles/media/Single-Objective_Light-Sheet_Microscopy_Allows_Long-Term_High-Resolution_In_Vivo_Brain_Imaging_of_Drosophila/34074533 CC BY-NC 4.0