2026-10-06T05:21:24Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34074251 2026-10-05T17:58:10Z category_4 category_8 category_12 category_13 category_14 category_15 category_46 category_734 category_61 category_132 portal_5 item_type_3 month_year_10_2026

<p>Aversion protocol.</p> Romane Milleville (25317389) Gérard Manière (54979) Martine Berthelot-Grosjean (19867) Olivier Lamotte (211957) Yaël Grosjean (17616307) Julien Royet (201408) C. Léopold Kurz (25317392) Biochemistry Microbiology Cell Biology Genetics Molecular Biology Neuroscience Immunology Biological Sciences not elsewhere classified Developmental Biology Infectious Diseases receptor proteins encoded reactive oxygen species internal physiological conditions defined neuronal circuits signal remained unknown bacterial peptidoglycan recognition bacterial metabolite responsible b neuron activation results identify uracil findings reveal uracil larval terminal organ certain pathogenic bacteria driven ros signaling bacterial signal ros signaling expressing neuron bacterial cue larval gut systems rely specific expression sensory systems previously shown molecules involved immediate environment host integration host cells gustatory response exogenous factors drosophila </ dependent production <div><p>Animals perceive their immediate environment through their sensory systems. While these systems rely on well-defined neuronal circuits and receptor proteins encoded by the genome, they can also be modulated by internal physiological conditions or exogenous factors. We have previously shown that the presence of certain pathogenic bacteria in the larval gut can alter the specificity of bacterial peptidoglycan recognition in the adults that emerge from these larvae. However, the nature of the bacterial signal and the host cells and molecules involved in receiving and transducing this signal remained unknown. Our results identify uracil as the bacterial metabolite responsible for this priming and demonstrate that the effect is mediated by Duox-dependent production of reactive oxygen species (ROS) in the larval gut. We further show that specific expression of TrpA1 isoforms in a Gr66a-expressing neuron of the larval terminal organ is required for the adult response. Together, these findings reveal uracil as the bacterial cue and the Duo/ROS signaling and TrpA1/Gr66a modules as key mediators linking larval gut microbial signals and host integration to subsequent sensory system modulation in the adult.</p></div> 2026-10-05T17:57:46Z Dataset Dataset 10.1371/journal.pbio.3004042.t001 https://figshare.com/articles/dataset/_p_Aversion_protocol_p_/34074251 CC BY 4.0