2026-10-12T18:36:16Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34074152 2026-10-05T17:57:30Z category_4 category_13 category_14 category_21 category_24 category_734 category_931 category_64 category_132 category_134 portal_5 item_type_3 month_year_10_2026

<p>Evaluation of Phred score calibration methods on variant calling accuracy on mixed plasmid datasets (R9.4.1), comparison of scalar reduction versus curve-fitting approaches (quadratic, polynomial, Gaussian) derived from QvQ plots of clonal plasmids. Only scalar methods resulted in precisions of 100% while scalar minus 5 provided optimal balance between precision and recall (F1: 76.2).</p> Xavier Zair (25317383) Andreas Wilm (7951) Miles C. Benton (9601012) Cheng Yong Tham (8528523) Lin Yang (45852) Paola Florez de Sessions (9048782) October Michael Sessions (6635093) Eng Hui Chew (25317386) Swapnil Mishra (8611860) Biochemistry Genetics Molecular Biology Biotechnology Evolutionary Biology Biological Sciences not elsewhere classified Information Systems not elsewhere classified Cancer Infectious Diseases Virology widely used caller tracking viral evolution sequenced using r9 oxford nanopore technologies false discovery rates benchmarked across chemistries consensus variant calling lofreq &# 8217 xlink "> next 1 flow cells xlink "> lofreq xlink "> consensus variants study assesses short reads library sizes less effective haploid genomes generation sequencing effective sequencing detecting variants best performance also introduced 7179 bases 100 snvs <p>Evaluation of Phred score calibration methods on variant calling accuracy on mixed plasmid datasets (R9.4.1), comparison of scalar reduction versus curve-fitting approaches (quadratic, polynomial, Gaussian) derived from QvQ plots of clonal plasmids. Only scalar methods resulted in precisions of 100% while scalar minus 5 provided optimal balance between precision and recall (F1: 76.2).</p> 2026-10-05T17:57:18Z Dataset Dataset 10.1371/journal.pone.0356259.t002 https://figshare.com/articles/dataset/_p_Evaluation_of_Phred_score_calibration_methods_on_variant_calling_accuracy_on_mixed_plasmid_datasets_R9_4_1_comparison_of_scalar_reduction_versus_curve-fitting_approaches_quadratic_polynomial_Gaussian_derived_from_QvQ_plots_of_clonal_plas/34074152 CC BY 4.0