2026-10-13T05:34:36Z
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oai:figshare.com:article/34073877
2026-10-05T17:53:43Z
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<p>Oligonucleotides used for directed mutagenesis and heterologous expression of TRX <i>h1</i> in <i>Escherichia coli</i>. Restriction sites are underlined (PeahN, NotI; PeahC, BamHI).</p>
Ewelina Ratajczak (5337173)
Aleksandra M. Staszak (25317300)
Paola Vargas (19314397)
Mariam Sahrawy (3537725)
Antonio J. Serrato (25317303)
Hanna Fuchs (25317306)
Biochemistry
Genetics
Biotechnology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
western blotting confirmed
l .): insights
genetic information processing
functional categories related
div >< p
9 ± 1
5 vs 45
0 ± 0
european beech (<
reduced thiol pool
target capture proceeded
gradually lose viability
>- targeted proteins
fagus sylvatica </
changing environmental conditions
thiol redox status
beech embryonic axes
term stored seeds
improving seed storage
beech seeds
target proteins
embryonic axes
seed viability
cytosolic thiol
binding proteins
seed physiology
h1 </
tricarboxylic acid
trapping mutant
total thiols
tagged monocysteinic
superoxide dismutase
stress response
results suggest
reducible mixed
redox homeostasis
protein stability
protein homeostasis
oxidative stress
metabolic control
glutathione peroxidase
fold lower
<p>Oligonucleotides used for directed mutagenesis and heterologous expression of TRX <i>h1</i> in <i>Escherichia coli</i>. Restriction sites are underlined (PeahN, NotI; PeahC, BamHI).</p>
2026-10-05T17:53:34Z
Dataset
Dataset
10.1371/journal.pone.0359622.t001
https://figshare.com/articles/dataset/_p_Oligonucleotides_used_for_directed_mutagenesis_and_heterologous_expression_of_TRX_i_h1_i_in_i_Escherichia_coli_i_Restriction_sites_are_underlined_PeahN_NotI_PeahC_BamHI_p_/34073877
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