2026-10-07T04:33:39Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34073377 2026-10-05T17:47:34Z category_1 category_4 category_12 category_13 category_14 category_734 portal_5 item_type_3 month_year_10_2026

<p>List of H3K27me3-marked genes used in analysis \.</p> Rochelle E. Yap (13184347) Felicia Ebot-Ojong (5783645) Abigail J. Ameri-Solanky (25317211) Zachary A. Lewis (11172108) Biophysics Biochemistry Cell Biology Genetics Molecular Biology Biological Sciences not elsewhere classified neurospora crassa </ histone acetyltransferase rtt109 although rtt109 interacts div >< p specific prc2 targeting establish rtt109 stably propagated repressed state repressed domains proper structure multiple rounds model fungus linked mechanism key regulator h3k56 acetylation findings uncover epigenetic memory catalyzes trimethylation <div><p>In animals, plants, and some fungi, Polycomb Repressive Complex 2 (PRC2) catalyzes trimethylation of histone H3 lysine 27 (H3K27me3) to establish transcriptionally repressed chromatin. Here, we identify the histone acetyltransferase RTT109 as a key regulator of PRC2-repressed domains in the model fungus <i>Neurospora crassa</i>. Although RTT109 interacts with the Vps75 homolog Nucleosome Assembly Factor 2 (NAF-2), we show that proper structure and function of PRC2-methylated chromatin require RTT109 catalytic activity but are independent of NAF-2 and H3K56 acetylation. We further demonstrate that H3K27me3 can be stably propagated over multiple rounds of mitosis in the absence of sequence-specific PRC2 targeting, and that RTT109 is essential for maintenance of the repressed state. These findings uncover a replication-linked mechanism for epigenetic memory and establish RTT109 as a key regulator of Polycomb-mediated chromatin inheritance.</p></div> 2026-10-05T17:47:13Z Dataset Dataset 10.1371/journal.pgen.1012327.s013 https://figshare.com/articles/dataset/_p_List_of_H3K27me3-marked_genes_used_in_analysis_p_/34073377 CC BY 4.0