2026-10-07T23:20:45Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34070973 2026-10-05T17:04:41Z category_24307 category_24310 category_24295 item_type_3 month_year_10_2026

HiC and Capture-HiC data Hengchao Zhang (25316646) Genome structure and regulation Genomics Epigenetics (incl. genome methylation and epigenomics) Epigenetics Erythropoiesis Gene expression Research Methodology Hematopoietic lineage development <p dir="ltr">Pioneer transcription factors specify cell identity by targeting silent chromatin and initiating lineage-specific programming, yet their roles in human erythropoiesis remain incompletely defined. Here, through integrated multi-omics analysis, we identify TAL1 as a bona fide pioneer transcription factor that coordinates a multi-layered chromatin remodeling program essential for erythroid commitment. We demonstrate that TAL1 binds nucleosomal DNA and localizes predominantly to closed chromatin regions. Mechanistically, TAL1 recruits the SWI/SNF chromatin remodeling complex via direct interaction with BRG1, enabling accessibility at target loci. At the level of 3D genome organization, TAL1 establishes lineage-specific chromatin loops, and its depletion disrupts long-range interactions, leading to loss of erythroid identity and aberrant activation of myeloid programs. Finally, we delineate a downstream effector pathway wherein TAL1 directly activates the chromatin remodeler BPTF and the histone modifier KMT2A, which execute complementary functions-BPTF safeguards progenitor survival while KMT2A enforces differentiation commitment. Our findings establish a hierarchical model of erythropoiesis orchestrated by TAL1 pioneer activity, revealing how a single transcription factor coordinates chromatin remodeling across multiple levels to enforce lineage commitment.</p> 2026-10-05T17:04:41Z Dataset Dataset 10.6084/m9.figshare.34070973.v1 https://figshare.com/articles/dataset/HiC_and_Capture-HiC_data/34070973 CC BY 4.0