2026-10-09T00:38:07Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34055921 2026-10-02T09:00:09Z category_1 category_4 category_12 category_13 category_14 category_16 category_734 category_61 category_64 category_69 portal_87 item_type_3 month_year_10_2026

Evaluating the functional involvement of <i>CALM</i> and <i>ATP2B</i> in 5-hydroxytryptamine-induced larval metamorphosis in <i>Mytilus coruscus</i> Changyu Guo (23771338) Jiajie Xu (428625) Xiuping Yang (478838) Deborah M. Power (8864297) Xiao Liang (185891) Lihua Peng (2285215) Jin-Long Yang (417341) Biophysics Biochemistry Cell Biology Genetics Molecular Biology Physiology Biological Sciences not elsewhere classified Developmental Biology Cancer Inorganic Chemistry Mytilus coruscus 5-HT larval metamorphosis CALM ATP2B Ca2+ <p>Larval settlement and metamorphosis are critical developmental transitions in the macrofouling organisms, directly impacting population recruitment. Although 5-hydroxytryptamine (5-HT) effectively induces these processes, the downstream molecular mechanisms, particularly the role of intracellular Ca²⁺ homeostasis, in larval settlement and metamorphosis of <i>Mytilus coruscus</i> remain poorly understood. In this study, we investigated the roles of the calmodulin gene (<i>CALM</i>) and the plasma membrane Ca²⁺-ATPase gene (<i>ATP2B</i>), in 5-HT induced larval settlement and metamorphosis. Compared to pediveliger larvae, the expression of both <i>CALM</i> and <i>ATP2B</i> showed significant differences after metamorphosis. In 5-HT exposed larvae, <i>CALM</i> and <i>ATP2B</i> were up-regulated by 41.63% and 33.52%, respectively, whereas a <i>CALM</i> knockdown subsequently down-regulated <i>CALM</i> and up-regulated <i>ATP2B</i>. Knockdown of the <i>CALM</i> gene significantly inhibited the inductive effects of 5-HT on larval settlement and metamorphosis, with a similar reduction by <i>ATP2B</i> knockdown. The 5-HT-induced Ca²⁺ fluctuations were resolved over time in <i>ATP2B</i>-deficient larvae, but persisted in <i>CALM</i>-deficient larvae. Collectively, our findings identify <i>CALM</i> as the key mediator of 5-HT-induced larval metamorphosis by reciprocally regulating <i>ATP2B</i> and Ca²⁺ homeostasis. This knowledge of larval metamorphosis regulation is crucial for explaining macrofouling settlement and for advancing biofouling control technologies by providing new targets.</p> 2026-10-02T09:00:09Z Dataset Dataset 10.6084/m9.figshare.34055921.v1 https://figshare.com/articles/dataset/Evaluating_the_functional_involvement_of_i_CALM_i_and_i_ATP2B_i_in_5-hydroxytryptamine-induced_larval_metamorphosis_in_i_Mytilus_coruscus_i_/34055921 CC BY 4.0