2026-10-06T04:18:13Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34054890 2026-10-02T06:08:11Z category_24436 category_24433 item_type_3 month_year_10_2026

Data from laboratory experiments investigating the effects of environmental calcium and magnesium concentrations, and salinity, on post-moult calcium and acid-base fluxes in whiteleg shrimp (<i>Penaeus vannamei</i>). Trystan Sanders (22069133) Invertebrate biology Comparative physiology aquaculture acid-base physiology crustacean calcification <p dir="ltr">Two separate experiments were conducted to investigate how seawater [Ca²⁺] and [Mg²⁺] (at a constant Ca:Mg of 1:5) impact ion and acid bases fluxes during post-moult calcification in juvenile whiteleg shrimp. Both experiments were conducted in 2023 at the Aquatic Resources Centre at the University of Exeter. Shrimp (n = 30; wet mass = 0.57 ± 0.03 g) in Experiment 1 were acutely exposed (24 h) to a range of [Ca²⁺] (1.8 – 25 mmol/L) and [Mg²⁺] (10 – 119 mmol/L) levels at a constant Ca:Mg of 1:5 with all other major ions (Na⁺, Cl^-, K⁺) kept constant. In Experiment 2, shrimp (n = 54; wet mass = 1.13 ± 0.03 g) were acclimated for 4 – 10 days to progressively lower seawater salinities (25, 20, 15, 10, 5, 2.5 and 1 ppt) under either naturally diluted or artificially enriched Ca²⁺ + Mg²⁺ seawater treatments. The aim of Exp. 1 was to characterise the relationship between divalent cation availability and Ca²⁺ uptake, while Exp. 2 aimed to assess the seawater salinity below which Ca²⁺ uptake is impaired, and whether this is a result of low Ca²⁺ + Mg²⁺ availability. In both experiments, whole-body Ca²⁺ and acid-base (HCO₃^-and NH₄⁺) fluxes were measured in isolated flux chambers in post-moult individuals over a 24 h period with replicates corresponding to individual 24 h flux incubations. Fluxes were calculated by measuring the change in flux chamber water concentrations of ions over time and corrected for animal wet mass and measurement period. In experiment 2 only, moulted exoskeletons (exuvia) underwent compositional analysis to determine content of chitin, protein and mineral as well as the Ca and Mg content of exoskeleton mineral.</p> 2026-10-02T06:08:11Z Dataset Dataset 10.6084/m9.figshare.34054890.v1 https://figshare.com/articles/dataset/Data_from_laboratory_experiments_investigating_the_effects_of_environmental_calcium_and_magnesium_concentrations_and_salinity_on_post-moult_calcium_and_acid-base_fluxes_in_whiteleg_shrimp_i_Penaeus_vannamei_i_/34054890 CC BY 4.0 Open Access after 2027-02-02