2026-10-07T19:11:01Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34054803 2026-10-02T05:42:29Z category_19 portal_316 item_type_3 month_year_10_2026

Data Sheet 1_Wild ginseng adventitious roots restore sleep homeostasis in sleep-deprived Drosophila melanogaster.docx Hong Chen (108084) Xiangzhu Li (11462269) Hang Chi (1499950) Yongjun Zheng (1660624) Yingna Li (21171353) Lu Xing (2551372) Rui Ma (239028) Liwei Sun (109973) Xuenan Chen (13218345) Pharmacology cellular homeostasis Drosophila melanogaster ginseng adventitious roots sleep deprivation sleep regulation Introduction<p>Sleep deprivation is increasingly recognized as a major health concern and is associated with impaired physiological and neurological functions. Panax ginseng C. A. Mey. has long been used to improve sleep quality; however, the limited availability and long cultivation cycle of natural ginseng restrict its sustainable utilization. Wild ginseng adventitious roots (ADV), produced via plant tissue culture, provide a renewable source of bioactive ginsenosides, although their potential role in sleep regulation remains poorly understood.</p>Methods<p>The sleep-promoting effects and safety of ADV were evaluated in a sleep deprivation-induced Drosophila melanogaster model. Sleep parameters, including total sleep duration, sleep latency, deep sleep, and sleep fragmentation, were assessed, together with addiction-like behavioral assays. Transcriptomic analysis was performed to investigate the underlying mechanisms. LC-MS/MS and bioactivity assays were conducted to identify and validate major active ginsenosides.</p>Results<p>ADV significantly improved sleep behavior in sleep-deprived flies, increasing total sleep duration and deep sleep while reducing sleep latency and sleep fragmentation, without inducing addiction-like behaviors. Transcriptomic analysis revealed that ADV modulated multiple sleep-related biological processes, particularly stress response pathway, as evidenced by altered expression of Hsp68, Hsp70Aa, Hsp70Ba, Hsp70Bb, and Hsp70Bbb. Combined with LC-MS/MS identification and biological activity comparison, ginsenosides Rc and F2 were preliminarily identified as the principal active metabolites.</p>Conclusion<p>In the Drosophila melanogaster sleep-deprivation model, ADV treatment is associated with improvements in sleep-related behavioural parameters alongside altered expression of conserved stress-response-related biological processes. These findings support ADV as a sustainable source of bioactive ginsenosides, though the current experimental design cannot establish a strictly sleep-specific or causal mechanistic relationship.</p> 2026-10-02T05:42:29Z Dataset Dataset 10.3389/fphar.2026.1907250.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Wild_ginseng_adventitious_roots_restore_sleep_homeostasis_in_sleep-deprived_Drosophila_melanogaster_docx/34054803 CC BY 4.0