2026-10-06T11:07:24Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34054281 2026-10-02T06:40:21Z category_24193 category_24199 category_23962 item_type_3 month_year_10_2026

Processed data for: Lycium chinense leaf polysaccharide (LCP) modulates hepatic gluconeogenesis and reshapes gut microbiota in juvenile largemouth bass (Micropterus salmoides) under a high-starch diet Junde Fan (25162266) Genomics and transcriptomics Sequence analysis Aquaculture Micropterus salmoides Lycium chinense Polysaccharides -- Therapeutic use transcriptome analyses suggested gut microbiota 3 16S rRNA cDNA gluconeogenesis-related pi3k-akt-dependent PICRUSt2 analysis Aquaculture animal nutrition <p dir="ltr">This dataset provides the processed data supporting the manuscript"LCP modulates hepatic gluconeogenesis and gut microbiota in juvenileMicropterus salmoides (largemouth bass)" (submitted to the EgyptianJournal of Aquatic Research). It contains eight summary tablesextracted from the doctoral dissertation (Fan, UMS 2026) and, fromVersion 2, four full-resolution matrices generated directly from theoriginal bioinformatics outputs.Experimental design: Juvenile largemouth bass were fed a low-starchcontrol (LS0, 10% starch) or high-starch diets (HS0, 20% starch, 0mg/kg LCP) supplemented with Lycium chinense leaf polysaccharides(LCP) at 300 (HS3), 900 (HS9), or 1500 (HS15) mg/kg; n = 3 fish pergroup. Tissues analysed: liver (RNA-seq transcriptomics) and gutcontents (16S rRNA amplicon sequencing).Summary tables (01–08):01_DEG_key_carb_genes_Table5.17.csv — key carbohydrate-metabolismgene DEGs (Table 5.17).02_KEGG_47gene_enrichment_Table5.13.csv — KEGG enrichment of 47sugar-metabolism genes (Table 5.13).03_phylum_relative_abundance_Table6.1.csv — phylum-level relativeabundance (%) (Table 6.1).04_genus_relative_abundance_Table6.2.csv — genus-level relativeabundance (%) (Table 6.2).05_alpha_diversity_Table6.3.csv — alpha-diversity indices (Table 6.3).06_PERMANOVA_beta_Table6.4.csv — beta-diversity PERMANOVA (Table 6.4).07_unique_shared_OTUs_Table6.5.csv — unique/shared OTU counts (Table 6.5).08_PICRUSt2_pathway_Table6.6.csv — PICRUSt2 pathway prediction (Table 6.6).Full-resolution matrices (added in Version 2):M1_DEG_all_genes_DESeq2.csv — full DESeq2 output, 58,777 genes(baseMean + three pairwise contrasts: HS0_vs_LS0, HS3_vs_LS0,HS3_vs_HS0, each with log2FC, padj, regulation). log2FC usesthe (HS − LS) convention (negative = down-regulated).M2_OTU_relative_abundance_full.csv — complete OTU relative-abundancematrix, 4,657 OTUs × 15 samples (each sample sums to 100%).M3_beta_diversity_BrayCurtis_matrix.csv — 15 × 15 symmetricBray–Curtis distance matrix (diagonal = 0).M4_PICRUSt2_KO_abundance_full.csv — full PICRUSt2 KO table,7,688 KOs × 5 groups (each group sums to 1.0).Methods notes: OTU calling via UPARSE; taxonomy assigned against theSILVA 138 database; relative abundance expressed as mean ± SD (n = 3);alpha diversity compared by Kruskal–Wallis test; beta diversity byPERMANOVA (999 permutations) on Bray–Curtis dissimilarity; DEGs calledby DESeq2 at |log2FC| ≥ 1 and P < 0.05 (padj = BH-adjusted); PICRUSt2functional prediction from 16S rRNA, two-sided t-test at P < 0.05.Raw sequencing reads are not publicly deposited because the targetjournal's raw-sequence deposition policy was to be confirmed atsubmission; this processed dataset provides the complete analyticaloutputs required to reproduce the reported results.</p><h4 dir="ltr">License: CC-BY 4.0.</h4><p></p> 2026-10-02T06:40:21Z Dataset Dataset 10.6084/m9.figshare.34054281.v2 https://figshare.com/articles/dataset/Processed_data_for_Lycium_chinense_leaf_polysaccharide_LCP_modulates_hepatic_gluconeogenesis_and_reshapes_gut_microbiota_in_juvenile_largemouth_bass_Micropterus_salmoides_under_a_high-starch_diet/34054281 CC BY 4.0