2026-10-14T08:34:12Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34053288 2026-10-02T04:35:20Z category_696 portal_316 item_type_3 month_year_10_2026

Data Sheet 1_Chronopharmacological effects of 5-aminosalicylic acid in the treatment of experimental colitis in mice.pdf Yulin Wen (25161123) Shuangye Li (14599297) Jincan Jian (25161126) Jiahui Liu (724342) Shuai Wang (109515) Haomeng Wu (7436744) Genetic Immunology 5-aminosalicylic acid experimental colitis circadian clock BMAL1 FTH1 Background<p>5-Aminosalicylic acid (5-ASA) is a first-line therapy for ulcerative colitis, but whether dosing timing influences its therapeutic efficacy remains unclear. This study aims to investigate the chronopharmacological effects of 5-ASA in experimental colitis.</p>Methods<p>A dextran sulfate sodium (DSS)-induced colitis model was used to compare the therapeutic effects of 5-ASA administered at different Zeitgeber times. Colonic proteomic profiling was performed to identify circadian-responsive proteins associated with differential drug efficacy. Intestinal epithelial-specific Bmal1 knockout mice were used to determine whether the circadian clock regulates the chronopharmacological response to 5-ASA.</p>Results<p>5-ASA showed marked time-dependent efficacy in DSS-induced colitis, with greater protection at ZT14 than at ZT2. Proteomic analysis identified ferritin heavy chain 1 (FTH1) as a circadian-responsive protein associated with differential treatment outcomes. 5-ASA suppressed FTH1 expression, whereas FTH1 overexpression attenuated anti-inflammatory effects of 5-ASA. Moreover, intestinal epithelial-specific deletion of Bmal1 largely abolished diurnal variation in both FTH1 expression and 5-ASA efficacy, indicating that the intestinal clock coordinates the time-dependent response to 5-ASA.</p>Conclusion<p>These findings identify FTH1 as a circadian-responsive factor linked to the time-dependent efficacy of 5-ASA and support circadian-guided optimization of 5-ASA administration as a potential strategy for improving UC treatment.</p> 2026-10-02T04:35:20Z Dataset Dataset 10.3389/fimmu.2026.1936952.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Chronopharmacological_effects_of_5-aminosalicylic_acid_in_the_treatment_of_experimental_colitis_in_mice_pdf/34053288 CC BY 4.0