2026-10-14T12:54:30Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34051206 2026-10-02T02:28:25Z category_24670 item_type_3 month_year_10_2026

<b>Reconfiguring the split guide/target active TtAgo for non-amplified multiplex detection of tumor nucleic acids and protein biomarkers</b> jun zhang (25160484) Medical biotechnology diagnostics (incl. biosensors) Split guide/target Amplification-free Activity regulation Molecular gating Nucleic acid and protein detection <p dir="ltr"><b>We established an optimized TtAgo catalytic mechanism and systematically defined guide DNA- and target length-dependent activity thresholds Based on these thresholds, we proposed two complementary molecular switch strategies</b><b>—</b><b>split-guide activation and split-target activation. In this strategy, otherwise catalytically inert nucleic acid fragments are reconstituted into functional TtAgo components only in the presence of the target. Consequently, the target directly participates in forming the active guide or target structure, transducing target recognition into programmable TtAgo cleavage signals without the need for nucleic acid amplification. Furthermore, we introduced FEN1-mediated molecular cleavage to accommodate heterogeneous DNA/RNA termini, and extended the split-guide concept to protein-bound enzymatic biomarkers by designing guide sequences containing apurinic/apyrimidinic (AP) sites that are activated upon APE1-mediated cleavage.</b></p> 2026-10-02T02:28:25Z Dataset Dataset 10.6084/m9.figshare.34051206.v1 https://figshare.com/articles/dataset/_b_Reconfiguring_the_split_guide_target_active_TtAgo_for_non-amplified_multiplex_detection_of_tumor_nucleic_acids_and_protein_biomarkers_b_/34051206 CC BY 4.0