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<p>Significantly lower IgG-SC numbers in hospitalized patients compared to subclinical cases.</p>
Amandine Trouchet (25157966)
Sokchea Lay (9857198)
Matteo Broketa (19690885)
Pablo Canales-Herrerias (25157969)
Angga Perima (19690879)
Bruno Iannascoli (17818357)
Sotheary Sann (12039614)
Borita Heng (25157972)
Sowath Ly (160224)
Veasna Duong (106893)
Gaël A. Millot (25157975)
Laura Walker (4841532)
Patrick England (28458)
Giovanna Barba-Spaeth (12066108)
Tineke Cantaert (68002)
Pierre Bruhns (5334050)
Medicine
Microbiology
Neuroscience
Biotechnology
Chemical Sciences not elsewhere classified
Immunology
Cancer
Infectious Diseases
Virology
Computational Biology
monomers &# 8212
monoclonal level contributes
fold lower compared
cambodian pediatric cohort
based microfluidic imaging
developing antibody response
broadly reactive antigen
dengue severity may
secreting cells relative
profiled single igg
300 single igg
secreting cells
specific antibody
reactive antibodies
hospitalized dengue
virion surface
using droplet
threatening illness
sufficient numbers
similar frequencies
serotype cross
secreted antibodies
qualitative differences
outcomes ranging
inefficient generation
forms homodimers
findings suggest
detected exclusively
acute denv1
<p><b>A.</b> (Left) Schematic representation of the DropMap bioassay. Created in Biorender. (Right) Representative images of the DropMap bioassay including relocation onto the beadline in droplets of DENV1 sE-dimer (FITC), DENV1 sE (TRITC) and DENV2 sE-dimer (IR) in the absence (0 nM; negative control) or presence of anti-DENV1/DENV2 E protein mAb ADI24-294 (10nM; positive control). <b>B.</b> IgG secreting rate from (left) total IgG-SC or from (right) IgG-SC binding to sE and/or sE-dimer of DENV1 or DENV2 in PBMCs from hospitalized dengue patients and subclinical cases at day 5 post RT-qPCR confirmed DENV infection, corresponding to day 7-8 after onset of symptoms. Horizontal bars represent median and IQR. Each dot represents one IgG-SC. <i>p</i> values were calculated using two-by-two contrast comparisons after linear modelling. ns, not significant<b>. C.</b> Proportion of IgG-SC not binding any DENV E protein (non-specific IgG-SC; white boxes), or binding at least one DENV1 E protein (to sE and/or sE-dimer; green boxes), or binding at least one DENV2 E protein (to sE and/or sE-dimer; blue boxes) in age-matched Cambodian healthy donors (HD) or in individuals infected with either (left) DENV1 or (right) DENV2. Patients with subclinical dengue (S) or hospitalized dengue (H) were numbered and stratified by infecting DENV serotype.</p>
2026-10-01T17:54:57Z
Image
Figure
10.1371/journal.ppat.1014552.g002
https://figshare.com/articles/figure/_p_Significantly_lower_IgG-SC_numbers_in_hospitalized_patients_compared_to_subclinical_cases_p_/34050117
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