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<p>Characterization of the anti-E protein antibody response in plasma of subclinical and hospitalized post-primary DENV infected patients.</p> Amandine Trouchet (25157966) Sokchea Lay (9857198) Matteo Broketa (19690885) Pablo Canales-Herrerias (25157969) Angga Perima (19690879) Bruno Iannascoli (17818357) Sotheary Sann (12039614) Borita Heng (25157972) Sowath Ly (160224) Veasna Duong (106893) Gaël A. Millot (25157975) Laura Walker (4841532) Patrick England (28458) Giovanna Barba-Spaeth (12066108) Tineke Cantaert (68002) Pierre Bruhns (5334050) Medicine Microbiology Neuroscience Biotechnology Chemical Sciences not elsewhere classified Immunology Cancer Infectious Diseases Virology Computational Biology monomers &# 8212 monoclonal level contributes fold lower compared cambodian pediatric cohort based microfluidic imaging developing antibody response broadly reactive antigen dengue severity may secreting cells relative profiled single igg 300 single igg secreting cells specific antibody reactive antibodies hospitalized dengue virion surface using droplet threatening illness sufficient numbers similar frequencies serotype cross secreted antibodies qualitative differences outcomes ranging inefficient generation forms homodimers findings suggest detected exclusively acute denv1 <p><b>A.</b> Inclusion scheme of individuals with subclinical (blue, n = 6) and hospitalized (red, n = 9) post-primary DENV1 or DENV2 infections. Created in Biorender. <b>B.</b> Mean Fluorescence Intensity (MFI) of IgG binding to EDIII derived from DENV1-4 and ZIKV of plasma samples of subclinical (S: n = 6; numbered S1 to S6) and hospitalized (H; n = 9; numbered H1 to H9) individuals infected with DENV1 (left) or DENV2 (right) at day of inclusion <b>C.</b> Heatmap representation of MFI of IgG binding to EDIII derived from DENV1-4 and ZIKV. Each row represents one individual. Hospitalized patients are subcategorized as individuals with uncomplicated dengue (D), n = 4 or dengue with warning signs (DWWS), n = 5. <b>D.</b> Mean Fluorescence Intensity of IgG binding to DENV1 sE-dimer (green) or sE (orange), or DENV2 sE-dimer (blue) or sE (brown) in day 5 plasma samples of subclinical (n = 4) and hospitalized (n = 9) individuals infected with DENV1 (left) or DENV2 (right). IgG binding was measured by using a multiplex microsphere immunoassay (MIA) and are represented as median fluorescence intensity (MFI). <b>E.</b> Neutralizing antibody titers against DENV1 and DENV2 were compared between subclinical cases (blue; n = 5) and hospitalized patients (red; n = 9) on D5 plasma and the data shown as focus reduction neutralization test 50 (FRNT50). Each point represents an individual; for 2 subclinical cases, not sufficient plasma was retrieved to complete all the assays. Median and 95% confidence interval are shown.</p> 2026-10-01T17:54:57Z Image Figure 10.1371/journal.ppat.1014552.g001 https://figshare.com/articles/figure/_p_Characterization_of_the_anti-E_protein_antibody_response_in_plasma_of_subclinical_and_hospitalized_post-primary_DENV_infected_patients_p_/34050114 CC BY 4.0