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oai:figshare.com:article/34050087 2026-10-01T18:00:19Z category_7 category_8 category_15 category_21 category_873 category_46 category_64 category_132 category_134 category_135 portal_5 item_type_1 month_year_10_2026

Affinity reference curves used for KD calculations from DropMap data. Amandine Trouchet (25157966) Sokchea Lay (9857198) Matteo Broketa (19690885) Pablo Canales-Herrerias (25157969) Angga Perima (19690879) Bruno Iannascoli (17818357) Sotheary Sann (12039614) Borita Heng (25157972) Sowath Ly (160224) Veasna Duong (106893) Gaël A. Millot (25157975) Laura Walker (4841532) Patrick England (28458) Giovanna Barba-Spaeth (12066108) Tineke Cantaert (68002) Pierre Bruhns (5334050) Medicine Microbiology Neuroscience Biotechnology Chemical Sciences not elsewhere classified Immunology Cancer Infectious Diseases Virology Computational Biology monomers &# 8212 monoclonal level contributes fold lower compared cambodian pediatric cohort based microfluidic imaging developing antibody response broadly reactive antigen dengue severity may secreting cells relative profiled single igg 300 single igg secreting cells specific antibody reactive antibodies hospitalized dengue virion surface using droplet threatening illness sufficient numbers similar frequencies serotype cross secreted antibodies qualitative differences outcomes ranging inefficient generation forms homodimers findings suggest detected exclusively acute denv1 Representation of DropMap slope vs KD data obtained using Biolayer Interferometry (Octet) of a series of anti-E protein monoclonal antibodies binding: (A) DENV1 sE and DENV1 sE-dimer, (B) DENV1 sE, DENV1 sE-dimer and DENV2 sE-dimer, (C) DENV2 sE and DENV2 sE-dimer, (D) DENV2 sE, DENV2 sE-dimer and DENV1 sE-dimer. (A-D) Power regressions and their formula are represented for each antigen relocation within a condition (i.e., if the antibody binds two or three different antigens, and from which dengue serotype). Each dot represents the mean of the measures for one anti-sE-protein mAb performed in triplicate; SEM are represented. Note: The competition between cross-reactive mAbs binding 2 or 3 different E antigens was addressed by calibrating the assay under the same multiplex conditions used for patient-derived IgG-secreting cells (Figs 2–4 and S4–S8). Here, we generated separate KD–DropMap slope relationships for the relevant binding configurations, including droplets in which two or three antigens were relocated. These curves were established using monoclonal anti-DENV antibodies with independently determined KD values, measured with the same antigen combinations and assay geometry. (TIFF) 2026-10-01T17:54:57Z Image Figure 10.1371/journal.ppat.1014552.s003 https://figshare.com/articles/figure/_p_Affinity_reference_curves_used_for_K_sub_D_sub_calculations_from_DropMap_data_p_/34050087 CC BY 4.0