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oai:figshare.com:article/34049542 2026-10-01T17:47:07Z category_4 category_12 category_13 category_14 category_15 category_16 category_64 category_128 portal_5 item_type_1 month_year_10_2026

<p><i>unc-103</i>(G427R) is a gain-of-function allele that inhibits egg laying.</p> Xinyu Huang (679541) Sudharsan Kannan (25158064) Gail A. Robertson (15007385) Han Wang (254423) Biochemistry Cell Biology Genetics Molecular Biology Neuroscience Physiology Cancer Hematology regulate neuronal excitability promoting neuron ala influence channel gating hallmark cyclic nucleotide genetic analysis demonstrated forward genetics reveals electrophysiological analysis showed dependent channel activation calcium imaging showed binding homology domains adjacent channel subunits accelerates activation kinetics subfamily kcnh channels conserved molecular mechanism important sleep regulator findings identify egl div >< p caenorhabditis elegans </ similarly suppresses sleep 2 functions cell elegans </ suppresses sleep kcnh voltage vivo </ central sleep conserved cnbhd shifts voltage second substitution negative potentials intrinsic ligand g574r ), function effects corresponding residue <p>(<b>A</b>) Amino acid sequence alignment of the CNBHDs from mEAG1, EGL-2, hERG, and UNC-103. Secondary structure elements from the mEAG1 CNBHD (PDB: 4F8A) are indicated above the alignment, with three α-helices (αA–αC) and nine β-strands (β1–β9) indicated by coils and arrows, respectively. The red star indicates the conserved glycine residue corresponding to mEAG1(G588), EGL-2(G574), UNC-103(G427), and hERG1a(G749). (<b>B-C</b>) Representative brightfield images and quantification of the eggs retained in the uterus of adult animals with the indicated genotypes. <i>gof</i>, gain-of-function; <i>lof</i>, loss-of-function. White asterisks mark the vulva position. (B) A, anterior; D, dorsal. Scale bar, 50 μm. (C) Numbers shown on the bars indicate the total number of animals scored for each genotype. Mean ± SEM are shown. ****, p < 0.0001. One-way ANOVA with Dunnett’s multiple-comparisons test for comparing each mutant genotype with wild type.</p> 2026-10-01T17:46:48Z Image Figure 10.1371/journal.pgen.1012255.g007 https://figshare.com/articles/figure/_p_i_unc-103_i_G427R_is_a_gain-of-function_allele_that_inhibits_egg_laying_p_/34049542 CC BY 4.0