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oai:figshare.com:article/34049503 2026-10-01T17:46:53Z category_4 category_12 category_13 category_14 category_15 category_16 category_64 category_128 portal_5 item_type_1 month_year_10_2026

<p><i>egl-2(</i><i>G574E)</i> and <i>egl-2(G574R</i>) mutants are defective in egg laying.</p> Xinyu Huang (679541) Sudharsan Kannan (25158064) Gail A. Robertson (15007385) Han Wang (254423) Biochemistry Cell Biology Genetics Molecular Biology Neuroscience Physiology Cancer Hematology regulate neuronal excitability promoting neuron ala influence channel gating hallmark cyclic nucleotide genetic analysis demonstrated forward genetics reveals electrophysiological analysis showed dependent channel activation calcium imaging showed binding homology domains adjacent channel subunits accelerates activation kinetics subfamily kcnh channels conserved molecular mechanism important sleep regulator findings identify egl div >< p caenorhabditis elegans </ similarly suppresses sleep 2 functions cell elegans </ suppresses sleep kcnh voltage vivo </ central sleep conserved cnbhd shifts voltage second substitution negative potentials intrinsic ligand g574r ), function effects corresponding residue <p>Quantification of the eggs retained in the uterus of adult animals with the indicated genotypes. Numbers shown on the bars indicate the total number of animals scored for each genotype. The results are consistent with the idea that <i>egl-2(G574R)</i> is a stronger gain-of-function allele than <i>egl-2 (G574E)</i>. Mean ± SEM are shown. ****, p < 0.0001. One-way ANOVA with Dunnett’s multiple-comparisons test.</p> <p>(TIF)</p> 2026-10-01T17:46:48Z Image Figure 10.1371/journal.pgen.1012255.s003 https://figshare.com/articles/figure/_p_i_egl-2_i_i_G574E_i_and_i_egl-2_G574R_i_mutants_are_defective_in_egg_laying_p_/34049503 CC BY 4.0