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<p>Conservation of the core architecture of EAG potassium channels.</p>
Xinyu Huang (679541)
Sudharsan Kannan (25158064)
Gail A. Robertson (15007385)
Han Wang (254423)
Biochemistry
Cell Biology
Genetics
Molecular Biology
Neuroscience
Physiology
Cancer
Hematology
regulate neuronal excitability
promoting neuron ala
influence channel gating
hallmark cyclic nucleotide
genetic analysis demonstrated
forward genetics reveals
electrophysiological analysis showed
dependent channel activation
calcium imaging showed
binding homology domains
adjacent channel subunits
accelerates activation kinetics
subfamily kcnh channels
conserved molecular mechanism
important sleep regulator
findings identify egl
div >< p
caenorhabditis elegans </
similarly suppresses sleep
2 functions cell
elegans </
suppresses sleep
kcnh voltage
vivo </
central sleep
conserved cnbhd
shifts voltage
second substitution
negative potentials
intrinsic ligand
g574r ),
function effects
corresponding residue
<p>Alignment of the protein sequences spanning the region from the PAS domain to the cyclic nucleotide-binding homology domain (CNBHD) of EAG channels from the following species: <i>Caenorhabditis elegans (EGL-2,</i> NP_503402.3)<i>, Mus musculus (mEag1,</i> NP_034730.1), <i>Rattus norvegicus (rEag1,</i> NP_113930.1), <i>Homo sapiens (hEAG1,</i> NP_758872.1), <i>Xenopus laevis (fEAG1,</i> XP_041418453.1), and <i>Drosophila melanogaster (dEAG,</i> NP_001036275.1). Conserved residues are highlighted in black. Functional domains are indicated above the alignment, including the PAS domain, the voltage sensor domain (S1–S4), the pore-forming segments (S5–S6), the potassium ion selectivity filter (SF), the CNBHD, and the intrinsic ligand motif. Residue numbering at the top corresponds to EGL-2 isoform a. The red star indicates the highly conserved glycine residue that is mutated in <i>egl-2(sy985)</i> and <i>egl-2(tan200)</i>. C-terminal sequences to the intrinsic ligand are omitted.</p> <p>(TIF)</p>
2026-10-01T17:46:48Z
Image
Figure
10.1371/journal.pgen.1012255.s001
https://figshare.com/articles/figure/_p_Conservation_of_the_core_architecture_of_EAG_potassium_channels_p_/34049485
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