2026-10-11T05:28:39Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34049279 2026-10-01T17:46:28Z category_4 category_8 category_12 category_15 category_16 category_39 category_46 category_734 category_132 category_134 portal_5 item_type_1 month_year_10_2026

<p>Increased C1q deposition in glomeruli of MRL/lpr mice treated with curli.</p> Kaitlyn Grando (12527650) Molly Elkins (9980979) Lauren Nicastro (25157996) Shingo Bessho (13257073) Sophia Olubajo (24393496) Anita Kowal (25157999) Vincent Tam (2583448) Roberto Caricchio (18920) David Pisetsky (5618543) Gregg J. Silverman (7949903) Çagla Tükel (45788) Biochemistry Microbiology Cell Biology Neuroscience Physiology Ecology Immunology Biological Sciences not elsewhere classified Infectious Diseases Virology specific elisa confirmed significantly affect proteinuria chromatin autoantibody responses div >< p systemic lupus erythematosus demonstrated increased anti f1 mice compared biofilms drive anti f1 mice previously demonstrated autoantibody levels prone mice older mice lpr mice young nzbxw treated controls study uncovers spontaneous disease salmonella </ prone nzbxw prominent feature often marked novel mechanism increasing morbidity either model disease stage disease flares coli </ autoimmune manifestations amyloidogenic protein already high <p>MRL/lpr mice were intraperitoneally injected weekly with (<b>A</b>) PBS or (<b>B</b>) curli purified from <i>Salmonella</i> Typhimurium for 6 weeks and euthanized at 13 weeks old. Frozen tissue sections were stained with goat anti-mouse IgG-AlexaFluor405 (blue), rat anti-mouse C3-AlexaFluor647 (red), and rat anti-mouse C1q-FITC (green). (<b>A-B</b>) Representative images of glomeruli by confocal microscopy (63X objective). Sections shown are from two individual mice per group, scale bars represent 20 µm. (<b>C-F</b>) Images were quantified in ImageJ for mean fluorescence intensity (MFI) inside glomeruli of (<b>C</b>) IgG, (<b>D</b>) C3, (<b>E</b>) C1q, and (<b>F</b>) area was measured, within at least 15 glomeruli per mouse and averaged. Statistics: (<b>C-F</b>) Unpaired t-test (**p < 0.01).</p> 2026-10-01T17:56:08Z Image Figure 10.1371/journal.ppat.1014666.g006 https://figshare.com/articles/figure/_p_Increased_C1q_deposition_in_glomeruli_of_MRL_lpr_mice_treated_with_curli_p_/34049279 CC BY 4.0