2026-10-12T20:23:30Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34049273 2026-10-01T17:46:25Z category_4 category_8 category_12 category_15 category_16 category_39 category_46 category_734 category_132 category_134 portal_5 item_type_1 month_year_10_2026

<p>Anti-C1q autoantibody levels are exacerbated in curli-treated MRL/lpr mice.</p> Kaitlyn Grando (12527650) Molly Elkins (9980979) Lauren Nicastro (25157996) Shingo Bessho (13257073) Sophia Olubajo (24393496) Anita Kowal (25157999) Vincent Tam (2583448) Roberto Caricchio (18920) David Pisetsky (5618543) Gregg J. Silverman (7949903) Çagla Tükel (45788) Biochemistry Microbiology Cell Biology Neuroscience Physiology Ecology Immunology Biological Sciences not elsewhere classified Infectious Diseases Virology specific elisa confirmed significantly affect proteinuria chromatin autoantibody responses div >< p systemic lupus erythematosus demonstrated increased anti f1 mice compared biofilms drive anti f1 mice previously demonstrated autoantibody levels prone mice older mice lpr mice young nzbxw treated controls study uncovers spontaneous disease salmonella </ prone nzbxw prominent feature often marked novel mechanism increasing morbidity either model disease stage disease flares coli </ autoimmune manifestations amyloidogenic protein already high <p>(<b>A</b>) Experimental scheme: MRL/lpr mice at age 6 weeks underwent weekly intraperitoneal injections of curli, purified from <i>Salmonella</i> Typhimurium biofilms or PBS for untreated controls, and periodic serum samples, taken 24 hours after each injection, for 6 weeks. (<b>B-C</b>) ELISA assays detected anti-dsDNA antibodies in serum diluted (<b>B</b>) 1:250 or (<b>C</b>) 1:500. (<b>D</b>) ELISA assay detected anti-C1q antibodies in serum diluted 1:50, normalized to the O.D. of the positive control untreated MRL/lpr serum. Dotted lines indicate relative O.D. of MRL/lpr positive control serum (top line) or negative control with no serum added (bottom line). Week -1 denotes baseline samples collected from each group one week before the first injection (Week 0). Statistics: Two-way ANOVA with Sidak’s multiple comparisons, comparisons within legends indicating overall column factor, (*p < 0.05, **p < 0.01).</p> 2026-10-01T17:56:08Z Image Figure 10.1371/journal.ppat.1014666.g004 https://figshare.com/articles/figure/_p_Anti-C1q_autoantibody_levels_are_exacerbated_in_curli-treated_MRL_lpr_mice_p_/34049273 CC BY 4.0