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oai:figshare.com:article/34049264 2026-10-01T17:46:21Z category_4 category_8 category_12 category_15 category_16 category_39 category_46 category_734 category_132 category_134 portal_5 item_type_1 month_year_10_2026

<p>Anti-C1q autoantibodies are induced by curli administration or <i>Salmonella</i> Typhimurium infection.</p> Kaitlyn Grando (12527650) Molly Elkins (9980979) Lauren Nicastro (25157996) Shingo Bessho (13257073) Sophia Olubajo (24393496) Anita Kowal (25157999) Vincent Tam (2583448) Roberto Caricchio (18920) David Pisetsky (5618543) Gregg J. Silverman (7949903) Çagla Tükel (45788) Biochemistry Microbiology Cell Biology Neuroscience Physiology Ecology Immunology Biological Sciences not elsewhere classified Infectious Diseases Virology specific elisa confirmed significantly affect proteinuria chromatin autoantibody responses div >< p systemic lupus erythematosus demonstrated increased anti f1 mice compared biofilms drive anti f1 mice previously demonstrated autoantibody levels prone mice older mice lpr mice young nzbxw treated controls study uncovers spontaneous disease salmonella </ prone nzbxw prominent feature often marked novel mechanism increasing morbidity either model disease stage disease flares coli </ autoimmune manifestations amyloidogenic protein already high <p>(<b>A-B</b>) Mouse serum was assayed using a multiplex Luminex platform and antibodies quantified by mean fluorescence intensity (MFI); only anti-C1q antibodies are shown here, see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1014666#ppat.1014666.s001" target="_blank">S1 Fig</a> for other antigens. 6-8 week old (<b>A</b>) C57BL/6 mice and (<b>B</b>) NZBxW/F1 mice were injected intraperitoneally with curli purified from <i>Salmonella</i> Typhimurium (STm) biofilms, or sterile PBS for controls, weekly for (<b>A</b>) 12 weeks or (<b>B</b>) 5 weeks. (<b>C-F</b>) Anti-C1q antibodies in serum (diluted 1:50) were detected using ELISA, normalized to aliquots of serum from an untreated aged MRL/lpr mouse as a positive control (1:50, top dotted line) and a negative control without serum (bottom dotted line). (<b>C</b>) C57BL/6 mice were injected intraperitoneally with 100 µg curli purified from STm biofilms, 100 µg/kg LPS, 50 µg curli and 50 µg/kg LPS, or PBS for untreated controls, weekly for 3 weeks<b>.</b> (<b>D</b>) C57BL/6 mice were injected intraperitoneally with 100 µg curli purified from the biofilm of an <i>E. coli</i> isolate from the human microbiome (hEC), an <i>E. coli</i> isolate from the mouse microbiome (mEC), STm biofilms, or PBS for untreated controls, weekly for 4 weeks<b>.</b> (<b>E</b>) 129Sv mice were infected intragastrically with 10⁷ CFU of wild-type (WT) STm strain IR715, the isogenic curli-deficient mutant <i>csgBA</i>, or LB broth for uninfected controls, in two replicate experiments, and Week 6 serum was assayed. (<b>F</b>) C57BL/6 mice were infected intragastrically with 10⁷ CFU of STm <i>spiB</i> or <i>invAspiB</i> mutant, or LB broth for uninfected controls, and monitored for 10 weeks. Week -1 denotes baseline samples collected from each group one week before the first injection (Week 0). Statistics: (<b>A-B</b>) Unpaired t-test, (<b>C-E</b>) One-way ANOVA, or (<b>E</b>) Mixed-effects analysis, with Tukey’s multiple comparisons, comparisons within the legend indicating overall column factor (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).</p> 2026-10-01T17:56:08Z Image Figure 10.1371/journal.ppat.1014666.g001 https://figshare.com/articles/figure/_p_Anti-C1q_autoantibodies_are_induced_by_curli_administration_or_i_Salmonella_i_Typhimurium_infection_p_/34049264 CC BY 4.0