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<p><i>MARCHF5</i> KO induces apoptosis.</p>
Prasanth Viswanathan (10314782)
Justine R. Bersonda (25157885)
Jackson Gill (25157888)
Alyssandra Navarro (25157891)
Allison C. Farrar (25157894)
Thomas Caldarera (25157897)
Andrew Murphy (341397)
Daniel Dunham (25157900)
Karl W. Boehme (11384016)
Mark Manzano (25157903)
Biochemistry
Cell Biology
Genetics
Molecular Biology
Cancer
Hematology
Infectious Diseases
Virology
systems biology approach
regulating mitochondrial homeostasis
primary effusion lymphoma
immunoprecipitation experiments show
endogenous reciprocal co
emerging clinical relevance
cell viability due
newly appreciated interaction
causes several malignancies
unexpected genetic interaction
findings thus provide
div >< p
marchf5 oncogenes may
pel cells suggesting
mcl1 motivated us
genetic interaction
tumor cells
causes resistance
thus investigated
therapeutic potential
survival role
significant increase
protein complex
previously shown
pel ).
mechanistic link
kaposi ’
fitness advantage
e3 ligase
drug development
dimerization activities
crispr knockout
combination therapies
chemotherapy regimens
attractive target
associated herpesvirus
apoptotic signal
apoptotic activities
antiviral signaling
<p>Apoptosis was measured using A. Caspase 3/7 activity using the Caspase-Glo 3/7 Luminescence Assay or B. levels of Annexin V-7/AAD using the FITC Annexin V Apoptosis Detection Kit with 7-AAD for flow cytometry analysis from BC-3 and BCBL-1 iCas9 <i>MARCHF5</i> KO cells harvested at Day 6 of the growth curves from <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1014665#ppat.1014665.g002" target="_blank">Fig 2</a>. Statistical differences were calculated using one-way ANOVA with Tukey’s multiple comparison post-hoc test (n = 3-5). Adjusted <i>p</i> values reflect post-hoc comparisons between g<i>AAVS1</i> to either <i>MARCHF5</i> gRNA. Error bars, standard error of the mean.</p>
2026-10-01T17:54:28Z
Image
Figure
10.1371/journal.ppat.1014665.g003
https://figshare.com/articles/figure/_p_i_MARCHF5_i_KO_induces_apoptosis_p_/34049123
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