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<p>Binding and functional activity of V1WT_06, V1WT_41 and VA14_26.</p>
Ayush Upadhyay (25157573)
Jeffrey Seow (9415776)
Yilmaz Alguel (24840073)
Joseph Newman (5638340)
Nazia Thakur (9904162)
Abigail L. Hay (25157576)
Jerry C. H. Tam (13955938)
Andrea Nans (5176226)
Richard J. Orton (7832015)
Dalan Bailey (193037)
Peter Cherepanov (369)
Katie J. Doores (346210)
Biophysics
Microbiology
Evolutionary Biology
Ecology
Immunology
Biological Sciences not elsewhere classified
Hematology
Infectious Diseases
Virology
v1wt_06 contact residues
therapeutic antibody development
reactive monoclonal antibodies
mab v1wt_06 binds
increasing spillover potential
human population presents
heavy chain alone
future therapeutic use
confer broad sarbecovirus
circulating viruses suggesting
2 variant neutralisation
2 variants
xlink ">
virus entry
urgent need
rationale design
rational design
neutralising epitopes
including xec
ideal candidates
highly conserved
functionally constrained
discover cross
differing angles
continuing evolution
ace2 competition
<p><b>A)</b> Binding of IgG and Fab to recombinant RBDs of Sarbecoviruses from different clades by ELISA including SARS-CoV-2 (clade 1b), WIV-1 (clade 1a), Rs4321 (clade 1a), RacCS203 (clade 2), Rc-o319 (clade 5), and RhGB07 (clade 3). Experiments were run in duplicate and repeated twice. A representative assay is shown. Fab binding is shown with the dotted line and IgG binding with the full line. <b>B)</b> Neutralising activity of IgGs against SARS-CoV-2 and variants of concern as well as SARS-CoV-1 and WIV-1. <b>C)</b> IC<sub>50</sub> values for neutralisation against SARS-CoV-2 variants of concern and other sarbecoviruses that utilise human-ACE2. For A–C, the mean value is plotted with error bars showing ± standard deviation (s.d.). <b>D)</b> Ability of IgGs to inhibit the interaction between cell surface human ACE2 and soluble SARS-CoV-2 and WIV-1 RBDs. IgGs were pre-incubated with fluorescently labelled RBD before addition to HeLa-ACE2 cells. The percentage reduction in mean fluorescence intensity is reported. Experiments were performed in duplicate. <b>E)</b> Superimposed 3D structures of the V1WT_06 (dark blue), V1WT_41 (purple), and VA14_26 (red) around WIV-1 RBD (light blue, from PDB 8WLZ). The data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003882#pbio.3003882.s010" target="_blank">S1 Data</a>.</p>
2026-10-01T17:40:02Z
Image
Figure
10.1371/journal.pbio.3003882.g001
https://figshare.com/articles/figure/_p_Binding_and_functional_activity_of_V1WT_06_V1WT_41_and_VA14_26_p_/34048933
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