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oai:figshare.com:article/34048930 2026-10-01T17:40:20Z category_1 category_8 category_24 category_39 category_46 category_734 category_128 category_132 category_134 portal_5 item_type_1 month_year_10_2026

<p>Raw image of western blot used in S1C Fig.</p> Ayush Upadhyay (25157573) Jeffrey Seow (9415776) Yilmaz Alguel (24840073) Joseph Newman (5638340) Nazia Thakur (9904162) Abigail L. Hay (25157576) Jerry C. H. Tam (13955938) Andrea Nans (5176226) Richard J. Orton (7832015) Dalan Bailey (193037) Peter Cherepanov (369) Katie J. Doores (346210) Biophysics Microbiology Evolutionary Biology Ecology Immunology Biological Sciences not elsewhere classified Hematology Infectious Diseases Virology v1wt_06 contact residues therapeutic antibody development reactive monoclonal antibodies mab v1wt_06 binds increasing spillover potential human population presents heavy chain alone future therapeutic use confer broad sarbecovirus circulating viruses suggesting 2 variant neutralisation 2 variants xlink "> virus entry urgent need rationale design rational design neutralising epitopes including xec ideal candidates highly conserved functionally constrained discover cross differing angles continuing evolution ace2 competition <p>Lysates of cells transfected with bat Sarbecovirus Spikes were boiled in nonreducing loading buffer for 5 min. SDS-PAGE and membrane-transfer was conducted as per standard methods. The membrane was blocked and incubated with primary mouse anti-FLAG tag antibody. For tagged Sarbecovirus Spikes, Spike expression was detected using secondary anti-mouse antibody, at expected band size of ~180 kDa. The blot was captured using LiCor Odyssey Fc using 700 nm (ladder) and 800 nm (sample) channel. The membrane was subsequently incubated with anti-GAPDH antibody and detected using a similar method at expected size of 36 kDa.</p> <p>(TIF)</p> 2026-10-01T17:40:02Z Image Figure 10.1371/journal.pbio.3003882.s011 https://figshare.com/articles/figure/_p_Raw_image_of_western_blot_used_in_S1C_Fig_p_/34048930 CC BY 4.0