2026-10-06T23:46:16Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34048219
2026-10-01T17:31:11Z
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item_type_3
month_year_10_2026
<p>Hsp40 qRT-PCR data.</p>
Mohamed Lotfy (18599184)
Fatema Al Sawai (25157749)
Dhoha Alyammahi (25157752)
Biduth Kundu (18599205)
Biochemistry
Genetics
Molecular Biology
Evolutionary Biology
Developmental Biology
Marine Biology
xlink "> flies
increased trehalose levels
excess dietary sugar
functional locomotor impairment
drosophila melanogaster </
induced metabolic dysfunction
antioxidant enzyme activities
impaired locomotor performance
gene expression levels
antioxidant enzyme activity
15 mm ).
xlink "> high
without metformin treatment
related gene expression
metformin modulates survival
drosophila </
locomotor performance
locomotor activity
gene expression
t2dm ).
15 %),
related genes
metabolic parameters
metabolic disorders
treatment groups
treated groups
tractable model
studying diet
study investigates
quantified relative
molecular effects
modulatory effects
major contributor
including improvements
gst ),
findings support
diabetes model
changes within
carbohydrate imbalance
alterations relative
also associated
5 mm
10 days
<div>
<p>Background</p><p>Excess dietary sugar is a major contributor to metabolic disorders, including type 2 diabetes mellitus (T2DM). This study investigates the physiological, biochemical, behavioral, and molecular effects of high-sucrose diets in <i>Drosophila melanogaster</i> and evaluates the modulatory effects of metformin under these conditions.</p>
<p>Methods</p><p>Flies were reared on normal and high-sucrose diets (5% and 15%), with or without metformin treatment (5 mM and 15 mM). Pupation, survival, locomotor activity (negative geotaxis), trehalose levels, antioxidant enzyme activities (SOD, GST), and expression of stress-related genes (Mn-SOD, Cu/Zn-SOD, MTH, CAT, Hsp40, Hsp70) were assessed after 10 days of treatment. Gene expression levels were quantified relative to the normal control group using RpS20 as the housekeeping reference.</p>
<p>Results</p><p>High-sucrose diets significantly reduced pupation and survival rates, impaired locomotor performance, increased trehalose levels, and decreased antioxidant enzyme activities. These diets were also associated with downregulation of stress- and antioxidant-related genes. Metformin treatment partially attenuated several of these alterations relative to the corresponding sucrose-treated groups, including improvements in pupation, survival, locomotor performance, metabolic parameters, antioxidant enzyme activity, and gene expression.</p>
<p>Conclusion</p><p>High-sucrose diets induced metabolic disturbances in <i>Drosophila melanogaster</i>, including impaired developmental progression and survival, functional locomotor impairment, carbohydrate imbalance, oxidative stress, and suppression of stress-related gene expression. Metformin partially attenuated several of these changes within the corresponding sucrose-treatment groups. These findings support the utility of <i>Drosophila melanogaster</i> as a tractable model for studying diet-induced metabolic dysfunction and evaluating pharmacological interventions.</p></div>
2026-10-01T17:31:03Z
Dataset
Dataset
10.1371/journal.pone.0359638.s006
https://figshare.com/articles/dataset/_p_Hsp40_qRT-PCR_data_p_/34048219
CC BY 4.0