2026-10-10T12:19:06Z
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2026-10-01T17:30:34Z
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<p>Cytokine gene expression changes in pSIOs following LPS treatment.</p>
Sumin Park (1418677)
Boram Lee (549836)
Dahye Kim (4750968)
Sheet Sunirmal (25157743)
Min Gook Lee (25157746)
Haesun Lee (13907847)
Sun A. Ock (17671438)
Jae Gyu Yoo (18291035)
Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Physiology
Chemical Sciences not elsewhere classified
Science Policy
Immunology
Biological Sciences not elsewhere classified
Infectious Diseases
potentially reducing reliance
cell models fail
adult jejunum stimulated
functional categories associated
bile acid signaling
div >< p
cytokine gene expression
studying intestinal inflammation
intestinal tract
associated diseases
receptor signaling
inflammatory signaling
vitro </
valuable platform
transcriptome analysis
time pcr
thereby posing
related pathways
proteins involved
physiologically relevant
nutrient transport
major threat
inflammatory stress
induced alterations
increased tlr4
including enrichment
immunofluorescence staining
findings demonstrate
enriched pathways
barrier maintenance
barrier integrity
animal testing
analyzing changes
altered expression
3rs principle
<p>(A) Immunofluorescence staining of TLR4 in pSIOs after LPS (20 μM) treatment. Nuclei were stained with DAPI (blue), and TLR4 expression is shown in green. Representative images suggest higher TLR4 immunofluorescence in the LPS-treated group than in the control group. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each treatment condition in each biological replicate. Scale bar = 100 μm. (B) qRT-PCR analysis of pro- and anti-inflammatory cytokines in pSIOs after LPS (20 μM) exposure. Pro-inflammatory cytokines <i>TNF-α</i>, <i>IL-1β</i>, and <i>IL-8</i> were significantly upregulated in the LPS-treated group compared with the control group. Anti-inflammatory cytokines <i>IL-4</i> and <i>IL-10</i> also showed significantly increased expression, whereas <i>TGF-β</i> expression was increased but did not reach statistical significance following LPS stimulation. Data are presented as the mean ± SD from three biological replicates (n = 3); each biological replicate was analyzed using five technical replicates. *<i>p</i> < 0.05, **<i>p</i> < 0.01.</p>
2026-10-01T17:30:26Z
Image
Figure
10.1371/journal.pone.0359086.g004
https://figshare.com/articles/figure/_p_Cytokine_gene_expression_changes_in_pSIOs_following_LPS_treatment_p_/34048153
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