2026-10-09T22:43:08Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34048147
2026-10-01T17:30:31Z
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<p>Characterization of porcine small intestinal organoids (pSIOs).</p>
Sumin Park (1418677)
Boram Lee (549836)
Dahye Kim (4750968)
Sheet Sunirmal (25157743)
Min Gook Lee (25157746)
Haesun Lee (13907847)
Sun A. Ock (17671438)
Jae Gyu Yoo (18291035)
Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Physiology
Chemical Sciences not elsewhere classified
Science Policy
Immunology
Biological Sciences not elsewhere classified
Infectious Diseases
potentially reducing reliance
cell models fail
adult jejunum stimulated
functional categories associated
bile acid signaling
div >< p
cytokine gene expression
studying intestinal inflammation
intestinal tract
associated diseases
receptor signaling
inflammatory signaling
vitro </
valuable platform
transcriptome analysis
time pcr
thereby posing
related pathways
proteins involved
physiologically relevant
nutrient transport
major threat
inflammatory stress
induced alterations
increased tlr4
including enrichment
immunofluorescence staining
findings demonstrate
enriched pathways
barrier maintenance
barrier integrity
animal testing
analyzing changes
altered expression
3rs principle
<p>Relative mRNA expression in porcine ear fibroblasts (pEFs) and pSIOs was analyzed using qRT-PCR. Intestinal epithelial markers (<i>VIL1</i>, <i>LYZ</i>, and <i>CHGA</i>) (A–C), intestinal stem cell markers (<i>LGR5</i>, <i>OLFM4</i>, and <i>SOX9</i>), and proliferation marker <i>MKI67</i> (D–G) were significantly upregulated in pSIOs compared with pEFs. Data are presented as the mean ± SD from three biological replicates (n = 3); each biological replicate was analyzed using five technical replicates. ***<i>p</i> < 0.001.</p>
2026-10-01T17:30:26Z
Image
Figure
10.1371/journal.pone.0359086.g002
https://figshare.com/articles/figure/_p_Characterization_of_porcine_small_intestinal_organoids_pSIOs_p_/34048147
CC BY 4.0