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2026-10-01T17:29:06Z
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<p>Assessment of epithelial barrier integrity using TEER for different concentrations of barrier disruptors (ethanol – left, EGTA – right) and for different fluorescent contrast agents.</p>
Elena Monfort Sanchez (25157711)
Aaron F. Watson (25157714)
Tafhima Haider (14520521)
Despoina Chrysostomou (15147873)
Maelle Storder (25157717)
Nilanjan Mandal (25157720)
Paul Kelly (95510)
Julian R. Marchesi (165208)
James Avery (6193926)
Claire D. Bourke (12865193)
Alex J. Thompson (630738)
Biophysics
Biochemistry
Medicine
Cell Biology
Biotechnology
Environmental Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer
Infectious Diseases
type ii diabetes
numerous physiological processes
inflammatory bowel disease
g ., lps
g ., lactulose
epithelial barriers serve
determine barrier damage
assess barrier integrity
epithelial barrier integrity
epithelial barrier function
translational gastroenterology research
molecular weights comparable
maintaining tissue homeostasis
fluorescent contrast agents
different disease contexts
microfluidic ‘ gut
mimic substances used
div >< p
cellular monolayer models
study gut permeability
epithelial barrier
substances used
molecular weight
different conditions
body tissue
barrier assessment
translational assessment
gut epithelium
fluorescent assessment
wider adoption
vivo </
vitro </
various cells
pamps )).
nutrient absorption
neuroimmune communication
multiple drawbacks
microbiota regulation
methylene blue
key component
indicating suitability
external environment
drive enteropathy
diverse range
cost alternatives
clinically approved
clinical settings
<p><b>(A-F)</b> Relative TEER changes (0-3 hours) for each fluorescent contrast agent with different levels of barrier disruption and different contrast agent concentrations. Control values reperesent TEER values for wells where no disruptor was applied. Two biological repeats (n = 2), one technical repeat (N = 1). Error bars represent standard deviations. Inset points shown alongside bars represent values from individual experiments.</p>
2026-10-01T17:28:59Z
Image
Figure
10.1371/journal.pone.0358746.g002
https://figshare.com/articles/figure/_p_Assessment_of_epithelial_barrier_integrity_using_TEER_for_different_concentrations_of_barrier_disruptors_ethanol_left_EGTA_right_and_for_different_fluorescent_contrast_agents_p_/34048007
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