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DDB1-VprBP complex assembles into a homodimer via the LisH domain. Dianhong Wang (25157696) Weijia Ding (554415) Jingwei Xu (337549) Ruofan Li (1568194) Tamino Cairoli (25157699) Qi Liu (33068) Yiying Zhu (8675928) Ye Xiang (522776) Biophysics Biochemistry Cell Biology Evolutionary Biology Infectious Diseases Plant Biology Virology various restriction factors ring e3 ligase larger tet2 fragment findings could facilitate diverse substrate recruitment vprbp could enable vprbp adopt either xlink "> hiv two &# 8220 recruits diverse substrates bound host factors like domains adopt diverse vpr host cul4 &# 8220 vprbp mediates vprbp dimerization receptor vprbp &# 8221 structures highlight structural basis results pinpoint molecular architecture may assist lish domain key protein guided development divergent stoichiometries directly interact different vpr conformational dynamics accommodating vpr 1 vpr 1 hijacks (A) Schematic illustration of the domain organization of VprBP and DDB1. VprBP comprises an ARM-like domain (yellow), a LisH domain (orange), a WD40 domain (blue), and a C-terminal Acidic domain (magenta). DDB1 consists of three β-propeller domains (BPA, pink; BPB, green; BPC, cyan) and a C-terminal domain (CTD, purple). (B) Dimeric structure of the “ARM-up” DDB1-VprBP shown as ribbons. Domains in one DDB1-VprBP protomer are color-coded according to the scheme in panel A, while the symmetry-related protomer is colored gray. (C) Dimeric structure of the “ARM-down” DDB1-VprBP shown as ribbons. The domains are color-coded as in panel B. (D) LisH domains mediate the VprBP dimerization. Left, ribbon diagrams highlighting the LisH domains (blue and orange). Disulfide bonds stabilizing the connection between loop (residues 1019-1037) and α4 are highlighted in yellow sticks. The dashed lines indicate the unresolved loop (residues 880-1000). Right, detailed view of the leucine-rich hydrophobic core at the LisH dimer interface. 2026-10-01T17:28:02Z Image Figure 10.1371/journal.ppat.1014610.g001 https://figshare.com/articles/figure/_p_DDB1-VprBP_complex_assembles_into_a_homodimer_via_the_LisH_domain_p_/34047926 CC BY 4.0