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<p>AlphaFold prediction and cellular validation of the interaction between TET2 and Vpr.</p> Dianhong Wang (25157696) Weijia Ding (554415) Jingwei Xu (337549) Ruofan Li (1568194) Tamino Cairoli (25157699) Qi Liu (33068) Yiying Zhu (8675928) Ye Xiang (522776) Biophysics Biochemistry Cell Biology Evolutionary Biology Infectious Diseases Plant Biology Virology various restriction factors ring e3 ligase larger tet2 fragment findings could facilitate diverse substrate recruitment vprbp could enable vprbp adopt either xlink "> hiv two &# 8220 recruits diverse substrates bound host factors like domains adopt diverse vpr host cul4 &# 8220 vprbp mediates vprbp dimerization receptor vprbp &# 8221 structures highlight structural basis results pinpoint molecular architecture may assist lish domain key protein guided development divergent stoichiometries directly interact different vpr conformational dynamics accommodating vpr 1 vpr 1 hijacks <p><b>(A)</b> AlphaFold prediction of the Vpr-TET2 interaction. Residues involved in the interactions are shown as sticks. <b>(B)</b> Western blot analysis of TET2 co-transfected with Vpr or Vpr mutants in HEK293T cells. β-actin was used as an internal loading control. Vpr mutant Q65R, which is deficient in VprBP binding, was used as a negative control. (<b>C</b>) Statistical quantification of biological replicates. Densitometric quantification derived from the three independent experimental repeats. Data are presented as mean. Error bars represent the standard deviation (SD). <i>n.s.</i>, not statistically significant (<i>P</i> > 0.05); *, <i>P</i> < 0.05. (<b>D</b>) Functional validation of the Vpr C-terminal mutant in TET2 degradation. Western blot analysis showing that the Vpr C-terminal R88A mutant maintains its capacity to degrade TET2. Western blots were performed in three independent biological replicates (<i>n</i> = 3) with one representative blot shown. (<b>E</b>) Statistical quantification of biological replicates. Densitometric quantification derived from the three independent experimental repeats. Data are presented as mean. Error bars represent the standard deviation (SD). <i>n.s.</i>, not statistically significant (<i>P</i> > 0.05); *, <i>P</i> < 0.05; ***, <i>P</i> < 0.001.</p> <p>(TIF)</p> 2026-10-01T17:28:02Z Image Figure 10.1371/journal.ppat.1014610.s018 https://figshare.com/articles/figure/_p_AlphaFold_prediction_and_cellular_validation_of_the_interaction_between_TET2_and_Vpr_p_/34047911 CC BY 4.0