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<p>Structural and functional characterization of Vpr C-terminal region.</p>
Dianhong Wang (25157696)
Weijia Ding (554415)
Jingwei Xu (337549)
Ruofan Li (1568194)
Tamino Cairoli (25157699)
Qi Liu (33068)
Yiying Zhu (8675928)
Ye Xiang (522776)
Biophysics
Biochemistry
Cell Biology
Evolutionary Biology
Infectious Diseases
Plant Biology
Virology
various restriction factors
ring e3 ligase
larger tet2 fragment
findings could facilitate
diverse substrate recruitment
vprbp could enable
vprbp adopt either
xlink "> hiv
two &# 8220
recruits diverse substrates
bound host factors
like domains adopt
diverse vpr
host cul4
&# 8220
vprbp mediates
vprbp dimerization
receptor vprbp
&# 8221
structures highlight
structural basis
results pinpoint
molecular architecture
may assist
lish domain
key protein
guided development
divergent stoichiometries
directly interact
different vpr
conformational dynamics
accommodating vpr
1 vpr
1 hijacks
<p>(<b>A</b>) Density corresponding to Vpr C-terminus. A difference omit map shows the additional density near helix α2 of Vpr, which is almost parallel to helix α2 and has an average distance of 10 Å to the central axis of helix α2. (<b>B</b>) Functional validation of the Vpr C-terminal mutant. Western blot analysis showing that the Vpr C-terminal R88A mutant maintains its capacity to degrade UNG2. Western blots were performed in three independent biological replicates (<i>n</i> = 3) with one representative blot shown. Hsp90 was used as an internal loading control. Vpr mutant Q65R, which is deficient in VprBP binding, was used as a negative control. (<b>C)</b> Statistical quantification of biological replicates. Densitometric quantification derived from the three independent experimental repeats. Data are presented as mean. Error bars represent the standard deviation (SD). <i>n.s</i>., not statistically significant (<i>P</i> > 0.05); ***, <i>P</i> < 0.001.</p> <p>(TIF)</p>
2026-10-01T17:28:02Z
Image
Figure
10.1371/journal.ppat.1014610.s014
https://figshare.com/articles/figure/_p_Structural_and_functional_characterization_of_Vpr_C-terminal_region_p_/34047896
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