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oai:figshare.com:article/34047644 2026-10-01T17:26:16Z category_1 category_4 category_8 category_13 category_14 category_873 category_734 category_61 category_64 category_134 portal_5 item_type_1 month_year_10_2026

<p>Splicing inhibition did not preclude cotranscriptional recruitment of U1 snRNP and BBP to the <i>RPL22B</i> locus.</p> Kateřina Abrhámová (4739808) Alexandra Gredová (25157630) Karolína Navrátilová (25157633) Mohamed Boumaiza (5636147) Petr Folk (209900) Biophysics Biochemistry Microbiology Genetics Molecular Biology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Cancer Virology intergenic regulation mediated first catalytic step 5 ’ ss div >< p additional stem loops tested splicing efficiency saccharomyces cerevisiae </ rather stalled splicing enhanced splicing inhibition intron binding using predicted stem alternate stem p structure permitted splicing b </ structural ensemble rpl22 </ ribosomal roles ribosomal proteins regulatory mechanism prevent recognition predicted features particular type others described mutagenesis identified mrna behaves later stage hybrid system fulfill non containing reporter branch point binding capacity allosteric switch <p>The recruitment of spliceosomal components to the <i>RPL22B</i> locus was analyzed by ChIP-qPCR analysis. We used cells expressing HA-tagged Prp42, Msl5, and Mud2 to monitor the cotranscriptionality of the formation of commitment complex 1. The signals were normalized to input values and expressed as the fold enrichment relative to the signal obtained from the telomeric region (TEL VI R). A representative graph of three independent experiments is shown; error bars represent the S.D. of three technical replicates. To compare the effect of low and high concentrations of Rpl22, we used <i>rpl22a</i>Δ and <i>rpl22a</i>Δi strains, respectively. Positions of the PCR amplicons P to E are indicated.</p> 2026-10-01T17:25:45Z Image Figure 10.1371/journal.pone.0359197.g006 https://figshare.com/articles/figure/_p_Splicing_inhibition_did_not_preclude_cotranscriptional_recruitment_of_U1_snRNP_and_BBP_to_the_i_RPL22B_i_locus_p_/34047644 CC BY 4.0