2026-10-09T20:35:29Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34047635
2026-10-01T17:26:12Z
category_1
category_4
category_8
category_13
category_14
category_873
category_734
category_61
category_64
category_134
portal_5
item_type_1
month_year_10_2026
<p>Splicing-permissive mutations did not block <i>RPL22B</i>i splicing <i>in vivo</i> irrespective of the presence of the Rpl22 protein.</p>
Kateřina Abrhámová (4739808)
Alexandra Gredová (25157630)
Karolína Navrátilová (25157633)
Mohamed Boumaiza (5636147)
Petr Folk (209900)
Biophysics
Biochemistry
Microbiology
Genetics
Molecular Biology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Virology
intergenic regulation mediated
first catalytic step
5 ’ ss
div >< p
additional stem loops
tested splicing efficiency
saccharomyces cerevisiae </
rather stalled splicing
enhanced splicing inhibition
intron binding using
predicted stem
alternate stem
p structure
permitted splicing
b </
structural ensemble
rpl22 </
ribosomal roles
ribosomal proteins
regulatory mechanism
prevent recognition
predicted features
particular type
others described
mutagenesis identified
mrna behaves
later stage
hybrid system
fulfill non
containing reporter
branch point
binding capacity
allosteric switch
<p>The experiment was done and the figure was formatted as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0359197#pone.0359197.g002" target="_blank">Fig 2</a>. (A) Mutants disrupting the inhibitory conformation of <i>RPL22B</i>i were splicing permissive. See the legend of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0359197#pone.0359197.g002" target="_blank">Fig 2A</a>. (B) Summary of the reporter experiments in (A), Rpl22 binding data from the yeast three-hybrid assay, and types of stem loop arrangements predicted by RNAshapes. See the legend of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0359197#pone.0359197.g002" target="_blank">Fig 2B</a>.</p>
2026-10-01T17:25:45Z
Image
Figure
10.1371/journal.pone.0359197.g003
https://figshare.com/articles/figure/_p_Splicing-permissive_mutations_did_not_block_i_RPL22B_i_i_splicing_i_in_vivo_i_irrespective_of_the_presence_of_the_Rpl22_protein_p_/34047635
CC BY 4.0