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<p>Antigenicity of bat sarbecoviruses using monoclonal antibodies isolated from SARS-CoV-2 breakthrough infections.</p> Nazia Thakur (9904162) Joseph Newman (5638340) Dongchun Ni (15188356) Jeffrey Seow (9415776) Abigail L. Hay (25157576) Yeonjae Lee (12256119) Ayush Upadhyay (25157573) Babatunde E. Ekundayo (25157578) John A. Hammond (7431785) Thomas P. Peacock (6536120) Kelvin Lau (1847764) Katie J. Doores (346210) Dalan Bailey (193037) Microbiology Cell Biology Evolutionary Biology Environmental Sciences not elsewhere classified Ecology Immunology Biological Sciences not elsewhere classified Cancer Infectious Diseases Virology multiple bat coronaviruses g ., rc g ., civet clade 1 viruses restricted ace2 usage mammalian ace2 proteins g ., rhgb07 using monoclonal antibodies clade 1 sarbecoviruses div >< p determined host range converting enzyme 2 2 convalescent individuals ace2 ), via using binding assays antigenicity using sera related bat sarbecoviruses using sarbecoviruses host range clade 3 pangolin ), neutralising antibodies entry assays binding domain sarbecoviruses interact sarbecoviruses exhibited rodent ace2 like rhgb07 generalist ace2 related antigenicity zoonotic potential vitally important still evidence specialist </ shifting pattern robust evidence research indicates racoon dog novel structure largely maintained including human immunodominant target human primate generalist phenotypes generalist </ especially omicron entry phenotypes diverse panel continued evolution breakthrough infections <p><b>(A)</b> Dissociation constant (K_D <b>(M)</b>, 2−4 replicates <u>+</u> sd) of mAb binding kinetics calculated by SPR (bars) and ELISA EC₅₀ (µg/ml, 2 replicates <u>+</u> sd) values (broken lines with filled symbols) for a subset of mAbs and sarbecovirus RBDs. For K_D, no bar indicates no binding by SPR. For EC₅₀, lower limit of detection: <0.08 µg/ml, upper limit of detection: >5 µg/ml. <b>(B)</b> Neutralisation (IC₅₀) titres shown for each mAb against bat sarbecovirus pseudotyped viruses (µg/ml), with limits of detection indicated (lower limit: <0.08 µg/ml; upper limit: >50 µg/ml for IC₅₀). HEK293T cells stably expressing human ACE2 were used for all neutralisation assays. apart from RhGB07 and Rc-o319, where HEK293T cells overexpressing <i>Rh. alcyone</i> or <i>Rh. cornutus</i> were used, respectively. Scale bars (right) represent direction of increased binding or neutralisation. <b>(C)</b> RhGB07 trimer and RBD, with antibody competition groups highlighted, and are coloured with the same colouring as the SARS-CoV-2 trimer in (<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003944#pbio.3003944.g006" target="_blank">Fig 6B</a>). Regions of divergence in binding epitopes in RhGB07 compared to SARS-CoV-2 are depicted, with amino acid residues denotes. Colours in all graphs/structures indicate mAb binding competition groups (Group 1 = purple, Group 2 = orange, Group 3 = red, Group 3.5 = blue, Group 4 = green). EC₅₀, K_D and IC₅₀ values be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003944#pbio.3003944.s016" target="_blank">S4 Table</a>. The data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003944#pbio.3003944.s017" target="_blank">S1 Data</a>.</p> 2026-10-01T17:40:07Z Image Figure 10.1371/journal.pbio.3003944.g007 https://figshare.com/articles/figure/_p_Antigenicity_of_bat_sarbecoviruses_using_monoclonal_antibodies_isolated_from_SARS-CoV-2_breakthrough_infections_p_/34047385 CC BY 4.0