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<p>Bat sarbecovirus usage of mammalian ACE2.</p>
Nazia Thakur (9904162)
Joseph Newman (5638340)
Dongchun Ni (15188356)
Jeffrey Seow (9415776)
Abigail L. Hay (25157576)
Yeonjae Lee (12256119)
Ayush Upadhyay (25157573)
Babatunde E. Ekundayo (25157578)
John A. Hammond (7431785)
Thomas P. Peacock (6536120)
Kelvin Lau (1847764)
Katie J. Doores (346210)
Dalan Bailey (193037)
Microbiology
Cell Biology
Evolutionary Biology
Environmental Sciences not elsewhere classified
Ecology
Immunology
Biological Sciences not elsewhere classified
Cancer
Infectious Diseases
Virology
multiple bat coronaviruses
g ., rc
g ., civet
clade 1 viruses
restricted ace2 usage
mammalian ace2 proteins
g ., rhgb07
using monoclonal antibodies
clade 1 sarbecoviruses
div >< p
determined host range
converting enzyme 2
2 convalescent individuals
ace2 ), via
using binding assays
antigenicity using sera
related bat sarbecoviruses
using sarbecoviruses
host range
clade 3
pangolin ),
neutralising antibodies
entry assays
binding domain
sarbecoviruses interact
sarbecoviruses exhibited
rodent ace2
like rhgb07
generalist ace2
related antigenicity
zoonotic potential
vitally important
still evidence
specialist </
shifting pattern
robust evidence
research indicates
racoon dog
novel structure
largely maintained
including human
immunodominant target
human primate
generalist phenotypes
generalist </
especially omicron
entry phenotypes
diverse panel
continued evolution
breakthrough infections
<p><b>(A)</b> Heatmap showing the receptor usage of bat sarbecovirus-pseudotyped viruses with mammalian ACE2s, including human, livestock/pets, rodents, potential intermediate hosts and monkeys. The mean log RLU from 3 separate experiments is shown and a pDISPLAY control to illustrate the background levels of entry. <b>(B)</b> Surface representation of human ACE2 coloured with mammalian ACE2 conservation (human ACE2: PDB 6M0J), with the outlined region denoting the RBD-interacting sites, with individual amino acids labelled. <b>(C)</b> WebLogo (University of California, Berkley, USA) plots summarising the amino acid divergence within the mammalian ACE2 sequences used in this screen. The single-letter amino acid code is used with the vertical height presenting the relative frequency of that amino acid at each position. The positions at which ACE2 interacts with SARS-CoV-2 RBD are shown, N-terminal to C-terminal, with different colours representing amino acid conservation between the mammalian ACE2s examined in this study. The data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003944#pbio.3003944.s017" target="_blank">S1 Data</a>.</p>
2026-10-01T17:40:07Z
Image
Figure
10.1371/journal.pbio.3003944.g003
https://figshare.com/articles/figure/_p_Bat_sarbecovirus_usage_of_mammalian_ACE2_p_/34047373
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