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<p>GFP+ syncytia formation of bat sarbecovirus entry by cell–cell fusion.</p>
Nazia Thakur (9904162)
Joseph Newman (5638340)
Dongchun Ni (15188356)
Jeffrey Seow (9415776)
Abigail L. Hay (25157576)
Yeonjae Lee (12256119)
Ayush Upadhyay (25157573)
Babatunde E. Ekundayo (25157578)
John A. Hammond (7431785)
Thomas P. Peacock (6536120)
Kelvin Lau (1847764)
Katie J. Doores (346210)
Dalan Bailey (193037)
Microbiology
Cell Biology
Evolutionary Biology
Environmental Sciences not elsewhere classified
Ecology
Immunology
Biological Sciences not elsewhere classified
Cancer
Infectious Diseases
Virology
multiple bat coronaviruses
g ., rc
g ., civet
clade 1 viruses
restricted ace2 usage
mammalian ace2 proteins
g ., rhgb07
using monoclonal antibodies
clade 1 sarbecoviruses
div >< p
determined host range
converting enzyme 2
2 convalescent individuals
ace2 ), via
using binding assays
antigenicity using sera
related bat sarbecoviruses
using sarbecoviruses
host range
clade 3
pangolin ),
neutralising antibodies
entry assays
binding domain
sarbecoviruses interact
sarbecoviruses exhibited
rodent ace2
like rhgb07
generalist ace2
related antigenicity
zoonotic potential
vitally important
still evidence
specialist </
shifting pattern
robust evidence
research indicates
racoon dog
novel structure
largely maintained
including human
immunodominant target
human primate
generalist phenotypes
generalist </
especially omicron
entry phenotypes
diverse panel
continued evolution
breakthrough infections
<p><b>(A)</b> GFP and brightfield images of cell-cell fusion of representative bat sarbecovirus Spikes (transfected into HEK293T effector cells stably expressing rLuc-GFP 1–7) with bat ACE2s (transfected into BHK-21 cells expressing rLuc-GFP 8–11) 24 h after co-culture. Four fields of view were taken per well at 10x magnification. Scale bar is shown, 400 µm. <b>(B)</b> Analysis of images generated was performed using the IncuCyte S2 software, calculating the total sum of object with fluorescence above the background threshold, measured as total integrated intensity, and expressed as green count units (GCU) µm<sup>2</sup> and shown in a heatmap. The data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003944#pbio.3003944.s017" target="_blank">S1 Data</a>.</p> <p>(TIF)</p>
2026-10-01T17:40:07Z
Image
Figure
10.1371/journal.pbio.3003944.s004
https://figshare.com/articles/figure/_p_GFP_syncytia_formation_of_bat_sarbecovirus_entry_by_cell_cell_fusion_p_/34047322
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