2026-10-14T13:57:31Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34046898 2026-10-01T16:07:57Z category_1 category_4 category_146 category_915 category_7 category_14 category_21 category_873 category_734 category_64 category_135 portal_63 item_type_6 month_year_10_2026

Resolving DNA Conformations and Dynamics at the Graphene Interface by Graphene Energy Transfer Chenyuan Yan (15333515) Jakob Hartmann (511537) Lars Richter (401625) Giovanni Ferrari (4493617) Johanna Eisenbeil (25157400) Tim Schröder (1640023) Deep Sekhar Biswas (7894151) Izabela Kamińska (8562477) Alan M. Szalai (10183409) Philip Tinnefeld (1418620) Biophysics Biochemistry Space Science Physical Sciences not elsewhere classified Medicine Molecular Biology Biotechnology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Cancer Computational Biology passive quenching surface resolving dna conformations probing molecular interfaces molecule dna nanoelevator vertical nucleic acids results establish getvna graphene energy transfer graphene binding sites engineering dna molecule readout molecular materials molecular level unzipping events recent discovery parallel orientations pair zipping opening opportunities nanoscale devices nanometer precision exposed nucleotides dsdna adopts displacement reactions based nanodevices The recent discovery that double-stranded DNA stands vertically on graphene has transformed graphene from a passive quenching surface into an active platform for axial single-molecule readout, opening opportunities in biosensing, molecular materials, and nanoscale devices. Here, we use graphene energy transfer with vertical nucleic acids (GETvNA) to resolve how partially single- and double-stranded DNA constructs interact with graphene at the molecular level. Single-molecule fluorescence lifetime measurements show that dsDNA adopts both vertical and parallel orientations on graphene and reveal rare 1–3 base-pair zipping and unzipping events. We further show that the ssDNA anchor length regulates dsDNA conformation by controlling the availability of graphene binding sites for exposed nucleotides, thereby modulating partial unzipping. Finally, we demonstrate programmable axial motion using DNA hybridization and strand-displacement reactions, realizing a single-molecule DNA nanoelevator with nanometer precision. These results establish GETvNA as a platform for probing molecular interfaces and engineering DNA-based nanodevices on graphene. 2026-10-01T00:00:00Z Text Journal contribution 10.1021/acs.nanolett.6c03383.s001 https://figshare.com/articles/journal_contribution/Resolving_DNA_Conformations_and_Dynamics_at_the_Graphene_Interface_by_Graphene_Energy_Transfer/34046898 CC BY-NC 4.0