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oai:figshare.com:article/34046784 2026-10-01T15:56:14Z category_8 portal_316 item_type_1 month_year_10_2026

Image 2_Etiology-associated differences in the gut microbiome and metabolome in hepatocellular carcinoma: an exploratory study of alcohol-related and HBV-related cases.tif Yongguang Wei (17145991) Kejian Yang (7027397) Qinchen Lu (11064702) Xin Zhou (54275) Huasheng Huang (3425024) Meiliang Liu (8902670) Lijun Wang (176511) Liming Shang (25141257) Tao Peng (81319) Xiaoyun Zeng (698516) Microbiology alcohol-related liver disease gut microbiome hepatitis B virus hepatocellular carcinoma metabolomics <p>Hepatocellular carcinoma (HCC) arises in heterogeneous etiological contexts, but direct cross-omics comparisons between HCC subgroups defined by different etiologies remain limited. In this exploratory study, we combined fecal 16S rRNA gene sequencing and untargeted serum metabolomics with exploratory cross-omics analyses to compare gut microbial composition, serum metabolic profiles, and microbiome–metabolite association patterns between hepatitis B virus-related HCC (HBV-HCC) and alcohol-related HCC (ALD-HCC). Forty patients who underwent primary tumor resection were included, comprising 26 with HBV-HCC and 14 with ALD-HCC. Alpha diversity indices did not differ significantly between groups, whereas within-group Bray–Curtis dissimilarity was greater in ALD-HCC and genus-level beta-diversity ordination showed only modest separation between the two groups. LEfSe-based screening suggested the enrichment of candidate taxa, including Faecalibacterium, Roseburia, Lachnospira, Turicibacter, and Butyribacter in HBV-HCC, whereas Allisonella and Coprobacillus were enriched in ALD-HCC. Untargeted metabolomics showed group separation in supervised models and identified exploratory candidate metabolic features mainly involving glycerophospholipid metabolism, primary bile acid biosynthesis, aminoacyl-tRNA biosynthesis, and central carbon metabolism in cancer; however, these metabolite-level findings did not remain significant after multiple-testing correction. Procrustes and O2PLS analyses indicated limited global concordance between the microbiome and metabolome, although exploratory associations were observed between several phosphatidylcholine-related features and microbial taxa. Overall, HBV-HCC and ALD-HCC may be associated with different gut microbial and serum metabolic patterns, and these findings should be considered exploratory and hypothesis-generating.</p> 2026-10-01T15:56:14Z Image Figure 10.3389/fmicb.2026.1927595.s003 https://figshare.com/articles/figure/Image_2_Etiology-associated_differences_in_the_gut_microbiome_and_metabolome_in_hepatocellular_carcinoma_an_exploratory_study_of_alcohol-related_and_HBV-related_cases_tif/34046784 CC BY 4.0