2026-10-10T19:36:31Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34046550 2026-10-01T15:15:32Z category_8 portal_316 item_type_3 month_year_10_2026

Data Sheet 2_Effect of environmental conditions on two vibriophages and their host.pdf Marcela León (6860729) Neri Vargas (24408927) Kívely Lozano (25157124) Santiago Gacitúa (25157127) Carlos Omar Lomelí-Ortega (25157130) Irais Ramírez-Sánchez (14561810) Eduardo Quiroz-Guzmán (25157133) Roberto Bastías (3181728) Microbiology bacteriophage environmental performance Vibrio Vibrio anguillarum Vibrio parahaemolyticus vibriophages <p>Bacteriophages play a key role in regulating Vibrio populations in marine environments, and environmental conditions may influence their replication and interactions with bacterial hosts. This study aimed to isolate and characterize the bacteriophage PvVp2, which infects the pandemic Vibrio parahaemolyticus strain KX, and to compare its environmental performance with that of phage CHOED, a lytic phage infecting Vibrio anguillarum. Phage PvVp2 possesses a 43,158 bp double-stranded DNA genome encoding a MazG-like pyrophosphatase, a putative auxiliary metabolic gene. Phylogenetic analysis clustered PvVp2 with vibriophages vB_Vc_SrVc2 and vB_Vc_SrVc9, sharing nearly 100% nucleotide identity. To evaluate the effect of environmental conditions on phage–host interactions, the replication of both phages was assessed under different temperatures, pH values and salinities. Performance curves showed that V. parahaemolyticus and PvVp2 were adapted to warmer conditions than V. anguillarum and CHOED, respectively. PvVp2 replicated efficiently between 22 °C and 40 °C, whereas CHOED replication was restricted to 14 °C–30 °C. Both phages replicated over narrower ranges of environmental conditions than their bacterial hosts, and deviations from optimal conditions increased latent periods and reduced burst sizes. Infection assays further showed that CHOED displayed greater lytic activity at 14 and 25 °C, whereas PvVp2 exhibited a lower but relatively stable lytic activity across the temperatures evaluated. These findings demonstrate that temperature, pH and salinity strongly influence phage replication and phage–host interactions, highlighting that the environmental range supporting phage replication is generally narrower than that supporting bacterial growth.</p> 2026-10-01T15:15:32Z Dataset Dataset 10.3389/fmicb.2026.1914226.s002 https://figshare.com/articles/dataset/Data_Sheet_2_Effect_of_environmental_conditions_on_two_vibriophages_and_their_host_pdf/34046550 CC BY 4.0