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oai:figshare.com:article/34046528 2026-10-01T15:11:53Z category_4 category_12 category_14 category_19 category_21 category_873 category_39 category_734 category_64 category_69 portal_63 item_type_6 month_year_10_2026

Pluronic F127 Micelles Co-encapsulating a Brachydin-Enriched Fraction and Curcumin: A Self-assembled Platform for Light-assisted Antileishmanial Activity Ingrid Maria Ribeiro de Lima (25157105) Aline Santana Figueredo (25157108) Lucilene Amorim Silva (9358637) Luciana Magalhães Rebelo Alencar (20424835) Cláudia Quintino da Rocha (25157111) Renato Sonchini Gonçalves (8720622) Biochemistry Cell Biology Molecular Biology Pharmacology Biotechnology Chemical Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Cancer Inorganic Chemistry purified individual brachydins polydisperse colloidal profile hydrophobic active materials high apparent active formal selectivity analysis dls analysis indicated dependent viability reductions 63 μg ml 33 μg ml assisted antileishmanial evaluation assisted antileishmanial activity intracellular amastigote assays associative pluronic f127 associated antileishmanial activity higher formulation concentrations 50 </ sub enriched fraction rather based formulation co antileishmanial assays interpretative f127 associated response enriched fraction fraction standardization without establishing vibrational environment therapeutic efficacy study developed still required related changes preliminary formulation material recovery level effects highest potency ftir data formulations containing dried samples data support dark conditions biological responses assembly model assembled platform approximately 406 approximately 12 5 nm 1 nm This study developed an associative Pluronic F127-based formulation co-loaded with a brachydin-enriched fraction and curcumin (FBC) for preliminary light-assisted antileishmanial evaluation. The formulation showed high apparent active-material recovery after centrifugation and filtration, as estimated by HPLC-PDA. DLS analysis indicated a polydisperse colloidal profile, with an apparent mean hydrodynamic diameter of approximately 406.5 nm and a number-weighted smaller population centered at approximately 12.1 nm. FTIR data were consistent with formulation-related changes in the vibrational environment of the hydrophobic active materials, while AFM and SEM provided complementary morphological information on deposited or dried samples. Together, these data support an interpretative F127-based self-assembly model, without establishing a fully uniform colloidal population or a definitively resolved internal micellar architecture. In vitro cytotoxicity assays using GM07492A human lung fibroblasts indicated low basal cytotoxicity under dark conditions, whereas light exposure produced concentration-dependent viability reductions at higher formulation concentrations. Antileishmanial assays against Leishmania amazonensis promastigotes revealed a marked light-associated response, mainly in formulations containing the brachydin-enriched fraction. FB showed the highest potency under light exposure, with a formulation-based IC₅₀ of 18.63 μg mL^–1, whereas FBC showed an IC₅₀ decrease from 72.74 to 34.33 μg mL^–1. Thus, although FBC was not more potent than FB, it combined light-associated antileishmanial activity, high apparent active-material recovery, and favorable macroscopic formulation behavior. Because FB and FBC contain a brachydin-enriched fraction rather than purified individual brachydins, the biological responses should be interpreted as preliminary formulation-level effects. Further studies involving direct reactive oxygen species measurements, intracellular amastigote assays, formal selectivity analysis, fraction standardization, and long-term colloidal stability evaluation are still required before therapeutic efficacy can be inferred. 2026-10-01T00:00:00Z Text Journal contribution 10.1021/acsomega.6c05278.s001 https://figshare.com/articles/journal_contribution/Pluronic_F127_Micelles_Co-encapsulating_a_Brachydin-Enriched_Fraction_and_Curcumin_A_Self-assembled_Platform_for_Light-assisted_Antileishmanial_Activity/34046528 CC BY-NC 4.0