2026-10-14T02:42:41Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34045992 2026-10-01T13:37:07Z category_23818 item_type_3 month_year_10_2026

Data for Artemisia Maryam Allahdou (25156050) Agricultural biotechnology diagnostics (incl. biosensors) artemisinin component <p dir="ltr">The genus Artemisia is of great medicinal importance. Tissue culture technologies and elicitors have been considered as solutions to increase secondary metabolite production. In this study, seeds and aerial parts of <i>Artemisia sieberi</i> were collected from the Sangan region of Khash County. Calluses were produced from leaf and stem explants in MS medium containing 0.5 mg/L BAP and 0.5 mg/L NAA, and then treated with L- ascorbic acid (0, 50, 100, and 200 mg/L). Cell suspension culture was treated with cellulase (0, 100, and 200 µg/L), and sampling was performed on days 1, 3, and 5. Artemisinin content were evaluated by HPLC, and essential oil compositions of aerial parts and mature fruit were evaluated by GC-MS. The results showed that the 100 mg/L, L-ascorbic acid treatment led to the greatest increase in artemisinin compared to the control. The use of cellulase also increased artemisinin in cell suspension cultures, but this increase was not comparable to that in plants grown in natural habitats. GC-MS analysis showed that monoterpenes were the dominant constituents of the essential oil in both organs, and Eucalyptol, Camphor, 3-thujanone, Artemisia alcohol, and Yomogi alcohol were among the most important compounds identified. Sesquiterpenes accounted for 1.818% and 4.66% of the total compounds identified in the essential oil of mature fruit and aerial organs, respectively. This study showed that L-ascorbic acid, can effectively increase artemisinin production under in vitro culture conditions. Additionally, the essential oil's richness in monoterpenes suggests potential biological relevance based on previously reported activities.</p> 2026-10-01T13:37:07Z Dataset Dataset 10.6084/m9.figshare.34045992.v1 https://figshare.com/articles/dataset/Data_for_Artemisia/34045992 CC BY 4.0