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oai:figshare.com:article/34045570 2026-10-01T12:05:51Z category_1 category_4 category_915 category_7 category_8 category_12 category_21 category_39 category_132 portal_63 item_type_6 month_year_10_2026

ε‑Poly‑l‑lysine-Functionalized 2D MoS₂ Nanosheets for Photothermal Inhibition of Cold-Adapted Shewanella putrefaciens Erwen Wu (25156438) Qingyi Wei (106811) Qihong Ouyang (25156441) Hongbin Pu (5844011) Lunjie Huang (4408393) Biophysics Biochemistry Physical Sciences not elsewhere classified Medicine Microbiology Cell Biology Biotechnology Ecology Infectious Diseases triggering protein leakage triggered mild hyperthermia synergistic antimicrobial partner shewanella putrefaciens </ robust biofilm formation pka ∼ 10 log cfu reduction induce deep penetration fluidized membranes rich density positive charges complete biofilm removal cationic surface modifier accelerating membrane damage 40 ° c resistant psychrotrophic pathogens listed food preservative adapted membrane dynamics functionalized 2d mos cold chain preservation 2 </ sub unique vulnerability strike mechanism room temperature physicochemical dual photothermal inhibition lose potency label solution green exfoliant engineering approach duty agent combat antibiotic adapted bacteria >, notorious Psychrotrophic <i>Shewanella putrefaciens</i>, notorious for dominating cold chain spoilage through cold-adapted membrane dynamics and robust biofilm formation, challenges conventional antimicrobial strategies that lose potency under refrigeration and raise food safety concerns. We report an engineering approach that transforms ε-poly-l-lysine (ε-PL), a GRAS-listed food preservative, into a triple-duty agent: a green exfoliant for MoS₂ nanosheets, a cationic surface modifier, and a synergistic antimicrobial partner. The engineered ε-PL@MoS₂ nanosheets exploit a unique vulnerability of cold-adapted bacteria, where their fluidized membranes rich in unsaturated phospholipids become prime targets for ε-PL’s high-density positive charges (pKa∼10.5), which induce deep penetration and disruption. NIR-triggered mild hyperthermia (40 °C) amplifies this effect by destabilizing lipid phase transitions, accelerating membrane damage, and triggering protein leakage, creating a physicochemical dual-strike mechanism that achieves exceptional bactericidal performance (5.2-log CFU reduction) and near-complete biofilm removal (99.7%) within 10 min at room temperature. This intelligent, food-safe nanoplatform bridges nanoscience and cold chain preservation, offering a clean-label solution to combat antibiotic-resistant psychrotrophic pathogens. 2026-10-01T00:00:00Z Text Journal contribution 10.1021/acs.inorgchem.6c03028.s001 https://figshare.com/articles/journal_contribution/_Poly_l_lysine-Functionalized_2D_MoS_sub_2_sub_Nanosheets_for_Photothermal_Inhibition_of_Cold-Adapted_Shewanella_putrefaciens/34045570 CC BY-NC 4.0