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oai:figshare.com:article/34044498 2026-10-01T11:18:36Z category_1 category_4 category_7 category_12 category_19 category_21 category_873 category_61 category_64 portal_63 item_type_6 month_year_10_2026

Acoustochromatography Based on Low-Cost Polymer Columns Enables Rapid Extracellular Vesicle Proteome Analysis from 1 μL of Blood Plasma Michael S. Gerlt (20873362) Melinda Rezeli (647893) Thomas Laurell (72950) Biophysics Biochemistry Medicine Cell Biology Pharmacology Biotechnology Chemical Sciences not elsewhere classified Developmental Biology Cancer absolute residual concentration ev isolation methods enables ev enrichment early disease detection based acoustochromatography device acoustochromatography based standard methods acoustofluidic enrichment volume samples values reported poorly compatible plasma background novel ultrasound neat plasma hold promise current gold conventional approaches clinical workflows associated proteins 100 ng 1 μl Extracellular vesicles (EVs) hold promise as biomarkers for early disease detection, yet their nanoscale size and the protein-rich plasma background make enrichment essential. Conventional approaches such as ultracentrifugation and size-exclusion chromatography require ≥100 μL of input, hours of processing, and are poorly compatible with clinical workflows. We present a low-cost polymer-based acoustochromatography device that enables EV enrichment from 1 μL of blood plasma within 2–10 minsignificantly faster than current gold-standard methods. A novel ultrasound-driven release mode increased recovery significantly compared to the previously reported acoustofluidic chromatography method. Comprehensive proteomic analysis confirmed highly pure EV fractions after acoustofluidic enrichment. Apolipoproteins were depleted to below 100 ng/mL, corresponding to >99.6% depletion as compared to neat plasma. This absolute residual concentration is lower than values reported for other EV isolation methods and enabled the detection of an additional 689 mostly EV-associated proteins above the plasma background. This low-cost, scalable platform unlocks proteomic profiling of ultralow-volume samples, paving the way for rapid and clinically compatible EV biomarker diagnostics. 2026-10-01T00:00:00Z Text Journal contribution 10.1021/acs.analchem.6c03940.s001 https://figshare.com/articles/journal_contribution/Acoustochromatography_Based_on_Low-Cost_Polymer_Columns_Enables_Rapid_Extracellular_Vesicle_Proteome_Analysis_from_1_L_of_Blood_Plasma/34044498 CC BY-NC 4.0