2026-10-14T14:17:28Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34041876 2026-10-01T09:38:24Z category_21 portal_316 item_type_3 month_year_10_2026

Supplementary file 1_Proof-of-concept validation and characterisation of a DLP 3D bioprinted vascularised colorectal cancer model.docx Atanas Valev (25155741) Yordan Sbirkov (10303190) David Liebl (634602) Eva Ďurinová (23694166) Daniela Popelková (25155744) Aleš Benda (2522944) Michael Ausserlechner (20346453) Murad Redzheb (25155747) Victoria Sarafian (10303205) Biotechnology channels colorectal cancer DLP bioprinting GelMA HCT-116 HUVEC vascularisation <p>Tumor vascularisation is essential in promoting cancer growth and metastasis and represents a key target for efficient drug delivery. Nevertheless, in vitro experimental models are limited to monolayer (co)cultures of endothelial and malignant cells, scratch and transwell migration assays where the vascularisation process is largely neglected. Direct contacts between different cell types and extracellular matrix (ECM) components, and processes like cell migration and invasion and ECM remodeling are also difficult to reproduce using conventional culturing methods. This study aims to address some of these limitations by developing digital light processing (DLP) 3D bioprinted model of colorectal cancer based on methacrylated gelatin (GelMA) and containing endothelialised channels. The materials and approaches proposed here were additionally validated with 3D biofabricated in-house made designs based on the (bio)casting of GelMA and collagen hydrogels, and with 3D printed transwell assays. We found that our DLP bioprinting and 3D biofabrication protocols support the growth of HCT-116 colorectal cancer cells (embedded in the gel) and the survival of HUVEC endothelial cells (seeded to the channels), that cells adhere well to the collagen and GelMA matrices, and that the cells can remodel the hydrogel as revealed by scanning electron microscopy (SEM). Importantly, we also found that HUVECs migrate through these substrates in both the channel and transwell constructs. Altogether, to our knowledge, this pilot study presents the first DLP bioprinted model of CRC using GelMA, and the only DLP bioprinted colorectal cancer model seeded with HUVECs suitable for in vitro study of tumor vascularisation and drug delivery.</p> 2026-10-01T09:38:24Z Dataset Dataset 10.3389/fbioe.2026.1944341.s001 https://figshare.com/articles/dataset/Supplementary_file_1_Proof-of-concept_validation_and_characterisation_of_a_DLP_3D_bioprinted_vascularised_colorectal_cancer_model_docx/34041876 CC BY 4.0