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Chlorantraniliprole
Suppresses the Humoral Immunity
in Spodoptera frugiperda Larvae by
Downregulating Peptidoglycan Recognition Protein Gene SfPGRP-S5
Qingyan Liu (12512598)
Wenqi Xie (5903936)
Jiaxin Liu (833821)
Shuhua Liu (1598038)
Xiaoyue Deng (15254045)
Qing Li (84975)
Yanli Yue (12706836)
Huabao Chen (691884)
Qunfang Yang (5931560)
Chunxian Jiang (5931557)
Biochemistry
Microbiology
Genetics
Molecular Biology
Environmental Sciences not elsewhere classified
Ecology
Immunology
Biological Sciences not elsewhere classified
Developmental Biology
Infectious Diseases
reduced bacterial susceptibility
provides new insights
peptidoglycan recognition proteins
downregulated antimicrobial peptide
differential immune regulation
modulating humoral responses
functional studies revealed
spodoptera frugiperda larvae
s5 activated amp
s5 </ italic
frugiperda larvae
s5 </
previous studies
findings revealed
humoral immunity
drosophila </
study reveals
significantly affected
mediating chlorantraniliprole
key mediators
induced suppression
chlorantraniliprole suppresses
bioassay indicated
Previous studies have demonstrated that chlorantraniliprole
enhances
cellular immunity in Spodoptera frugiperda larvae, but its effects on humoral immunity remain unclear. Thus,
we investigated the role of this insecticide in modulating humoral
responses, focusing on peptidoglycan recognition proteins (PGRPs)
as key mediators. PGRPs are important pattern recognition receptors
that activate insect innate immunity against microbes. Our findings
revealed that chlorantraniliprole inhibited humoral immunity in larvae,
with the expression of S<i>fPGRP-S5</i> being significantly
affected. Functional studies revealed that <i>SfPGRP-S5</i> knockdown under chlorantraniliprole exposure decreased larval survival
following bacterial infection and downregulated antimicrobial peptide
(AMP) genes, while recombinant SfPGRP-S5 activated AMP and prophenoloxidase
(PPO) expression. Additionally, the transgenic <i>Drosophila</i> bioassay indicated that <i>SfPGRP-S5</i> overexpression
enhanced chlorantraniliprole tolerance and reduced bacterial susceptibility.
In summary, <i>SfPGRP-S5</i> plays a critical role in mediating
chlorantraniliprole-induced suppression of humoral immunity in S. frugiperda larvae. This study reveals the differential
immune regulation by insecticides and provides new insights for understanding
their sublethal effects.
2026-10-01T00:00:00Z
Text
Journal contribution
10.1021/acs.jafc.6c08585.s001
https://figshare.com/articles/journal_contribution/Chlorantraniliprole_Suppresses_the_Humoral_Immunity_in_Spodoptera_frugiperda_Larvae_by_Downregulating_Peptidoglycan_Recognition_Protein_Gene_SfPGRP-S5/34041674
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