2026-10-11T22:37:29Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34041471
2026-10-01T08:11:42Z
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Chemical and Proteomic
Characterization of Bioactive
Protein-Enriched Fraction from Schizophyllum commune with Anticancer Activity in HeLa Cells
Saranya Sedtananun (25155333)
Lueacha Tabtimmai (12554632)
Chanapat Butsukon (25155336)
Chanikan Sonklin (22981231)
Kiattawee Choowongkomon (122141)
Biophysics
Biochemistry
Physical Sciences not elsewhere classified
Molecular Biology
Physiology
Pharmacology
Chemical Sciences not elsewhere classified
Developmental Biology
Cancer
Hematology
treated hela cells
split gill mushroom
showing minimal effects
physicochemical profiling demonstrated
normal hacat cells
lc – ms
isoelectric points ranging
findings provide insight
exchange chromatography coupled
edible fungus recognized
diverse biological processes
colony formation assays
guided fractionation studies
>- derived protein
enriched fraction f2
50 </ sub
heterogeneous protein composition
enriched fraction
proteomic composition
guided approach
commune </
enriched fractions
using ion
schizophyllum commune
results support
proteomic characterization
potential applications
fruiting bodies
fluorescence staining
flow cytometry
cytotoxic activity
commonly known
biotechnology industries
bioactive proteins
bioactive compounds
anticancer activity
37 μg
Schizophyllum commune,
commonly
known as the split gill mushroom, is an edible fungus recognized as
a source of bioactive compounds with potential applications in the
food and biotechnology industries. In this study, protein-enriched
fractions were isolated from the fruiting bodies of <i>S. commune</i> using ion-exchange chromatography coupled with a bioassay-guided
approach. Cytotoxic activity was evaluated in HeLa cells using MTT
and colony formation assays. Among the fractions, the protein-enriched
fraction F2 exhibited antiproliferative activity with an IC<sub>50</sub> value of 0.37 μg/mL and significantly inhibited colony formation,
while showing minimal effects on normal HaCaT cells. Flow cytometry
and fluorescence staining further supported the involvement of apoptosis
in F2-treated HeLa cells. Chemical and proteomic characterization
of the protein-enriched fraction F2 by SDS-PAGE and LC–MS/MS
identified 64 protein groups. Gene ontology analysis revealed proteins
involved in diverse biological processes, while physicochemical profiling
demonstrated a heterogeneous protein composition with isoelectric
points ranging from 4 to 7. These findings provide insight into the
chemical and proteomic composition of protein-enriched fraction F2.
The results support further investigation of <i>S. commune</i>-derived protein-enriched fractions as a source of bioactive proteins,
particularly in bioactivity-guided fractionation studies.
2026-10-01T00:00:00Z
Dataset
Dataset
10.1021/acsomega.6c03766.s001
https://figshare.com/articles/dataset/Chemical_and_Proteomic_Characterization_of_Bioactive_Protein-Enriched_Fraction_from_Schizophyllum_commune_with_Anticancer_Activity_in_HeLa_Cells/34041471
CC BY-NC 4.0