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oai:figshare.com:article/34041296 2026-10-01T07:25:01Z category_37339 category_37354 category_37366 portal_1012 item_type_6 month_year_10_2026

Supplementary Figure 2 from The H63D Polymorphism in the <i>HFE</i> Gene Promotes Aggressive Disease Phenotypes in Pancreatic Ductal Adenocarcinoma Federica Rubbino (25155083) Luana Greco (25155086) Alessandro Scagliotti (25155089) Alessio Di Cristofaro (25155092) Valentina de Murtas (25155095) Gabriele De Simone (25155098) Stefania Forciniti (25155101) Fabio Grizzi (15125329) Paolo Bianchi (25155104) Gianluca Basso (15137545) Luca Lambroia (25155107) Paolo Kunderfranco (15137566) Paola Cappello (15089919) Francesco Novelli (25155110) Claudio Doglioni (25155113) Piergiuseppe Colombo (25155116) Massimiliano Mazzone (15136493) Massimo Falconi (25155119) Alessandro Zerbi (24694352) Alberto Malesci (25155122) Luigi Laghi (25155125) Cancer Molecular and Cellular Biology Clinical Research and Trials Cell Cycle Control of cell cycle progression Gastrointestinal Cancers Pancreatic cancer Precision Medicine <p>(A) Representative immunohistochemical staining for Ki67 performed on FFPE human PDAC specimens from wild-type/non-carrier patients (WT, <i>n</i> = 57) and H63D carriers (<i>n</i> = 25) and (B) quantification of Ki67-positive area. (C) Representative Western blot analysis of HFE protein expression in FFPE PDAC tissues from WT patients and H63D carriers (including both heterozygous and homozygous cases). (D) Densitometric quantification of HFE band intensity. Values are presented as median ±SD. (E) Distribution of loss of heterozygosity (LOH) fractions at the <i>HFE</i> locus (chromosome 6) across PDAC samples. LOH was calculated using germline-informative heterozygous sites and matched tumor sequencing data, integrating genotype calls and allele-specific read counts. The distribution is strongly skewed toward low values, with most samples showing minimal or no allelic imbalance. Mean (dashed red line) and median (dotted green line) LOH fractions are indicated. These data indicate that LOH at the <i>HFE</i> locus is infrequent and not recurrent in PDAC.</p> 2026-10-01T00:00:00Z Text Journal contribution 10.1158/1078-0432.34041296 https://figshare.com/articles/journal_contribution/Supplementary_Figure_2_from_The_H63D_Polymorphism_in_the_i_HFE_i_Gene_Promotes_Aggressive_Disease_Phenotypes_in_Pancreatic_Ductal_Adenocarcinoma/34041296 CC BY